Genotypically different clones of Staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations
This study evaluated whether genotypically different clinical isolates of S. aureus have similar susceptibilities to individual antibiotics. It further aims to check the impact of biofilm on the in vitro activity of vancomycin, daptomycin, linezolid, and tigecycline against S. aureus clones. The stu...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29793/1/Genotypically%20different%20clones%20of%20Staphylococcus%20aureus%20are%20diverse%20in%20the%20antimicrobial%20susceptibility%20patterns%20and%20biofilm%20formations.pdf |
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| author | Atshan, Salman Sahab Shamsudin, Mariana Nor Than, Leslie Thian Lung Sekawi, Zamberi Chong, Pei Pei Karunanidhi, Arunkumar Jeevajothi Nathan, Jayakayatri Mateg Ali, Alreshidi Ghaznavi-Rad, Ehsanollah Abduljaleel, Salwa Awang Hamat, Rukman |
| author_facet | Atshan, Salman Sahab Shamsudin, Mariana Nor Than, Leslie Thian Lung Sekawi, Zamberi Chong, Pei Pei Karunanidhi, Arunkumar Jeevajothi Nathan, Jayakayatri Mateg Ali, Alreshidi Ghaznavi-Rad, Ehsanollah Abduljaleel, Salwa Awang Hamat, Rukman |
| author_sort | Atshan, Salman Sahab |
| collection | UPM |
| description | This study evaluated whether genotypically different clinical isolates of S. aureus have similar susceptibilities to individual antibiotics. It further aims to check the impact of biofilm on the in vitro activity of vancomycin, daptomycin, linezolid, and tigecycline against S. aureus clones. The study used a total of 60 different clinical MSSA and MRSA isolates. Susceptibilities were performed in planktonic cultures by macrobroth dilution and epsilon-test (E test) system. Biofilm production was determined using an adherent plate assay. The efficacy of antimicrobial activities against biofilms formation was checked using confocal laser scanning microscopy (CLSM). The study found that similar and different spa, MLST, and SCCmec types displayed high variation in their susceptibilities to antibiotics with tigecycline and daptomycin being the most effective. The biofilms were found resistant to high concentrations of most antibiotics tested with daptomycin being the most effective drug used in adhesive biofilms. A considerable difference exists among similar and various clone types against antibiotics tested. This variation could have contributed to the degree of virulence even within the same clonal genotype and enhanced heterogeneity in the infection potential. Thus, the development of a rapid and precise identification profile for each clone in human infections is important. |
| first_indexed | 2024-03-06T08:15:34Z |
| format | Article |
| id | upm.eprints-29793 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:15:34Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-297932016-02-12T04:22:55Z http://psasir.upm.edu.my/id/eprint/29793/ Genotypically different clones of Staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations Atshan, Salman Sahab Shamsudin, Mariana Nor Than, Leslie Thian Lung Sekawi, Zamberi Chong, Pei Pei Karunanidhi, Arunkumar Jeevajothi Nathan, Jayakayatri Mateg Ali, Alreshidi Ghaznavi-Rad, Ehsanollah Abduljaleel, Salwa Awang Hamat, Rukman This study evaluated whether genotypically different clinical isolates of S. aureus have similar susceptibilities to individual antibiotics. It further aims to check the impact of biofilm on the in vitro activity of vancomycin, daptomycin, linezolid, and tigecycline against S. aureus clones. The study used a total of 60 different clinical MSSA and MRSA isolates. Susceptibilities were performed in planktonic cultures by macrobroth dilution and epsilon-test (E test) system. Biofilm production was determined using an adherent plate assay. The efficacy of antimicrobial activities against biofilms formation was checked using confocal laser scanning microscopy (CLSM). The study found that similar and different spa, MLST, and SCCmec types displayed high variation in their susceptibilities to antibiotics with tigecycline and daptomycin being the most effective. The biofilms were found resistant to high concentrations of most antibiotics tested with daptomycin being the most effective drug used in adhesive biofilms. A considerable difference exists among similar and various clone types against antibiotics tested. This variation could have contributed to the degree of virulence even within the same clonal genotype and enhanced heterogeneity in the infection potential. Thus, the development of a rapid and precise identification profile for each clone in human infections is important. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29793/1/Genotypically%20different%20clones%20of%20Staphylococcus%20aureus%20are%20diverse%20in%20the%20antimicrobial%20susceptibility%20patterns%20and%20biofilm%20formations.pdf Atshan, Salman Sahab and Shamsudin, Mariana Nor and Than, Leslie Thian Lung and Sekawi, Zamberi and Chong, Pei Pei and Karunanidhi, Arunkumar and Jeevajothi Nathan, Jayakayatri and Mateg Ali, Alreshidi and Ghaznavi-Rad, Ehsanollah and Abduljaleel, Salwa and Awang Hamat, Rukman (2013) Genotypically different clones of Staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations. BioMed Research International, 2013. art. no. 515712. pp. 1-10. ISSN 2314-6133; ESSN: 2314-6141 http://dx.doi.org/10.1155/2013/515712 10.1155/2013/515712 |
| spellingShingle | Atshan, Salman Sahab Shamsudin, Mariana Nor Than, Leslie Thian Lung Sekawi, Zamberi Chong, Pei Pei Karunanidhi, Arunkumar Jeevajothi Nathan, Jayakayatri Mateg Ali, Alreshidi Ghaznavi-Rad, Ehsanollah Abduljaleel, Salwa Awang Hamat, Rukman Genotypically different clones of Staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations |
| title | Genotypically different clones of Staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations |
| title_full | Genotypically different clones of Staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations |
| title_fullStr | Genotypically different clones of Staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations |
| title_full_unstemmed | Genotypically different clones of Staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations |
| title_short | Genotypically different clones of Staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations |
| title_sort | genotypically different clones of staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations |
| url | http://psasir.upm.edu.my/id/eprint/29793/1/Genotypically%20different%20clones%20of%20Staphylococcus%20aureus%20are%20diverse%20in%20the%20antimicrobial%20susceptibility%20patterns%20and%20biofilm%20formations.pdf |
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