Extraction methods effects on composition and toxicity of Eucalyptus essential oil

The current investigation was designed to determine the influence of three extraction methods on the composition and insecticidal activity of essential oils extracted from Eucalyptus globulus and E. camaldulensis. The evaluated extraction methods were steam-distillation, steam water-distillation a...

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Main Authors: Farashiani, Mohammad Ebrahim, Muhamad Awang, Rita, Sefidkon, Fatemeh, Omar, Dzolkhifli, Assareh, Mohammad Hassan, Rahmani, Mawardi
Format: Article
Language:English
Published: Marsland Press 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30396/1/Extraction%20methods%20effects%20on%20composition%20and%20toxicity%20of%20Eucalyptus%20essential%20oil.pdf
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author Farashiani, Mohammad Ebrahim
Muhamad Awang, Rita
Sefidkon, Fatemeh
Omar, Dzolkhifli
Assareh, Mohammad Hassan
Rahmani, Mawardi
author_facet Farashiani, Mohammad Ebrahim
Muhamad Awang, Rita
Sefidkon, Fatemeh
Omar, Dzolkhifli
Assareh, Mohammad Hassan
Rahmani, Mawardi
author_sort Farashiani, Mohammad Ebrahim
collection UPM
description The current investigation was designed to determine the influence of three extraction methods on the composition and insecticidal activity of essential oils extracted from Eucalyptus globulus and E. camaldulensis. The evaluated extraction methods were steam-distillation, steam water-distillation and hydro-distillation. Fumigant toxicity of the extracted essential oils was evaluated against Sitophilus oryzae. The GC and GC–MS methods were used for analyzing the oil chemical composition. Both E. globulus and E. camaldulensis showed significant differences in oil yield (w/w, based on dry weight) with direct steam distillation resulting in low oil yields (0.8%; 0.35%) compared to water distillation (2.35%; 2.22%) and water + steam distillation (2.03%; 1. 53%). We identified nineteen compounds in the essential oils of these species. 1, 8-Cineol (27.67-82%), α-Pinene (4.67-8.13%) and Limonene (2.49-10.53%) were the major components of the oils and the highest amount of 1, 8-Cineol (82%) was obtained with water distillation, while steam-distillation resulted in the lowest amount of Cineol (27%). The results of the toxicity study of the essential oils demonstrated that the highest toxicity (LC50 = 24.89 µL/L air) was observed against S. oryzae populations treated with oils extracted by water distillation. In conclusion, the extraction of Eucalyptus essential oils by hydro-distillation had some priorities over the oil extractions by the other methods because hydro-distillation extracted oil had the highest fumigant activity, high and fast-oil yields as well as high percentage composition of 1,8-Cineol.
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spelling upm.eprints-303962018-07-20T08:50:27Z http://psasir.upm.edu.my/id/eprint/30396/ Extraction methods effects on composition and toxicity of Eucalyptus essential oil Farashiani, Mohammad Ebrahim Muhamad Awang, Rita Sefidkon, Fatemeh Omar, Dzolkhifli Assareh, Mohammad Hassan Rahmani, Mawardi The current investigation was designed to determine the influence of three extraction methods on the composition and insecticidal activity of essential oils extracted from Eucalyptus globulus and E. camaldulensis. The evaluated extraction methods were steam-distillation, steam water-distillation and hydro-distillation. Fumigant toxicity of the extracted essential oils was evaluated against Sitophilus oryzae. The GC and GC–MS methods were used for analyzing the oil chemical composition. Both E. globulus and E. camaldulensis showed significant differences in oil yield (w/w, based on dry weight) with direct steam distillation resulting in low oil yields (0.8%; 0.35%) compared to water distillation (2.35%; 2.22%) and water + steam distillation (2.03%; 1. 53%). We identified nineteen compounds in the essential oils of these species. 1, 8-Cineol (27.67-82%), α-Pinene (4.67-8.13%) and Limonene (2.49-10.53%) were the major components of the oils and the highest amount of 1, 8-Cineol (82%) was obtained with water distillation, while steam-distillation resulted in the lowest amount of Cineol (27%). The results of the toxicity study of the essential oils demonstrated that the highest toxicity (LC50 = 24.89 µL/L air) was observed against S. oryzae populations treated with oils extracted by water distillation. In conclusion, the extraction of Eucalyptus essential oils by hydro-distillation had some priorities over the oil extractions by the other methods because hydro-distillation extracted oil had the highest fumigant activity, high and fast-oil yields as well as high percentage composition of 1,8-Cineol. Marsland Press 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30396/1/Extraction%20methods%20effects%20on%20composition%20and%20toxicity%20of%20Eucalyptus%20essential%20oil.pdf Farashiani, Mohammad Ebrahim and Muhamad Awang, Rita and Sefidkon, Fatemeh and Omar, Dzolkhifli and Assareh, Mohammad Hassan and Rahmani, Mawardi (2013) Extraction methods effects on composition and toxicity of Eucalyptus essential oil. Life Science Journal, 10 (4). pp. 2401-2405. ISSN 1097-8135; ESSN: 2372-613X http://www.lifesciencesite.com/lsj/life1004/ 10.7537/marslsj100413.321
spellingShingle Farashiani, Mohammad Ebrahim
Muhamad Awang, Rita
Sefidkon, Fatemeh
Omar, Dzolkhifli
Assareh, Mohammad Hassan
Rahmani, Mawardi
Extraction methods effects on composition and toxicity of Eucalyptus essential oil
title Extraction methods effects on composition and toxicity of Eucalyptus essential oil
title_full Extraction methods effects on composition and toxicity of Eucalyptus essential oil
title_fullStr Extraction methods effects on composition and toxicity of Eucalyptus essential oil
title_full_unstemmed Extraction methods effects on composition and toxicity of Eucalyptus essential oil
title_short Extraction methods effects on composition and toxicity of Eucalyptus essential oil
title_sort extraction methods effects on composition and toxicity of eucalyptus essential oil
url http://psasir.upm.edu.my/id/eprint/30396/1/Extraction%20methods%20effects%20on%20composition%20and%20toxicity%20of%20Eucalyptus%20essential%20oil.pdf
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