Quantitative HPLC analysis of benzene derivatives of Melicope ptelefolia leaves
A high performance liquid chromatography procedure for the quantitative determination of three marker benzene derivatives, 2,4,6-trihydroxy-3-prenyl acetophenone (tHPA) (1), 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA) (2), and p-O-geranyl coumaric acid (GCA) (3), in the Melicope ptelefolia ethano...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Taylor & Francis
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30260/1/Quantitative%20HPLC%20analysis%20of%20benzene%20derivatives%20of%20Melicope%20ptelefolia%20leaves.pdf |
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| author | Zareen, Seema Ismail, Intan Safinar Shaari, Khozirah Yusof, Khairul Nazri Akhtar, Muhammad Nadeem |
| author_facet | Zareen, Seema Ismail, Intan Safinar Shaari, Khozirah Yusof, Khairul Nazri Akhtar, Muhammad Nadeem |
| author_sort | Zareen, Seema |
| collection | UPM |
| description | A high performance liquid chromatography procedure for the quantitative determination of three marker benzene derivatives, 2,4,6-trihydroxy-3-prenyl acetophenone (tHPA) (1), 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA) (2), and p-O-geranyl coumaric acid (GCA) (3), in the Melicope ptelefolia ethanolic leaf extracts, a medicinal herb obtained from a few locations of the Peninsula Malaysia, was described. The quantitative analysis was performed using high performance liquid chromatography-photodiode array detection on Xterra octadecylsiyl silica (ODS; 3.0 × 150 mm, 3.5 μm) column kept at 32°C, using gradient elution with acetonitrile and water containing 0.1% formic acid at a flow-rate of 1 ml/min with UV detection wavelength at 280 nm. All calibration curves showed good linearity (R2 of 0.999 to 1.0000) within the concentrations range of 2.5 × 10-3 to 0.1 mg/mL. The method was shown to be simple, sensitive, and reliable for qualitative and quantitative analysis of the marker compounds in M. ptelefolia leaf preparations. |
| first_indexed | 2024-03-06T08:16:58Z |
| format | Article |
| id | upm.eprints-30260 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:16:58Z |
| publishDate | 2013 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-302602015-11-17T03:43:03Z http://psasir.upm.edu.my/id/eprint/30260/ Quantitative HPLC analysis of benzene derivatives of Melicope ptelefolia leaves Zareen, Seema Ismail, Intan Safinar Shaari, Khozirah Yusof, Khairul Nazri Akhtar, Muhammad Nadeem A high performance liquid chromatography procedure for the quantitative determination of three marker benzene derivatives, 2,4,6-trihydroxy-3-prenyl acetophenone (tHPA) (1), 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA) (2), and p-O-geranyl coumaric acid (GCA) (3), in the Melicope ptelefolia ethanolic leaf extracts, a medicinal herb obtained from a few locations of the Peninsula Malaysia, was described. The quantitative analysis was performed using high performance liquid chromatography-photodiode array detection on Xterra octadecylsiyl silica (ODS; 3.0 × 150 mm, 3.5 μm) column kept at 32°C, using gradient elution with acetonitrile and water containing 0.1% formic acid at a flow-rate of 1 ml/min with UV detection wavelength at 280 nm. All calibration curves showed good linearity (R2 of 0.999 to 1.0000) within the concentrations range of 2.5 × 10-3 to 0.1 mg/mL. The method was shown to be simple, sensitive, and reliable for qualitative and quantitative analysis of the marker compounds in M. ptelefolia leaf preparations. Taylor & Francis 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30260/1/Quantitative%20HPLC%20analysis%20of%20benzene%20derivatives%20of%20Melicope%20ptelefolia%20leaves.pdf Zareen, Seema and Ismail, Intan Safinar and Shaari, Khozirah and Yusof, Khairul Nazri and Akhtar, Muhammad Nadeem (2013) Quantitative HPLC analysis of benzene derivatives of Melicope ptelefolia leaves. International Journal of Food Properties, 16 (8). pp. 1830-1838. ISSN 1094-2912; ESSN: 1532-2386 10.1080/10942912.2011.609952 English |
| spellingShingle | Zareen, Seema Ismail, Intan Safinar Shaari, Khozirah Yusof, Khairul Nazri Akhtar, Muhammad Nadeem Quantitative HPLC analysis of benzene derivatives of Melicope ptelefolia leaves |
| title | Quantitative HPLC analysis of benzene derivatives of Melicope ptelefolia leaves |
| title_full | Quantitative HPLC analysis of benzene derivatives of Melicope ptelefolia leaves |
| title_fullStr | Quantitative HPLC analysis of benzene derivatives of Melicope ptelefolia leaves |
| title_full_unstemmed | Quantitative HPLC analysis of benzene derivatives of Melicope ptelefolia leaves |
| title_short | Quantitative HPLC analysis of benzene derivatives of Melicope ptelefolia leaves |
| title_sort | quantitative hplc analysis of benzene derivatives of melicope ptelefolia leaves |
| url | http://psasir.upm.edu.my/id/eprint/30260/1/Quantitative%20HPLC%20analysis%20of%20benzene%20derivatives%20of%20Melicope%20ptelefolia%20leaves.pdf |
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