Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies
Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multil...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Springer
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30206/1/Identification%20of%20hybrids%20of%20painted%20and%20milky%20storks%20using%20FTA%20card.pdf |
| _version_ | 1796971694326284288 |
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| author | Yee, Elsie Yoke Sim Zainuddin, Zainal Zahari Ismail, Ahmad Yap, Chee Kong Tan, Soon Guan |
| author_facet | Yee, Elsie Yoke Sim Zainuddin, Zainal Zahari Ismail, Ahmad Yap, Chee Kong Tan, Soon Guan |
| author_sort | Yee, Elsie Yoke Sim |
| collection | UPM |
| description | Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patterns that provided two sets of genetic data, one for each of the two stork species and another for the hybrids. It also revealed that large DNA fragments (3,000 bp) could be amplified from blood collected on FTA cards. When the results of each individual bird’s DNA fingerprint were compared with plumage characters, the hybrids were found to express a range of intermediate phenotypic traits of the pure breeds with no dominant plumage characteristic from either parental species. |
| first_indexed | 2024-03-06T08:16:48Z |
| format | Article |
| id | upm.eprints-30206 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:16:48Z |
| publishDate | 2013 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-302062015-10-23T03:34:01Z http://psasir.upm.edu.my/id/eprint/30206/ Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies Yee, Elsie Yoke Sim Zainuddin, Zainal Zahari Ismail, Ahmad Yap, Chee Kong Tan, Soon Guan Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patterns that provided two sets of genetic data, one for each of the two stork species and another for the hybrids. It also revealed that large DNA fragments (3,000 bp) could be amplified from blood collected on FTA cards. When the results of each individual bird’s DNA fingerprint were compared with plumage characters, the hybrids were found to express a range of intermediate phenotypic traits of the pure breeds with no dominant plumage characteristic from either parental species. Springer 2013-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30206/1/Identification%20of%20hybrids%20of%20painted%20and%20milky%20storks%20using%20FTA%20card.pdf Yee, Elsie Yoke Sim and Zainuddin, Zainal Zahari and Ismail, Ahmad and Yap, Chee Kong and Tan, Soon Guan (2013) Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies. Biochemical Genetics, 51 (9-10). pp. 789-799. ISSN 0006-2928; ESSN: 1573-4927 10.1007/s10528-013-9607-8 English |
| spellingShingle | Yee, Elsie Yoke Sim Zainuddin, Zainal Zahari Ismail, Ahmad Yap, Chee Kong Tan, Soon Guan Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies |
| title | Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies |
| title_full | Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies |
| title_fullStr | Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies |
| title_full_unstemmed | Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies |
| title_short | Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies |
| title_sort | identification of hybrids of painted and milky storks using fta card collected blood molecular markers and morphologies |
| url | http://psasir.upm.edu.my/id/eprint/30206/1/Identification%20of%20hybrids%20of%20painted%20and%20milky%20storks%20using%20FTA%20card.pdf |
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