Safety profile of dextran-spermine gene delivery vector in mouse lungs
A nano-sized polymer, dextran-spermine (D-SPM), was shown to have the capacity to deliver gene to the lung of mouse via intranasal route. In this study, assessments on the safety profile of D-SPM were performed to complement the gene expression results. African green monkey kidney fibroblast (COS-7)...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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American Scientific Publishers
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29784/1/Safety%20profile%20of%20dextran%20spermine%20gene%20delivery%20vector%20in%20mouse%20lungs.pdf |
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| author | Yeo, Wendy Wai Yeng Hosseinkhani, Hossein Abdul Rahman, Sabariah Rosli, Rozita Domb, Abraham J. Abdullah, Syahrilnizam |
| author_facet | Yeo, Wendy Wai Yeng Hosseinkhani, Hossein Abdul Rahman, Sabariah Rosli, Rozita Domb, Abraham J. Abdullah, Syahrilnizam |
| author_sort | Yeo, Wendy Wai Yeng |
| collection | UPM |
| description | A nano-sized polymer, dextran-spermine (D-SPM), was shown to have the capacity to deliver gene to the lung of mouse via intranasal route. In this study, assessments on the safety profile of D-SPM were performed to complement the gene expression results. African green monkey kidney fibroblast (COS-7) and human adenocarcinoma breast (MCF-7) cells transfected with D-SPM/pDNA showed massive reduction in the number of viable cells. As for in vivo study, elevated level of neutrophils was observed, despite the minimal level of pro-inflammatory cytokines (TNF-α, IL-12, IFN-γ detected in the bronchoalveolar lavage fluid (BALF) of mice treated with the D-SPM/pDNA complexes. Histology profile examinations of the lungs showed mild inflammatory responses, with inflamed areas overlap with healthy areas. Although reduction of mice weight was seen at day 1 post administration, the mice did not show any sign of abnormal behavior or physical appearance. Biodistribution study was performed to determine the ability of the D-SPM/pDNA complexes to infiltrate to other non-intended organs. The result showed that the D-SPM/pDNA complexes were only localized at the lung and no gene expression was detected in other organs or blood. In short, these results indicate that the D-SPM/pDNA exhibited mild toxicity in the mouse lungs. |
| first_indexed | 2024-03-06T08:15:32Z |
| format | Article |
| id | upm.eprints-29784 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:15:32Z |
| publishDate | 2014 |
| publisher | American Scientific Publishers |
| record_format | dspace |
| spelling | upm.eprints-297842015-10-22T03:34:57Z http://psasir.upm.edu.my/id/eprint/29784/ Safety profile of dextran-spermine gene delivery vector in mouse lungs Yeo, Wendy Wai Yeng Hosseinkhani, Hossein Abdul Rahman, Sabariah Rosli, Rozita Domb, Abraham J. Abdullah, Syahrilnizam A nano-sized polymer, dextran-spermine (D-SPM), was shown to have the capacity to deliver gene to the lung of mouse via intranasal route. In this study, assessments on the safety profile of D-SPM were performed to complement the gene expression results. African green monkey kidney fibroblast (COS-7) and human adenocarcinoma breast (MCF-7) cells transfected with D-SPM/pDNA showed massive reduction in the number of viable cells. As for in vivo study, elevated level of neutrophils was observed, despite the minimal level of pro-inflammatory cytokines (TNF-α, IL-12, IFN-γ detected in the bronchoalveolar lavage fluid (BALF) of mice treated with the D-SPM/pDNA complexes. Histology profile examinations of the lungs showed mild inflammatory responses, with inflamed areas overlap with healthy areas. Although reduction of mice weight was seen at day 1 post administration, the mice did not show any sign of abnormal behavior or physical appearance. Biodistribution study was performed to determine the ability of the D-SPM/pDNA complexes to infiltrate to other non-intended organs. The result showed that the D-SPM/pDNA complexes were only localized at the lung and no gene expression was detected in other organs or blood. In short, these results indicate that the D-SPM/pDNA exhibited mild toxicity in the mouse lungs. American Scientific Publishers 2014-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29784/1/Safety%20profile%20of%20dextran%20spermine%20gene%20delivery%20vector%20in%20mouse%20lungs.pdf Yeo, Wendy Wai Yeng and Hosseinkhani, Hossein and Abdul Rahman, Sabariah and Rosli, Rozita and Domb, Abraham J. and Abdullah, Syahrilnizam (2014) Safety profile of dextran-spermine gene delivery vector in mouse lungs. Journal of Nanoscience and Nanotechnology , 14 (5). pp. 3328-3336. ISSN 1533-4880;ESSN: 1533-4899 |
| spellingShingle | Yeo, Wendy Wai Yeng Hosseinkhani, Hossein Abdul Rahman, Sabariah Rosli, Rozita Domb, Abraham J. Abdullah, Syahrilnizam Safety profile of dextran-spermine gene delivery vector in mouse lungs |
| title | Safety profile of dextran-spermine gene delivery vector in mouse lungs |
| title_full | Safety profile of dextran-spermine gene delivery vector in mouse lungs |
| title_fullStr | Safety profile of dextran-spermine gene delivery vector in mouse lungs |
| title_full_unstemmed | Safety profile of dextran-spermine gene delivery vector in mouse lungs |
| title_short | Safety profile of dextran-spermine gene delivery vector in mouse lungs |
| title_sort | safety profile of dextran spermine gene delivery vector in mouse lungs |
| url | http://psasir.upm.edu.my/id/eprint/29784/1/Safety%20profile%20of%20dextran%20spermine%20gene%20delivery%20vector%20in%20mouse%20lungs.pdf |
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