Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines
Clinacanthus nutans Lindau leaves (CN) have been used in traditional medicine but the therapeutic potential has not been explored for cancer prevention and treatment. Current study aimed to evaluate the antioxidant and antiproliferative effects of CN, extracted in chloroform, methanol, and water, on...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29551/1/Clinacanthus%20nutans%20Extracts%20Are%20Antioxidant%20with.pdf |
| _version_ | 1796971562123919360 |
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| author | Yong, Yoke Keong Tan, Jun Jie Teh, Soek Sin Mah, Siau Hui Ee, Gwendoline Cheng Lian Chiong, Hoe Siong Ahmad, Zuraini |
| author_facet | Yong, Yoke Keong Tan, Jun Jie Teh, Soek Sin Mah, Siau Hui Ee, Gwendoline Cheng Lian Chiong, Hoe Siong Ahmad, Zuraini |
| author_sort | Yong, Yoke Keong |
| collection | UPM |
| description | Clinacanthus nutans Lindau leaves (CN) have been used in traditional medicine but the therapeutic potential has not been explored for cancer prevention and treatment. Current study aimed to evaluate the antioxidant and antiproliferative effects of CN, extracted in chloroform, methanol, and water, on cancer cell lines. Antioxidant properties of CN were evaluated using DPPH, galvinoxyl, nitric oxide, and hydrogen peroxide based radical scavenging assays, whereas the tumoricidal effect was tested on HepG2, IMR32, NCL-H23, SNU-1, Hela, LS-174T, K562, Raji, and IMR32 cancer cells using MTT assay. Our data showed that CN in chloroform extract was a good antioxidant against DPPH and galvinoxyl radicals, but less effective in negating nitric oxide and hydrogen peroxide radicals. Chloroform extract exerted the highest antiproliferative effect on K-562 (91.28 ± 0.03%) and Raji cell lines (88.97 ± 1.07%) at 100 μ g/ml and the other five cancer cell lines in a concentration-dependent manner, but not on IMR-32 cells. Fourteen known compounds were identified in chloroform extract, which was analysed by gas chromatography-mass spectra analysis. In conclusion, CN extracts possess antioxidant and antiproliferative properties against cultured cancer cell lines, suggesting an alternate adjunctive regimen for cancer prevention or treatment. |
| first_indexed | 2024-03-06T08:14:50Z |
| format | Article |
| id | upm.eprints-29551 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:14:50Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-295512015-12-08T05:05:07Z http://psasir.upm.edu.my/id/eprint/29551/ Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines Yong, Yoke Keong Tan, Jun Jie Teh, Soek Sin Mah, Siau Hui Ee, Gwendoline Cheng Lian Chiong, Hoe Siong Ahmad, Zuraini Clinacanthus nutans Lindau leaves (CN) have been used in traditional medicine but the therapeutic potential has not been explored for cancer prevention and treatment. Current study aimed to evaluate the antioxidant and antiproliferative effects of CN, extracted in chloroform, methanol, and water, on cancer cell lines. Antioxidant properties of CN were evaluated using DPPH, galvinoxyl, nitric oxide, and hydrogen peroxide based radical scavenging assays, whereas the tumoricidal effect was tested on HepG2, IMR32, NCL-H23, SNU-1, Hela, LS-174T, K562, Raji, and IMR32 cancer cells using MTT assay. Our data showed that CN in chloroform extract was a good antioxidant against DPPH and galvinoxyl radicals, but less effective in negating nitric oxide and hydrogen peroxide radicals. Chloroform extract exerted the highest antiproliferative effect on K-562 (91.28 ± 0.03%) and Raji cell lines (88.97 ± 1.07%) at 100 μ g/ml and the other five cancer cell lines in a concentration-dependent manner, but not on IMR-32 cells. Fourteen known compounds were identified in chloroform extract, which was analysed by gas chromatography-mass spectra analysis. In conclusion, CN extracts possess antioxidant and antiproliferative properties against cultured cancer cell lines, suggesting an alternate adjunctive regimen for cancer prevention or treatment. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29551/1/Clinacanthus%20nutans%20Extracts%20Are%20Antioxidant%20with.pdf Yong, Yoke Keong and Tan, Jun Jie and Teh, Soek Sin and Mah, Siau Hui and Ee, Gwendoline Cheng Lian and Chiong, Hoe Siong and Ahmad, Zuraini (2013) Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines. Evidence-Based Complementary and Alternative Medicine, 2013. art. no. 462751. pp. 1-8. ISSN 1741-427X; ESSN: 1741-4288 http://www.hindawi.com/journals/ecam/2013/462751/abs/ 10.1155/2013/462751 |
| spellingShingle | Yong, Yoke Keong Tan, Jun Jie Teh, Soek Sin Mah, Siau Hui Ee, Gwendoline Cheng Lian Chiong, Hoe Siong Ahmad, Zuraini Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines |
| title | Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines |
| title_full | Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines |
| title_fullStr | Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines |
| title_full_unstemmed | Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines |
| title_short | Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines |
| title_sort | clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines |
| url | http://psasir.upm.edu.my/id/eprint/29551/1/Clinacanthus%20nutans%20Extracts%20Are%20Antioxidant%20with.pdf |
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