Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection
Fifteen sequences with stop codons have been obtained in the course of standard methicillin-resistant Staphylococcus aureus (MRSA) spa typing. In nine of those sequences, stop codons occurred due to nonsense G-T and A-T transversions. G-T transversions would appear to be frequent in the spa gene, mo...
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| Format: | Article |
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Elsevier
2013
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| _version_ | 1825947582860361728 |
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| author | Khrustalev, Vladislav Victorovich Ghaznavi Rad, Ehsanollah Neela, Vasantha Kumari Shamsudin, Mariana Nor Amouzandeh-Nobaveh, Alireza Barkovsky, Eugene Victorovich |
| author_facet | Khrustalev, Vladislav Victorovich Ghaznavi Rad, Ehsanollah Neela, Vasantha Kumari Shamsudin, Mariana Nor Amouzandeh-Nobaveh, Alireza Barkovsky, Eugene Victorovich |
| author_sort | Khrustalev, Vladislav Victorovich |
| collection | UPM |
| description | Fifteen sequences with stop codons have been obtained in the course of standard methicillin-resistant Staphylococcus aureus (MRSA) spa typing. In nine of those sequences, stop codons occurred due to nonsense G-T and A-T transversions. G-T transversions would appear to be frequent in the spa gene, mostly due to symmetric mutational AT-pressure in the whole S. aureus genome and due to replication-associated mutational pressure characteristic of lagging strands of the "chromosome". A-T transversions would appear to be frequent in the spa gene mostly due to transcription-associated mutational pressure. Relative to other S. aureus genes, short repeats in spa are enriched by nonsense sites for G-T and A-T transversions; the probability of being nonsense for A-T transversion is high in that part of spa coding region. 13 out of 15 (87%) of the sequences with stop codons were obtained from strains isolated from patients with generalized S. aureus infection. Truncation of spa at its C-terminus is predicted to result in a protein that possesses functional IgG binding domains unable to be linked to the cell wall. This is discussed in light of the known fact that extracellular spa is a strong virulence factor involved in immune evasion. |
| first_indexed | 2024-03-06T08:15:36Z |
| format | Article |
| id | upm.eprints-29802 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:15:36Z |
| publishDate | 2013 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-298022016-02-19T02:24:16Z http://psasir.upm.edu.my/id/eprint/29802/ Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection Khrustalev, Vladislav Victorovich Ghaznavi Rad, Ehsanollah Neela, Vasantha Kumari Shamsudin, Mariana Nor Amouzandeh-Nobaveh, Alireza Barkovsky, Eugene Victorovich Fifteen sequences with stop codons have been obtained in the course of standard methicillin-resistant Staphylococcus aureus (MRSA) spa typing. In nine of those sequences, stop codons occurred due to nonsense G-T and A-T transversions. G-T transversions would appear to be frequent in the spa gene, mostly due to symmetric mutational AT-pressure in the whole S. aureus genome and due to replication-associated mutational pressure characteristic of lagging strands of the "chromosome". A-T transversions would appear to be frequent in the spa gene mostly due to transcription-associated mutational pressure. Relative to other S. aureus genes, short repeats in spa are enriched by nonsense sites for G-T and A-T transversions; the probability of being nonsense for A-T transversion is high in that part of spa coding region. 13 out of 15 (87%) of the sequences with stop codons were obtained from strains isolated from patients with generalized S. aureus infection. Truncation of spa at its C-terminus is predicted to result in a protein that possesses functional IgG binding domains unable to be linked to the cell wall. This is discussed in light of the known fact that extracellular spa is a strong virulence factor involved in immune evasion. Elsevier 2013-11 Article PeerReviewed Khrustalev, Vladislav Victorovich and Ghaznavi Rad, Ehsanollah and Neela, Vasantha Kumari and Shamsudin, Mariana Nor and Amouzandeh-Nobaveh, Alireza and Barkovsky, Eugene Victorovich (2013) Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection. Research in Microbiology, 164 (9). pp. 913-922. ISSN 0923-2508; ESSN: 1769-7123 10.1016/j.resmic.2013.07.003 |
| spellingShingle | Khrustalev, Vladislav Victorovich Ghaznavi Rad, Ehsanollah Neela, Vasantha Kumari Shamsudin, Mariana Nor Amouzandeh-Nobaveh, Alireza Barkovsky, Eugene Victorovich Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection |
| title | Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection |
| title_full | Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection |
| title_fullStr | Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection |
| title_full_unstemmed | Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection |
| title_short | Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection |
| title_sort | short repeats in the spa gene of staphylococcus aureus are prone to nonsense mutations stop codons can be found in strains isolated from patients with generalized infection |
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