Comparative genome structure, secondary metabolite, and effector coding capacity across Cochliobolus pathogens.
The genomes of five Cochliobolus heterostrophus strains, two Cochliobolus sativus strains, three additional Cochliobolus species (Cochliobolus victoriae, Cochliobolus carbonum, Cochliobolus miyabeanus), and closely related Setosphaeria turcica were sequenced at the Joint Genome Institute (JGI). The...
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| Format: | Article |
| Language: | English English |
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Public Library of Science
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30002/1/Comparative%20genome%20structure.pdf |
| _version_ | 1825947621334712320 |
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| author | Condon, Bradford J. Leng, Yueqiang Wu, Dongliang Bushley, Kathryn E. Ohm, Robin A. Martin, Joel Schackwitz, Wendy Grimwood, Jane Mohd Zainudin, Nur Ain Izzati Xue, Chunsheng Wang, Rui Manning, Viola A. Dhillon, Braham Tu, Zheng Jin Steffenson, Brian J. Salamov, Asaf Sun, Hui Lowry, Steve LaButti, Kurt Han, James Copeland, Alex Lindquist, Erika Barry, Kerrie Schmutz, Jeremy Scott E., Scott E. Ciuffetti, Lynda M. Grigoriev, Igor V. Zhong, Shaobin Turgeon, B. Gillian |
| author_facet | Condon, Bradford J. Leng, Yueqiang Wu, Dongliang Bushley, Kathryn E. Ohm, Robin A. Martin, Joel Schackwitz, Wendy Grimwood, Jane Mohd Zainudin, Nur Ain Izzati Xue, Chunsheng Wang, Rui Manning, Viola A. Dhillon, Braham Tu, Zheng Jin Steffenson, Brian J. Salamov, Asaf Sun, Hui Lowry, Steve LaButti, Kurt Han, James Copeland, Alex Lindquist, Erika Barry, Kerrie Schmutz, Jeremy Scott E., Scott E. Ciuffetti, Lynda M. Grigoriev, Igor V. Zhong, Shaobin Turgeon, B. Gillian |
| author_sort | Condon, Bradford J. |
| collection | UPM |
| description | The genomes of five Cochliobolus heterostrophus strains, two Cochliobolus sativus strains, three additional Cochliobolus species (Cochliobolus victoriae, Cochliobolus carbonum, Cochliobolus miyabeanus), and closely related Setosphaeria turcica were sequenced at the Joint Genome Institute (JGI). The datasets were used to identify SNPs between strains and species, unique genomic regions, core secondary metabolism genes, and small secreted protein (SSP) candidate effector encoding genes with a view towards pinpointing structural elements and gene content associated with specificity of these closely related fungi to different cereal hosts. Whole-genome alignment shows that three to five percent of each genome differs between strains of the same species, while a quarter of each genome differs between species. On average, SNP counts among field isolates of the same C. heterostrophus species are more than 25× higher than those between inbred lines and 50× lower than SNPs between Cochliobolus species. The suites of nonribosomal peptide synthetase (NRPS), polyketide synthase (PKS), and SSP-encoding genes are astoundingly diverse among species but remarkably conserved among isolates of the same species, whether inbred or field strains, except for defining examples that map to unique genomic regions. Functional analysis of several strain-unique PKSs and NRPSs reveal a strong correlation with a role in virulence. |
| first_indexed | 2024-03-06T08:16:11Z |
| format | Article |
| id | upm.eprints-30002 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:16:11Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | upm.eprints-300022015-09-09T01:17:40Z http://psasir.upm.edu.my/id/eprint/30002/ Comparative genome structure, secondary metabolite, and effector coding capacity across Cochliobolus pathogens. Condon, Bradford J. Leng, Yueqiang Wu, Dongliang Bushley, Kathryn E. Ohm, Robin A. Martin, Joel Schackwitz, Wendy Grimwood, Jane Mohd Zainudin, Nur Ain Izzati Xue, Chunsheng Wang, Rui Manning, Viola A. Dhillon, Braham Tu, Zheng Jin Steffenson, Brian J. Salamov, Asaf Sun, Hui Lowry, Steve LaButti, Kurt Han, James Copeland, Alex Lindquist, Erika Barry, Kerrie Schmutz, Jeremy Scott E., Scott E. Ciuffetti, Lynda M. Grigoriev, Igor V. Zhong, Shaobin Turgeon, B. Gillian The genomes of five Cochliobolus heterostrophus strains, two Cochliobolus sativus strains, three additional Cochliobolus species (Cochliobolus victoriae, Cochliobolus carbonum, Cochliobolus miyabeanus), and closely related Setosphaeria turcica were sequenced at the Joint Genome Institute (JGI). The datasets were used to identify SNPs between strains and species, unique genomic regions, core secondary metabolism genes, and small secreted protein (SSP) candidate effector encoding genes with a view towards pinpointing structural elements and gene content associated with specificity of these closely related fungi to different cereal hosts. Whole-genome alignment shows that three to five percent of each genome differs between strains of the same species, while a quarter of each genome differs between species. On average, SNP counts among field isolates of the same C. heterostrophus species are more than 25× higher than those between inbred lines and 50× lower than SNPs between Cochliobolus species. The suites of nonribosomal peptide synthetase (NRPS), polyketide synthase (PKS), and SSP-encoding genes are astoundingly diverse among species but remarkably conserved among isolates of the same species, whether inbred or field strains, except for defining examples that map to unique genomic regions. Functional analysis of several strain-unique PKSs and NRPSs reveal a strong correlation with a role in virulence. Public Library of Science 2013-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30002/1/Comparative%20genome%20structure.pdf Condon, Bradford J. and Leng, Yueqiang and Wu, Dongliang and Bushley, Kathryn E. and Ohm, Robin A. and Martin, Joel and Schackwitz, Wendy and Grimwood, Jane and Mohd Zainudin, Nur Ain Izzati and Xue, Chunsheng and Wang, Rui and Manning, Viola A. and Dhillon, Braham and Tu, Zheng Jin and Steffenson, Brian J. and Salamov, Asaf and Sun, Hui and Lowry, Steve and LaButti, Kurt and Han, James and Copeland, Alex and Lindquist, Erika and Barry, Kerrie and Schmutz, Jeremy and Scott E., Scott E. and Ciuffetti, Lynda M. and Grigoriev, Igor V. and Zhong, Shaobin and Turgeon, B. Gillian (2013) Comparative genome structure, secondary metabolite, and effector coding capacity across Cochliobolus pathogens. PLOS Genetics, 9 (1). pp. 1-29. ISSN 1553-7390: ESSN: 1553-7404 10.1371/journal.pgen.1003233 English |
| spellingShingle | Condon, Bradford J. Leng, Yueqiang Wu, Dongliang Bushley, Kathryn E. Ohm, Robin A. Martin, Joel Schackwitz, Wendy Grimwood, Jane Mohd Zainudin, Nur Ain Izzati Xue, Chunsheng Wang, Rui Manning, Viola A. Dhillon, Braham Tu, Zheng Jin Steffenson, Brian J. Salamov, Asaf Sun, Hui Lowry, Steve LaButti, Kurt Han, James Copeland, Alex Lindquist, Erika Barry, Kerrie Schmutz, Jeremy Scott E., Scott E. Ciuffetti, Lynda M. Grigoriev, Igor V. Zhong, Shaobin Turgeon, B. Gillian Comparative genome structure, secondary metabolite, and effector coding capacity across Cochliobolus pathogens. |
| title | Comparative genome structure, secondary metabolite, and effector coding capacity across Cochliobolus pathogens.
|
| title_full | Comparative genome structure, secondary metabolite, and effector coding capacity across Cochliobolus pathogens.
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| title_fullStr | Comparative genome structure, secondary metabolite, and effector coding capacity across Cochliobolus pathogens.
|
| title_full_unstemmed | Comparative genome structure, secondary metabolite, and effector coding capacity across Cochliobolus pathogens.
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| title_short | Comparative genome structure, secondary metabolite, and effector coding capacity across Cochliobolus pathogens.
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| title_sort | comparative genome structure secondary metabolite and effector coding capacity across cochliobolus pathogens |
| url | http://psasir.upm.edu.my/id/eprint/30002/1/Comparative%20genome%20structure.pdf |
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