Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia
Apart from occasional reports of clinical disease affecting horses, there is no information about Trypanosoma evansi in horses in Peninsula Malaysia. Thus, a cross-sectional study was conducted in eight states in Peninsula Malaysia to determine the active presence of T. evansi in horses. A total of...
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| Format: | Article |
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Malaysian Society of Parasitology and Tropical Medicine
2013
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| _version_ | 1825947606862266368 |
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| author | Elshafie, E. I. Abdullah Sani, Rehana Hassan, Latiffah Kumar Sharma, Reuben Sunil Fateh Mohamed, Bashir Ahmad Abubakar, I. A. |
| author_facet | Elshafie, E. I. Abdullah Sani, Rehana Hassan, Latiffah Kumar Sharma, Reuben Sunil Fateh Mohamed, Bashir Ahmad Abubakar, I. A. |
| author_sort | Elshafie, E. I. |
| collection | UPM |
| description | Apart from occasional reports of clinical disease affecting horses, there is no information about Trypanosoma evansi in horses in Peninsula Malaysia. Thus, a cross-sectional
study was conducted in eight states in Peninsula Malaysia to determine the active presence of T. evansi in horses. A total of 527 blood samples were obtained and examined by haematocrit centrifugation technique (HCT), Giemsa-stained thin blood smear (GSS), morphometric measurements, polymerase chain reaction (PCR) and cloning of PCR products. The results showed an overall parasitological prevalence of 0.57% (3/527, CI: 1.6-0.19%) with both HCT and GSS. Morphometric study revealed the mean total length of the trypanosomes including the free flagellum was 27.94 ± 2.63 µm. PCR successfully amplified a trypanosome specific
257 bp in 1.14% of samples (6/527, CI: 2.4-0.52%) and was confirmed by nucleotide sequences. The mean packed cell volume (PCV) for the positive cases detected by HCT was lower (23% ± 7.00) compared to the positive cases detected by PCR alone in the state of Terengganu (35% ± 4.73). In conclusion, this study showed T. evansi infection occurred in low frequency in horses in Peninsula Malaysia, and anaemia coincided with parasitaemic animals. PCR is
considered as a sensitive diagnostic tool when parasitaemia is undetectable. The slight lengthier mean of parasite and anaemia may indicate a virulent strain of T. evansi circulating throughout the country. Thus, it’s highly recommended to shed light on host-parasite relationship
for better epidemiological understanding. |
| first_indexed | 2024-03-06T08:15:58Z |
| format | Article |
| id | upm.eprints-29926 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:15:58Z |
| publishDate | 2013 |
| publisher | Malaysian Society of Parasitology and Tropical Medicine |
| record_format | dspace |
| spelling | upm.eprints-299262016-01-20T05:28:33Z http://psasir.upm.edu.my/id/eprint/29926/ Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia Elshafie, E. I. Abdullah Sani, Rehana Hassan, Latiffah Kumar Sharma, Reuben Sunil Fateh Mohamed, Bashir Ahmad Abubakar, I. A. Apart from occasional reports of clinical disease affecting horses, there is no information about Trypanosoma evansi in horses in Peninsula Malaysia. Thus, a cross-sectional study was conducted in eight states in Peninsula Malaysia to determine the active presence of T. evansi in horses. A total of 527 blood samples were obtained and examined by haematocrit centrifugation technique (HCT), Giemsa-stained thin blood smear (GSS), morphometric measurements, polymerase chain reaction (PCR) and cloning of PCR products. The results showed an overall parasitological prevalence of 0.57% (3/527, CI: 1.6-0.19%) with both HCT and GSS. Morphometric study revealed the mean total length of the trypanosomes including the free flagellum was 27.94 ± 2.63 µm. PCR successfully amplified a trypanosome specific 257 bp in 1.14% of samples (6/527, CI: 2.4-0.52%) and was confirmed by nucleotide sequences. The mean packed cell volume (PCV) for the positive cases detected by HCT was lower (23% ± 7.00) compared to the positive cases detected by PCR alone in the state of Terengganu (35% ± 4.73). In conclusion, this study showed T. evansi infection occurred in low frequency in horses in Peninsula Malaysia, and anaemia coincided with parasitaemic animals. PCR is considered as a sensitive diagnostic tool when parasitaemia is undetectable. The slight lengthier mean of parasite and anaemia may indicate a virulent strain of T. evansi circulating throughout the country. Thus, it’s highly recommended to shed light on host-parasite relationship for better epidemiological understanding. Malaysian Society of Parasitology and Tropical Medicine 2013 Article PeerReviewed Elshafie, E. I. and Abdullah Sani, Rehana and Hassan, Latiffah and Kumar Sharma, Reuben Sunil and Fateh Mohamed, Bashir Ahmad and Abubakar, I. A. (2013) Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia. Tropical Biomedicine, 30 (3). pp. 444-450. ISSN 0127-5720 http://www.msptm.org/journal27.html |
| spellingShingle | Elshafie, E. I. Abdullah Sani, Rehana Hassan, Latiffah Kumar Sharma, Reuben Sunil Fateh Mohamed, Bashir Ahmad Abubakar, I. A. Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia |
| title | Active infection and morphometric study of Trypanosoma
evansi among horses in Peninsula Malaysia |
| title_full | Active infection and morphometric study of Trypanosoma
evansi among horses in Peninsula Malaysia |
| title_fullStr | Active infection and morphometric study of Trypanosoma
evansi among horses in Peninsula Malaysia |
| title_full_unstemmed | Active infection and morphometric study of Trypanosoma
evansi among horses in Peninsula Malaysia |
| title_short | Active infection and morphometric study of Trypanosoma
evansi among horses in Peninsula Malaysia |
| title_sort | active infection and morphometric study of trypanosoma evansi among horses in peninsula malaysia |
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