Cytoprotective and enhanced anti-inflammatory activities of liposomal piroxicam formulation in lipopolysaccharide-stimulated RAW 264.7 macrophages
BACKGROUND: Liposomal drug delivery systems, a promising lipid-based nanoparticle technology, have been known to play significant roles in improving the safety and efficacy of an encapsulated drug. METHODS: Liposomes, prepared using an optimized proliposome method, were used in the present work to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29605/1/Cytoprotective%20and%20enhanced%20anti.pdf |
| _version_ | 1825947544288493568 |
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| author | Chiong, Hoe Siong Yong, Yoke Keong Ahmad, Zuraini Sulaiman, Mohd Roslan Zakaria, Zainul Amiruddin Yuen, Kah Hay Abdullah, Muhammad Nazrul Hakim |
| author_facet | Chiong, Hoe Siong Yong, Yoke Keong Ahmad, Zuraini Sulaiman, Mohd Roslan Zakaria, Zainul Amiruddin Yuen, Kah Hay Abdullah, Muhammad Nazrul Hakim |
| author_sort | Chiong, Hoe Siong |
| collection | UPM |
| description | BACKGROUND: Liposomal drug delivery systems, a promising lipid-based nanoparticle technology, have been known to play significant roles in improving the safety and efficacy of an encapsulated drug.
METHODS: Liposomes, prepared using an optimized proliposome method, were used in the present work to encapsulate piroxicam, a widely prescribed nonsteroidal anti-inflammatory drug. The cytotoxic effects as well as the in vitro efficacy in regulation of inflammatory responses by free-form piroxicam and liposome-encapsulated piroxicam were evaluated using a lipopolysaccharide-sensitive macrophage cell line, RAW 264.7.
RESULTS: Cells treated with liposome-encapsulated piroxicam demonstrated higher cell viabilities than those treated with free-form piroxicam. In addition, the liposomal piroxicam formulation resulted in statistically stronger inhibition of pro-inflammatory mediators (ie, nitric oxide, tumor necrosis factor-α, interleukin-1β, and prostaglandin E2) than piroxicam at an equivalent dose. The liposome-encapsulated piroxicam also caused statistically significant production of interleukin-10, an anti-inflammatory cytokine.
CONCLUSION: This study affirms the potential of a liposomal piroxicam formulation in reducing cytotoxicity and enhancing anti-inflammatory responses in vitro. |
| first_indexed | 2024-03-06T08:15:00Z |
| format | Article |
| id | upm.eprints-29605 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:15:00Z |
| publishDate | 2013 |
| publisher | Dove Medical Press |
| record_format | dspace |
| spelling | upm.eprints-296052015-09-11T01:42:15Z http://psasir.upm.edu.my/id/eprint/29605/ Cytoprotective and enhanced anti-inflammatory activities of liposomal piroxicam formulation in lipopolysaccharide-stimulated RAW 264.7 macrophages Chiong, Hoe Siong Yong, Yoke Keong Ahmad, Zuraini Sulaiman, Mohd Roslan Zakaria, Zainul Amiruddin Yuen, Kah Hay Abdullah, Muhammad Nazrul Hakim BACKGROUND: Liposomal drug delivery systems, a promising lipid-based nanoparticle technology, have been known to play significant roles in improving the safety and efficacy of an encapsulated drug. METHODS: Liposomes, prepared using an optimized proliposome method, were used in the present work to encapsulate piroxicam, a widely prescribed nonsteroidal anti-inflammatory drug. The cytotoxic effects as well as the in vitro efficacy in regulation of inflammatory responses by free-form piroxicam and liposome-encapsulated piroxicam were evaluated using a lipopolysaccharide-sensitive macrophage cell line, RAW 264.7. RESULTS: Cells treated with liposome-encapsulated piroxicam demonstrated higher cell viabilities than those treated with free-form piroxicam. In addition, the liposomal piroxicam formulation resulted in statistically stronger inhibition of pro-inflammatory mediators (ie, nitric oxide, tumor necrosis factor-α, interleukin-1β, and prostaglandin E2) than piroxicam at an equivalent dose. The liposome-encapsulated piroxicam also caused statistically significant production of interleukin-10, an anti-inflammatory cytokine. CONCLUSION: This study affirms the potential of a liposomal piroxicam formulation in reducing cytotoxicity and enhancing anti-inflammatory responses in vitro. Dove Medical Press 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29605/1/Cytoprotective%20and%20enhanced%20anti.pdf Chiong, Hoe Siong and Yong, Yoke Keong and Ahmad, Zuraini and Sulaiman, Mohd Roslan and Zakaria, Zainul Amiruddin and Yuen, Kah Hay and Abdullah, Muhammad Nazrul Hakim (2013) Cytoprotective and enhanced anti-inflammatory activities of liposomal piroxicam formulation in lipopolysaccharide-stimulated RAW 264.7 macrophages. International Journal of Nanomedicine, 8 (1). pp. 1245-1255. ISSN 1176-9114; ESSN: 1178-2013 http://www.dovepress.com/cytoprotective-and-enhanced-anti-inflammatory-activities-of-liposomal--peer-reviewed-article-IJN 10.2147/IJN.S42801 |
| spellingShingle | Chiong, Hoe Siong Yong, Yoke Keong Ahmad, Zuraini Sulaiman, Mohd Roslan Zakaria, Zainul Amiruddin Yuen, Kah Hay Abdullah, Muhammad Nazrul Hakim Cytoprotective and enhanced anti-inflammatory activities of liposomal piroxicam formulation in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title | Cytoprotective and enhanced anti-inflammatory activities of liposomal piroxicam formulation in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_full | Cytoprotective and enhanced anti-inflammatory activities of liposomal piroxicam formulation in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_fullStr | Cytoprotective and enhanced anti-inflammatory activities of liposomal piroxicam formulation in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_full_unstemmed | Cytoprotective and enhanced anti-inflammatory activities of liposomal piroxicam formulation in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_short | Cytoprotective and enhanced anti-inflammatory activities of liposomal piroxicam formulation in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_sort | cytoprotective and enhanced anti inflammatory activities of liposomal piroxicam formulation in lipopolysaccharide stimulated raw 264 7 macrophages |
| url | http://psasir.upm.edu.my/id/eprint/29605/1/Cytoprotective%20and%20enhanced%20anti.pdf |
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