Mucosal genetic immunization through microsphere-based oral carriers.
Polymeric carriers in the form of cellulose acetate phthalate (CAP) and alginate (ALG) microspheres were used for encapsulation of plasmid DNA for oral mucosal immunization. Access into the intestinal mucosa by pVAXI eukaryotic expression plasmid vectors carrying gene-coding sequences, either for th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Landes Bioscience
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29817/1/Mucosal%20genetic%20immunization%20through%20microsphere.pdf |
| _version_ | 1796971616666648576 |
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| author | Rosli, Rozita Nograles, Nadine Hanafi, Aimi Melati Shamsudin, Mariana Nor Abdullah, Syahrilnizam |
| author_facet | Rosli, Rozita Nograles, Nadine Hanafi, Aimi Melati Shamsudin, Mariana Nor Abdullah, Syahrilnizam |
| author_sort | Rosli, Rozita |
| collection | UPM |
| description | Polymeric carriers in the form of cellulose acetate phthalate (CAP) and alginate (ALG) microspheres were used for encapsulation of plasmid DNA for oral mucosal immunization. Access into the intestinal mucosa by pVAXI eukaryotic expression plasmid vectors carrying gene-coding sequences, either for the cholera enterotoxin B subunit (cfxB) immunostimulatory antigen or the green fluorescent protein (GFP), delivered from both types of microsphere carriers were examined in orally immunized BALB/c mice. Demonstration of transgene protein expression and IgA antibody responses at local mucosal sites suggest immunological response to a potential oral DNA vaccine formulated within the microsphere carriers. |
| first_indexed | 2024-03-06T08:15:38Z |
| format | Article |
| id | upm.eprints-29817 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:15:38Z |
| publishDate | 2013 |
| publisher | Landes Bioscience |
| record_format | dspace |
| spelling | upm.eprints-298172015-10-29T08:15:56Z http://psasir.upm.edu.my/id/eprint/29817/ Mucosal genetic immunization through microsphere-based oral carriers. Rosli, Rozita Nograles, Nadine Hanafi, Aimi Melati Shamsudin, Mariana Nor Abdullah, Syahrilnizam Polymeric carriers in the form of cellulose acetate phthalate (CAP) and alginate (ALG) microspheres were used for encapsulation of plasmid DNA for oral mucosal immunization. Access into the intestinal mucosa by pVAXI eukaryotic expression plasmid vectors carrying gene-coding sequences, either for the cholera enterotoxin B subunit (cfxB) immunostimulatory antigen or the green fluorescent protein (GFP), delivered from both types of microsphere carriers were examined in orally immunized BALB/c mice. Demonstration of transgene protein expression and IgA antibody responses at local mucosal sites suggest immunological response to a potential oral DNA vaccine formulated within the microsphere carriers. Landes Bioscience 2013-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29817/1/Mucosal%20genetic%20immunization%20through%20microsphere.pdf Rosli, Rozita and Nograles, Nadine and Hanafi, Aimi Melati and Shamsudin, Mariana Nor and Abdullah, Syahrilnizam (2013) Mucosal genetic immunization through microsphere-based oral carriers. Human Vaccines and Immunotherapeutics, 9 (10). pp. 2222-2227. ISSN 2164-5515; ESSN: 2164-554X 10.4161/hv.25325 |
| spellingShingle | Rosli, Rozita Nograles, Nadine Hanafi, Aimi Melati Shamsudin, Mariana Nor Abdullah, Syahrilnizam Mucosal genetic immunization through microsphere-based oral carriers. |
| title | Mucosal genetic immunization through microsphere-based oral carriers. |
| title_full | Mucosal genetic immunization through microsphere-based oral carriers. |
| title_fullStr | Mucosal genetic immunization through microsphere-based oral carriers. |
| title_full_unstemmed | Mucosal genetic immunization through microsphere-based oral carriers. |
| title_short | Mucosal genetic immunization through microsphere-based oral carriers. |
| title_sort | mucosal genetic immunization through microsphere based oral carriers |
| url | http://psasir.upm.edu.my/id/eprint/29817/1/Mucosal%20genetic%20immunization%20through%20microsphere.pdf |
| work_keys_str_mv | AT roslirozita mucosalgeneticimmunizationthroughmicrospherebasedoralcarriers AT nogralesnadine mucosalgeneticimmunizationthroughmicrospherebasedoralcarriers AT hanafiaimimelati mucosalgeneticimmunizationthroughmicrospherebasedoralcarriers AT shamsudinmariananor mucosalgeneticimmunizationthroughmicrospherebasedoralcarriers AT abdullahsyahrilnizam mucosalgeneticimmunizationthroughmicrospherebasedoralcarriers |