DNA-based biosensor for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium complex [Ru(phen)2,(qtpy)]2+ based on a PEDOT-PSS/Ag nanoparticles modified electrode
An electrochemical DNA biosensor has been developed for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium [Ru(phen)2,(qtpy)]2+ complex as hybridization indicator. The sensor incorporated the use of a gold electrode (AuE), modified with a conducting nanocomp...
| Príomhchruthaitheoirí: | , , , , , |
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| Formáid: | Alt |
| Teanga: | English English |
| Foilsithe / Cruthaithe: |
Electrochemical Science Group
2013
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| Rochtain ar líne: | http://psasir.upm.edu.my/id/eprint/30183/1/DNA.pdf |
| _version_ | 1825947656688500736 |
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| author | Dutse, Sabo Wada Yusof, Nor Azah Ahmad , Haslina Hussein, Mohd Zobir Zainal, Zulkarnain Hushiarian, Roozbeh |
| author_facet | Dutse, Sabo Wada Yusof, Nor Azah Ahmad , Haslina Hussein, Mohd Zobir Zainal, Zulkarnain Hushiarian, Roozbeh |
| author_sort | Dutse, Sabo Wada |
| collection | UPM |
| description | An electrochemical DNA biosensor has been developed for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium [Ru(phen)2,(qtpy)]2+ complex as hybridization indicator. The sensor incorporated the use of a gold electrode (AuE), modified with a conducting nanocomposite of poly(3,4-ethylene-dioxythiophen) -poly(styrenesulfonate) (PEDOT-PSS) and silver nanoparticles (AgNPs). A specific sequence of a ganoderma boninense DNA probe has been immobilized on the modified electrode and the hybridization event was monitored via intercalation of the ruthenium complex to the hybridized DNA. Effect of hybridization temperature and time was evaluated and found to be optimal at 45 °C in 25 minutes for the hybridization. Detection of target DNA ranged from 1.0 x 10-15 M to 1.0 × 10-9 M was performed, and a correlation relationship of 0.9756 and detection limit of 5 × 10-16 M were obtained. The newly synthesized ruthenium complex was able to be used is a novel redox marker and can be adopted for routine detection of DNA. |
| first_indexed | 2024-03-06T08:16:44Z |
| format | Article |
| id | upm.eprints-30183 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:16:44Z |
| publishDate | 2013 |
| publisher | Electrochemical Science Group |
| record_format | dspace |
| spelling | upm.eprints-301832015-10-07T06:54:35Z http://psasir.upm.edu.my/id/eprint/30183/ DNA-based biosensor for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium complex [Ru(phen)2,(qtpy)]2+ based on a PEDOT-PSS/Ag nanoparticles modified electrode Dutse, Sabo Wada Yusof, Nor Azah Ahmad , Haslina Hussein, Mohd Zobir Zainal, Zulkarnain Hushiarian, Roozbeh An electrochemical DNA biosensor has been developed for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium [Ru(phen)2,(qtpy)]2+ complex as hybridization indicator. The sensor incorporated the use of a gold electrode (AuE), modified with a conducting nanocomposite of poly(3,4-ethylene-dioxythiophen) -poly(styrenesulfonate) (PEDOT-PSS) and silver nanoparticles (AgNPs). A specific sequence of a ganoderma boninense DNA probe has been immobilized on the modified electrode and the hybridization event was monitored via intercalation of the ruthenium complex to the hybridized DNA. Effect of hybridization temperature and time was evaluated and found to be optimal at 45 °C in 25 minutes for the hybridization. Detection of target DNA ranged from 1.0 x 10-15 M to 1.0 × 10-9 M was performed, and a correlation relationship of 0.9756 and detection limit of 5 × 10-16 M were obtained. The newly synthesized ruthenium complex was able to be used is a novel redox marker and can be adopted for routine detection of DNA. Electrochemical Science Group 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30183/1/DNA.pdf Dutse, Sabo Wada and Yusof, Nor Azah and Ahmad , Haslina and Hussein, Mohd Zobir and Zainal, Zulkarnain and Hushiarian, Roozbeh (2013) DNA-based biosensor for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium complex [Ru(phen)2,(qtpy)]2+ based on a PEDOT-PSS/Ag nanoparticles modified electrode. International Journal of Electrochemical Science, 8 (9). pp. 11048-11057. ISSN 1452-3981 http://www.electrochemsci.org/list13.htm#issue9 English |
| spellingShingle | Dutse, Sabo Wada Yusof, Nor Azah Ahmad , Haslina Hussein, Mohd Zobir Zainal, Zulkarnain Hushiarian, Roozbeh DNA-based biosensor for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium complex [Ru(phen)2,(qtpy)]2+ based on a PEDOT-PSS/Ag nanoparticles modified electrode |
| title | DNA-based biosensor for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium complex [Ru(phen)2,(qtpy)]2+ based on a PEDOT-PSS/Ag nanoparticles modified electrode |
| title_full | DNA-based biosensor for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium complex [Ru(phen)2,(qtpy)]2+ based on a PEDOT-PSS/Ag nanoparticles modified electrode |
| title_fullStr | DNA-based biosensor for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium complex [Ru(phen)2,(qtpy)]2+ based on a PEDOT-PSS/Ag nanoparticles modified electrode |
| title_full_unstemmed | DNA-based biosensor for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium complex [Ru(phen)2,(qtpy)]2+ based on a PEDOT-PSS/Ag nanoparticles modified electrode |
| title_short | DNA-based biosensor for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium complex [Ru(phen)2,(qtpy)]2+ based on a PEDOT-PSS/Ag nanoparticles modified electrode |
| title_sort | dna based biosensor for detection of ganoderma boninense an oil palm pathogen utilizing newly synthesized ruthenium complex ru phen 2 qtpy 2 based on a pedot pss ag nanoparticles modified electrode |
| url | http://psasir.upm.edu.my/id/eprint/30183/1/DNA.pdf |
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