Tamoxifen-loaded nanostructured lipid carrier as a drug delivery system: characterization, stability assessment and cytotoxicity
Cancer nanotherapeutics is beginning to overwhelm the global research and viewed to be the revolutionary treatment regime in the medical field. This investigation describes the development of a stable nanostructured lipid carrier (NLC) system as carrier for Tamoxifen (TAM). The TAM-loaded NLC (TAM-N...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29683/1/Tamoxifen.pdf |
| _version_ | 1825947559342899200 |
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| author | How, Chee Wun Rasedee, Abdullah Manickam, Sivakumar Rosli, Rozita |
| author_facet | How, Chee Wun Rasedee, Abdullah Manickam, Sivakumar Rosli, Rozita |
| author_sort | How, Chee Wun |
| collection | UPM |
| description | Cancer nanotherapeutics is beginning to overwhelm the global research and viewed to be the revolutionary treatment regime in the medical field. This investigation describes the development of a stable nanostructured lipid carrier (NLC) system as carrier for Tamoxifen (TAM). The TAM-loaded NLC (TAM-NLC) developed with 200 mg of TAM showed a spherical particle with the size of 46.6 nm, polydispersity index of 0.267, entrapment efficiency of 99.74% and with the zeta potential of -23.78 mV. Besides, the equivalent cytotoxicity of TAM and TAM-NLC to human (MCF-7) and mice (4T1) mammary breast cancer cell lines were observed. Incubating the formulation at the physiological pH resulted into reduced Ostwald ripening rate but without any significant change in the absorptivity. When coupled with the measurements of zeta potential and Ostwald ripening rate, the absorbance assay may be used to predict the long-term stability of drug-loaded nanoparticle formulations. The results of the study also suggest that TAM-NLC is a promising drug delivery system for breast cancer therapy. This is the first encouraging report on the in vitro effect of TAM-NLC against human and mouse mammary adenocarcinoma cell lines. |
| first_indexed | 2024-03-06T08:15:14Z |
| format | Article |
| id | upm.eprints-29683 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:15:14Z |
| publishDate | 2013 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | upm.eprints-296832015-09-10T07:10:17Z http://psasir.upm.edu.my/id/eprint/29683/ Tamoxifen-loaded nanostructured lipid carrier as a drug delivery system: characterization, stability assessment and cytotoxicity How, Chee Wun Rasedee, Abdullah Manickam, Sivakumar Rosli, Rozita Cancer nanotherapeutics is beginning to overwhelm the global research and viewed to be the revolutionary treatment regime in the medical field. This investigation describes the development of a stable nanostructured lipid carrier (NLC) system as carrier for Tamoxifen (TAM). The TAM-loaded NLC (TAM-NLC) developed with 200 mg of TAM showed a spherical particle with the size of 46.6 nm, polydispersity index of 0.267, entrapment efficiency of 99.74% and with the zeta potential of -23.78 mV. Besides, the equivalent cytotoxicity of TAM and TAM-NLC to human (MCF-7) and mice (4T1) mammary breast cancer cell lines were observed. Incubating the formulation at the physiological pH resulted into reduced Ostwald ripening rate but without any significant change in the absorptivity. When coupled with the measurements of zeta potential and Ostwald ripening rate, the absorbance assay may be used to predict the long-term stability of drug-loaded nanoparticle formulations. The results of the study also suggest that TAM-NLC is a promising drug delivery system for breast cancer therapy. This is the first encouraging report on the in vitro effect of TAM-NLC against human and mouse mammary adenocarcinoma cell lines. Elsevier BV 2013-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29683/1/Tamoxifen.pdf How, Chee Wun and Rasedee, Abdullah and Manickam, Sivakumar and Rosli, Rozita (2013) Tamoxifen-loaded nanostructured lipid carrier as a drug delivery system: characterization, stability assessment and cytotoxicity. Colloids and Surfaces B: Biointerfaces, 112 (1). pp. 393-399. ISSN 0927-7765; ESSN: 1873-4367 10.1016/j.colsurfb.2013.08.009 |
| spellingShingle | How, Chee Wun Rasedee, Abdullah Manickam, Sivakumar Rosli, Rozita Tamoxifen-loaded nanostructured lipid carrier as a drug delivery system: characterization, stability assessment and cytotoxicity |
| title | Tamoxifen-loaded nanostructured lipid carrier as a drug delivery system: characterization, stability assessment and cytotoxicity |
| title_full | Tamoxifen-loaded nanostructured lipid carrier as a drug delivery system: characterization, stability assessment and cytotoxicity |
| title_fullStr | Tamoxifen-loaded nanostructured lipid carrier as a drug delivery system: characterization, stability assessment and cytotoxicity |
| title_full_unstemmed | Tamoxifen-loaded nanostructured lipid carrier as a drug delivery system: characterization, stability assessment and cytotoxicity |
| title_short | Tamoxifen-loaded nanostructured lipid carrier as a drug delivery system: characterization, stability assessment and cytotoxicity |
| title_sort | tamoxifen loaded nanostructured lipid carrier as a drug delivery system characterization stability assessment and cytotoxicity |
| url | http://psasir.upm.edu.my/id/eprint/29683/1/Tamoxifen.pdf |
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