Occurrence of non-O157 Shiga toxin-producing Escherichia coli in healthy cattle and goats and distribution of virulence genes among isolates
Shiga toxin-producing Escherichia coli (STEC) are receiving more attention mainly because they are zoonotic and food borne in nature. The objectives of the present study were to determine the occurrence of non-O157 Shiga toxin producing E. coli in cattle and goats and distribution of the virulence...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29850/1/29850.pdf |
| _version_ | 1825947592098316288 |
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| author | Tayzar, A. C. Abdul Aziz, Saleha Abdul Mutalib, Abdul Rahim Marimuthu, Murugaiyah Shah, Atta Hussain |
| author_facet | Tayzar, A. C. Abdul Aziz, Saleha Abdul Mutalib, Abdul Rahim Marimuthu, Murugaiyah Shah, Atta Hussain |
| author_sort | Tayzar, A. C. |
| collection | UPM |
| description | Shiga toxin-producing Escherichia coli (STEC) are receiving more attention mainly because they are zoonotic and food borne in nature. The objectives of the present study were to determine the occurrence of non-O157 Shiga toxin producing E. coli in cattle and goats and distribution of the virulence genes in the isolates. The overall isolation rates of non-O157 STEC was 15.27% (7/87) in cattle and 8.14% (22/144) in goats. Four serogroups namely O8, O26, O103 and O128 were detected. Of these serogroups, O8, O26 and O103 were common in both, cattle and goats, whereas O128 was carried by goats only. Using Duopath Verotoxin (DV) test, 52.63% of sero-positive E. coli isolates from cattle were positive for Stx1 shiga toxin whereas none of the isolates was positive for Stx2. Similarly, 42.9% (3/7) of sero-positive E. coli isolates from goats produced Stx1 and 14.3% (1/7) were positive for both, Stx1 and Stx2. The study on virulence genes showed that stx1 was commonly distributed among non-O157 STEC isolates of cattle (57.9%) and goats (57.1%). |
| first_indexed | 2024-03-06T08:15:44Z |
| format | Article |
| id | upm.eprints-29850 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:15:44Z |
| publishDate | 2013 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-298502017-11-20T02:18:15Z http://psasir.upm.edu.my/id/eprint/29850/ Occurrence of non-O157 Shiga toxin-producing Escherichia coli in healthy cattle and goats and distribution of virulence genes among isolates Tayzar, A. C. Abdul Aziz, Saleha Abdul Mutalib, Abdul Rahim Marimuthu, Murugaiyah Shah, Atta Hussain Shiga toxin-producing Escherichia coli (STEC) are receiving more attention mainly because they are zoonotic and food borne in nature. The objectives of the present study were to determine the occurrence of non-O157 Shiga toxin producing E. coli in cattle and goats and distribution of the virulence genes in the isolates. The overall isolation rates of non-O157 STEC was 15.27% (7/87) in cattle and 8.14% (22/144) in goats. Four serogroups namely O8, O26, O103 and O128 were detected. Of these serogroups, O8, O26 and O103 were common in both, cattle and goats, whereas O128 was carried by goats only. Using Duopath Verotoxin (DV) test, 52.63% of sero-positive E. coli isolates from cattle were positive for Stx1 shiga toxin whereas none of the isolates was positive for Stx2. Similarly, 42.9% (3/7) of sero-positive E. coli isolates from goats produced Stx1 and 14.3% (1/7) were positive for both, Stx1 and Stx2. The study on virulence genes showed that stx1 was commonly distributed among non-O157 STEC isolates of cattle (57.9%) and goats (57.1%). Academic Journals 2013-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29850/1/29850.pdf Tayzar, A. C. and Abdul Aziz, Saleha and Abdul Mutalib, Abdul Rahim and Marimuthu, Murugaiyah and Shah, Atta Hussain (2013) Occurrence of non-O157 Shiga toxin-producing Escherichia coli in healthy cattle and goats and distribution of virulence genes among isolates. African Journal of Microbiology Research, 7 (17). art. no. 2ABF95917095. pp. 1703-1707. ISSN 1996-0808 http://www.academicjournals.org/journal/AJMR/article-abstract/2ABF95917095 |
| spellingShingle | Tayzar, A. C. Abdul Aziz, Saleha Abdul Mutalib, Abdul Rahim Marimuthu, Murugaiyah Shah, Atta Hussain Occurrence of non-O157 Shiga toxin-producing Escherichia coli in healthy cattle and goats and distribution of virulence genes among isolates |
| title | Occurrence of non-O157 Shiga toxin-producing Escherichia coli in healthy cattle and goats and distribution of virulence genes among isolates |
| title_full | Occurrence of non-O157 Shiga toxin-producing Escherichia coli in healthy cattle and goats and distribution of virulence genes among isolates |
| title_fullStr | Occurrence of non-O157 Shiga toxin-producing Escherichia coli in healthy cattle and goats and distribution of virulence genes among isolates |
| title_full_unstemmed | Occurrence of non-O157 Shiga toxin-producing Escherichia coli in healthy cattle and goats and distribution of virulence genes among isolates |
| title_short | Occurrence of non-O157 Shiga toxin-producing Escherichia coli in healthy cattle and goats and distribution of virulence genes among isolates |
| title_sort | occurrence of non o157 shiga toxin producing escherichia coli in healthy cattle and goats and distribution of virulence genes among isolates |
| url | http://psasir.upm.edu.my/id/eprint/29850/1/29850.pdf |
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