A pH-sensitive, biobased calcium carbonate aragonite nanocrystal as a novel anticancer delivery system
The synthesised biobased calcium carbonate nanocrystals had demonstrated to be an effective carrier for delivery of anticancer drug doxorubicin (DOX). The use of these nanocrystals displayed high levels of selectivity and specificity in achieving effective cancer cell death without nonspecific toxic...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29878/1/A%20pH.pdf |
| _version_ | 1825947597576077312 |
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| author | Kamba, Abdullahi Shafiu Ismail, Maznah Tengku Ibrahim, Tengku Azmi Abu Bakar @ Zakaria, Md Zuki |
| author_facet | Kamba, Abdullahi Shafiu Ismail, Maznah Tengku Ibrahim, Tengku Azmi Abu Bakar @ Zakaria, Md Zuki |
| author_sort | Kamba, Abdullahi Shafiu |
| collection | UPM |
| description | The synthesised biobased calcium carbonate nanocrystals had demonstrated to be an effective carrier for delivery of anticancer drug doxorubicin (DOX). The use of these nanocrystals displayed high levels of selectivity and specificity in achieving effective cancer cell death without nonspecific toxicity. These results confirmed that DOX was intercalated into calcium carbonate nanocrystals at high loading and encapsulation efficiency (4.8 and 96%, resp.). The CaCO₃/DOX nanocrystals are relatively stable at neutral pH (7.4), resulting in slow release, but the nanocrystals progressively dissociated in acidic pH (4.8) regimes, triggering faster release of DOX. The CaCO₃/DOX nanocrystals exhibited high uptake by MDA MB231 breast cancer cells and a promising potential delivery of DOX to target cells. In vitro chemosensitivity using MTT, modified neutral red/trypan blue assay, and LDH on MDA MB231 breast cancer cells revealed that CaCO₃/DOX nanocrystals are more sensitive and gave a greater reduction in cell growth than free DOX. Our findings suggest that CaCO₃ nanocrystals hold tremendous promise in the areas of controlled drug delivery and targeted cancer therapy. |
| first_indexed | 2024-03-06T08:15:49Z |
| format | Article |
| id | upm.eprints-29878 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:15:49Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-298782016-11-18T07:50:03Z http://psasir.upm.edu.my/id/eprint/29878/ A pH-sensitive, biobased calcium carbonate aragonite nanocrystal as a novel anticancer delivery system Kamba, Abdullahi Shafiu Ismail, Maznah Tengku Ibrahim, Tengku Azmi Abu Bakar @ Zakaria, Md Zuki The synthesised biobased calcium carbonate nanocrystals had demonstrated to be an effective carrier for delivery of anticancer drug doxorubicin (DOX). The use of these nanocrystals displayed high levels of selectivity and specificity in achieving effective cancer cell death without nonspecific toxicity. These results confirmed that DOX was intercalated into calcium carbonate nanocrystals at high loading and encapsulation efficiency (4.8 and 96%, resp.). The CaCO₃/DOX nanocrystals are relatively stable at neutral pH (7.4), resulting in slow release, but the nanocrystals progressively dissociated in acidic pH (4.8) regimes, triggering faster release of DOX. The CaCO₃/DOX nanocrystals exhibited high uptake by MDA MB231 breast cancer cells and a promising potential delivery of DOX to target cells. In vitro chemosensitivity using MTT, modified neutral red/trypan blue assay, and LDH on MDA MB231 breast cancer cells revealed that CaCO₃/DOX nanocrystals are more sensitive and gave a greater reduction in cell growth than free DOX. Our findings suggest that CaCO₃ nanocrystals hold tremendous promise in the areas of controlled drug delivery and targeted cancer therapy. Hindawi Publishing Corporation 2013-10-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29878/1/A%20pH.pdf Kamba, Abdullahi Shafiu and Ismail, Maznah and Tengku Ibrahim, Tengku Azmi and Abu Bakar @ Zakaria, Md Zuki (2013) A pH-sensitive, biobased calcium carbonate aragonite nanocrystal as a novel anticancer delivery system. BioMed Research International, 2013. art. no. 587451. pp. 1-10. ISSN 2314-6133; ESSN: 2314-6141 https://www.hindawi.com/journals/bmri/2013/587451/abs/ 10.1155/2013/587451 |
| spellingShingle | Kamba, Abdullahi Shafiu Ismail, Maznah Tengku Ibrahim, Tengku Azmi Abu Bakar @ Zakaria, Md Zuki A pH-sensitive, biobased calcium carbonate aragonite nanocrystal as a novel anticancer delivery system |
| title | A pH-sensitive, biobased calcium carbonate aragonite nanocrystal as a novel anticancer delivery system |
| title_full | A pH-sensitive, biobased calcium carbonate aragonite nanocrystal as a novel anticancer delivery system |
| title_fullStr | A pH-sensitive, biobased calcium carbonate aragonite nanocrystal as a novel anticancer delivery system |
| title_full_unstemmed | A pH-sensitive, biobased calcium carbonate aragonite nanocrystal as a novel anticancer delivery system |
| title_short | A pH-sensitive, biobased calcium carbonate aragonite nanocrystal as a novel anticancer delivery system |
| title_sort | ph sensitive biobased calcium carbonate aragonite nanocrystal as a novel anticancer delivery system |
| url | http://psasir.upm.edu.my/id/eprint/29878/1/A%20pH.pdf |
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