Effect of semen collection methods on the quality of pre- and post-thawed Bali cattle (Bos javanicus) spermatozoa.
This study was conducted to evaluate the response of Bali bulls (Bos javanicus) to different semen collection methods and their effects on fresh and post-thawed semen quality. The collection methods employed were electro-ejaculation (EE), transrectal massage (RM) and RM followed by EE (RM + EE). A t...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Wiley-Blackwell
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29905/1/Effect%20of%20semen%20collection%20methods%20on%20the%20quality%20of%20pre.pdf |
| _version_ | 1796971634163187712 |
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| author | Sarsaifi, Kazhal Yusoff, Rosnina Omar, Mohamed Ariff Haron, Abd Wahid Hani, Homayoun Nurhusien, Yimer Vejayan, Jaya Naing, Soe Win Othman Abas, Mazni |
| author_facet | Sarsaifi, Kazhal Yusoff, Rosnina Omar, Mohamed Ariff Haron, Abd Wahid Hani, Homayoun Nurhusien, Yimer Vejayan, Jaya Naing, Soe Win Othman Abas, Mazni |
| author_sort | Sarsaifi, Kazhal |
| collection | UPM |
| description | This study was conducted to evaluate the response of Bali bulls (Bos javanicus) to different semen collection methods and their effects on fresh and post-thawed semen quality. The collection methods employed were electro-ejaculation (EE), transrectal massage (RM) and RM followed by EE (RM + EE). A total of 25 untrained Bali bulls (age between 2 and 4 years old) were subjected to the different semen collection methods. Fresh semen samples from all the 25 bulls were evaluated for volume, pH, general motility, live/dead ratio and abnormality using the conventional method. For fresh and frozen samples collected by EE and RM from 10 bulls, computer-assisted semen analysis system was used for precise quantitative measurement of motility, velocity and forward progression. Accucell photometer was used to measure sperm concentration in all samples, regardless fresh and frozen. Semen samples were obtained 100% of the attempts using EE, 84% using RM and 96% using RM + EE. There were no differences among the collection methods for fresh semen quality characteristics, including motility, morphology and viability, but pH and volume were higher for EE than RM and RM + EE. Higher sperm concentration was observed in semen collected by RM than the other two methods. Different age groups (2–3 and >3–4 years old) of the bulls did not show significant differences in volume, pH, sperm concentration, percentages in motility, live/dead ratio and normal sperm morphology. The quality of semen for general and progressive motility, VAP, VSL and VCL and acrosomal integrity after thawing was higher for RM than EE. In conclusion, Bali bulls appeared to respond best to EE and the combination of RM + EE than RM, as a method of semen collection, with a shorter time of stimulation required. Differences in age of the Bali bulls did not affect the semen quality. |
| first_indexed | 2024-03-06T08:15:54Z |
| format | Article |
| id | upm.eprints-29905 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:15:54Z |
| publishDate | 2013 |
| publisher | Wiley-Blackwell |
| record_format | dspace |
| spelling | upm.eprints-299052016-02-03T03:33:39Z http://psasir.upm.edu.my/id/eprint/29905/ Effect of semen collection methods on the quality of pre- and post-thawed Bali cattle (Bos javanicus) spermatozoa. Sarsaifi, Kazhal Yusoff, Rosnina Omar, Mohamed Ariff Haron, Abd Wahid Hani, Homayoun Nurhusien, Yimer Vejayan, Jaya Naing, Soe Win Othman Abas, Mazni This study was conducted to evaluate the response of Bali bulls (Bos javanicus) to different semen collection methods and their effects on fresh and post-thawed semen quality. The collection methods employed were electro-ejaculation (EE), transrectal massage (RM) and RM followed by EE (RM + EE). A total of 25 untrained Bali bulls (age between 2 and 4 years old) were subjected to the different semen collection methods. Fresh semen samples from all the 25 bulls were evaluated for volume, pH, general motility, live/dead ratio and abnormality using the conventional method. For fresh and frozen samples collected by EE and RM from 10 bulls, computer-assisted semen analysis system was used for precise quantitative measurement of motility, velocity and forward progression. Accucell photometer was used to measure sperm concentration in all samples, regardless fresh and frozen. Semen samples were obtained 100% of the attempts using EE, 84% using RM and 96% using RM + EE. There were no differences among the collection methods for fresh semen quality characteristics, including motility, morphology and viability, but pH and volume were higher for EE than RM and RM + EE. Higher sperm concentration was observed in semen collected by RM than the other two methods. Different age groups (2–3 and >3–4 years old) of the bulls did not show significant differences in volume, pH, sperm concentration, percentages in motility, live/dead ratio and normal sperm morphology. The quality of semen for general and progressive motility, VAP, VSL and VCL and acrosomal integrity after thawing was higher for RM than EE. In conclusion, Bali bulls appeared to respond best to EE and the combination of RM + EE than RM, as a method of semen collection, with a shorter time of stimulation required. Differences in age of the Bali bulls did not affect the semen quality. Wiley-Blackwell 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29905/1/Effect%20of%20semen%20collection%20methods%20on%20the%20quality%20of%20pre.pdf Sarsaifi, Kazhal and Yusoff, Rosnina and Omar, Mohamed Ariff and Haron, Abd Wahid and Hani, Homayoun and Nurhusien, Yimer and Vejayan, Jaya and Naing, Soe Win and Othman Abas, Mazni (2013) Effect of semen collection methods on the quality of pre- and post-thawed Bali cattle (Bos javanicus) spermatozoa. Reproduction in Domestic Animals, 48 (6). pp. 1006-1012. ISSN 0936-6768; ESSN: 1439-0531 10.1111/rda.12206 English |
| spellingShingle | Sarsaifi, Kazhal Yusoff, Rosnina Omar, Mohamed Ariff Haron, Abd Wahid Hani, Homayoun Nurhusien, Yimer Vejayan, Jaya Naing, Soe Win Othman Abas, Mazni Effect of semen collection methods on the quality of pre- and post-thawed Bali cattle (Bos javanicus) spermatozoa. |
| title | Effect of semen collection methods on the quality of pre- and post-thawed Bali cattle (Bos javanicus) spermatozoa. |
| title_full | Effect of semen collection methods on the quality of pre- and post-thawed Bali cattle (Bos javanicus) spermatozoa. |
| title_fullStr | Effect of semen collection methods on the quality of pre- and post-thawed Bali cattle (Bos javanicus) spermatozoa. |
| title_full_unstemmed | Effect of semen collection methods on the quality of pre- and post-thawed Bali cattle (Bos javanicus) spermatozoa. |
| title_short | Effect of semen collection methods on the quality of pre- and post-thawed Bali cattle (Bos javanicus) spermatozoa. |
| title_sort | effect of semen collection methods on the quality of pre and post thawed bali cattle bos javanicus spermatozoa |
| url | http://psasir.upm.edu.my/id/eprint/29905/1/Effect%20of%20semen%20collection%20methods%20on%20the%20quality%20of%20pre.pdf |
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