Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle
Mesenchymal stem cells (MSC) generated from human umbilical cord (UC-MSC) and placenta (PLC-MSC) were assessed and compared for their immunomodulatory function on T cells proliferation by analysis of the cell cycle. Mitogen stimulated or resting T cells were co-cultured in the presence or absence of...
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| Format: | Article |
| Language: | English |
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International Federation for Cell Biology
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29676/1/Mesenchymal%20stem%20cells%20of%20human%20placenta%20and%20umbilical%20cord%20suppress%20T.pdf |
| _version_ | 1796971587708125184 |
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| author | Vellasamy, Shalini Sandrasaigaran, Pratheep Vidyadaran, Sharmili Abdullah, Maha George, Elizabeth Ramasamy, Rajesh |
| author_facet | Vellasamy, Shalini Sandrasaigaran, Pratheep Vidyadaran, Sharmili Abdullah, Maha George, Elizabeth Ramasamy, Rajesh |
| author_sort | Vellasamy, Shalini |
| collection | UPM |
| description | Mesenchymal stem cells (MSC) generated from human umbilical cord (UC-MSC) and placenta (PLC-MSC) were assessed and compared for their immunomodulatory function on T cells proliferation by analysis of the cell cycle. Mitogen stimulated or resting T cells were co-cultured in the presence or absence of MSC. T-cell proliferation was assessed by tritiated thymidine ((3) H-TdR) assay and the mechanism of inhibition was examined bycell cycle and apoptosis assay. Both UC-MSC and PLC-MSC profoundly inhibited the proliferation of T-cell, mainly via cell-to-cell contact. MSC-mediated anti-proliferation does not lead to apoptosis,but prevented T cells from entering S phase and they therefore accumulated in the G(0) /G(1) phases. The anti-proliferative activity of MSC was related to this cell cycle arrest of T-cell. UC-MSC produced a greater inhibition than PLC-MSC in all measured parameters. |
| first_indexed | 2024-03-06T08:15:13Z |
| format | Article |
| id | upm.eprints-29676 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:15:13Z |
| publishDate | 2013 |
| publisher | International Federation for Cell Biology |
| record_format | dspace |
| spelling | upm.eprints-296762016-02-03T02:24:16Z http://psasir.upm.edu.my/id/eprint/29676/ Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle Vellasamy, Shalini Sandrasaigaran, Pratheep Vidyadaran, Sharmili Abdullah, Maha George, Elizabeth Ramasamy, Rajesh Mesenchymal stem cells (MSC) generated from human umbilical cord (UC-MSC) and placenta (PLC-MSC) were assessed and compared for their immunomodulatory function on T cells proliferation by analysis of the cell cycle. Mitogen stimulated or resting T cells were co-cultured in the presence or absence of MSC. T-cell proliferation was assessed by tritiated thymidine ((3) H-TdR) assay and the mechanism of inhibition was examined bycell cycle and apoptosis assay. Both UC-MSC and PLC-MSC profoundly inhibited the proliferation of T-cell, mainly via cell-to-cell contact. MSC-mediated anti-proliferation does not lead to apoptosis,but prevented T cells from entering S phase and they therefore accumulated in the G(0) /G(1) phases. The anti-proliferative activity of MSC was related to this cell cycle arrest of T-cell. UC-MSC produced a greater inhibition than PLC-MSC in all measured parameters. International Federation for Cell Biology 2013-03-30 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29676/1/Mesenchymal%20stem%20cells%20of%20human%20placenta%20and%20umbilical%20cord%20suppress%20T.pdf Vellasamy, Shalini and Sandrasaigaran, Pratheep and Vidyadaran, Sharmili and Abdullah, Maha and George, Elizabeth and Ramasamy, Rajesh (2013) Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle. Cell Biology International, 37 (3). pp. 250-256. ISSN 1065-6995; ESSN: 1095-8355 10.1002/cbin.10033 |
| spellingShingle | Vellasamy, Shalini Sandrasaigaran, Pratheep Vidyadaran, Sharmili Abdullah, Maha George, Elizabeth Ramasamy, Rajesh Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle |
| title | Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle |
| title_full | Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle |
| title_fullStr | Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle |
| title_full_unstemmed | Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle |
| title_short | Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G0 phase of cell cycle |
| title_sort | mesenchymal stem cells of human placenta and umbilical cord suppress t cell proliferation at g0 phase of cell cycle |
| url | http://psasir.upm.edu.my/id/eprint/29676/1/Mesenchymal%20stem%20cells%20of%20human%20placenta%20and%20umbilical%20cord%20suppress%20T.pdf |
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