Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia
We developed a time-saving and cost-efficient Pulsed Field Gel Electrophoresis (PFGE) method for the typing of Stenotrophomonas maltophilia by modifying the conventional procedures. Our modifications related to the cell suspension preparation, lysis of bacterial cells in plugs, washing steps, and co...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29607/1/Simple.pdf |
| _version_ | 1796971573627846656 |
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| author | Chong, Seng Shueh Neela, Vasanthakumari Hussin, Salasawati Awang Hamat, Rukman |
| author_facet | Chong, Seng Shueh Neela, Vasanthakumari Hussin, Salasawati Awang Hamat, Rukman |
| author_sort | Chong, Seng Shueh |
| collection | UPM |
| description | We developed a time-saving and cost-efficient Pulsed Field Gel Electrophoresis (PFGE) method for the typing of Stenotrophomonas maltophilia by modifying the conventional procedures. Our modifications related to the cell suspension preparation, lysis of bacterial cells in plugs, washing steps, and consumption of restriction enzyme. Although few rapid PFGE protocols on Gram-negative bacteria are available, the use of comparatively large amounts of costly reagents prompted us to look for other alternative. Hence, by considering the speed, simplicity, and relatively low cost, the modified protocol may be of more practical value than other established protocols in investigating S. maltophilia nosocomial outbreaks. |
| first_indexed | 2024-03-06T08:15:00Z |
| format | Article |
| id | upm.eprints-29607 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:15:00Z |
| publishDate | 2013 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | upm.eprints-296072015-09-08T04:27:52Z http://psasir.upm.edu.my/id/eprint/29607/ Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia Chong, Seng Shueh Neela, Vasanthakumari Hussin, Salasawati Awang Hamat, Rukman We developed a time-saving and cost-efficient Pulsed Field Gel Electrophoresis (PFGE) method for the typing of Stenotrophomonas maltophilia by modifying the conventional procedures. Our modifications related to the cell suspension preparation, lysis of bacterial cells in plugs, washing steps, and consumption of restriction enzyme. Although few rapid PFGE protocols on Gram-negative bacteria are available, the use of comparatively large amounts of costly reagents prompted us to look for other alternative. Hence, by considering the speed, simplicity, and relatively low cost, the modified protocol may be of more practical value than other established protocols in investigating S. maltophilia nosocomial outbreaks. Elsevier BV 2013-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29607/1/Simple.pdf Chong, Seng Shueh and Neela, Vasanthakumari and Hussin, Salasawati and Awang Hamat, Rukman (2013) Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia. Journal of Microbiological Methods, 94 (2). pp. 141-143. ISSN 0167-7012; ESSN: 1872-8359 10.1016/j.mimet.2013.06.001. |
| spellingShingle | Chong, Seng Shueh Neela, Vasanthakumari Hussin, Salasawati Awang Hamat, Rukman Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia |
| title | Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia |
| title_full | Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia |
| title_fullStr | Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia |
| title_full_unstemmed | Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia |
| title_short | Simple, time saving pulsed-field gel electrophoresis protocol for the typing of Stenotrophomonas maltophilia |
| title_sort | simple time saving pulsed field gel electrophoresis protocol for the typing of stenotrophomonas maltophilia |
| url | http://psasir.upm.edu.my/id/eprint/29607/1/Simple.pdf |
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