Identification polypeptide biomarkers of porcine skin gelatin by two-dimensional electrophoresis
The peptide composition of gelatin is known to vary very common that the electrophoretic pattern of gelatin from one source differs from another source even for the same raw material. Therefore, the present study aimed to use proteomics field to identify gelatin polypeptides biomarker for dependi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30456/1/30456.pdf |
| _version_ | 1825947710700650496 |
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| author | Mohd Amin, Aina Ismail, Amin Raja Nhari, Raja Mohd Hafidz Che Man, Yaakob |
| author_facet | Mohd Amin, Aina Ismail, Amin Raja Nhari, Raja Mohd Hafidz Che Man, Yaakob |
| author_sort | Mohd Amin, Aina |
| collection | UPM |
| description | The peptide composition of gelatin is known to vary very common that the electrophoretic
pattern of gelatin from one source differs from another source even for the same raw material.
Therefore, the present study aimed to use proteomics field to identify gelatin polypeptides
biomarker for depending on the condition under which collagen is hydrolyzed. Hence, it is
porcine skins. The polypeptides obtained for porcine skin gelatins can be used as reference
in future to detect the origins of gelatin added in the processed food. We compared porcine
skin gelatin samples obtained from three producers. Total average numbers of polypeptides
of porcine skin gelatins from company A, B and C were 303 ± 2.8, 285.5 ± 3.5 and 270.5 ±
4.9 spots respectively. 10 biomarkers were identified and presented in all different companies.
We also did a mixture of porcine and bovine skin gelatin to detect the presence of these 10
biomarkers. The level of adulteration that could be detected was as low as 1.0% w/w. |
| first_indexed | 2024-03-06T08:17:37Z |
| format | Article |
| id | upm.eprints-30456 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:17:37Z |
| publishDate | 2013 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-304562015-06-02T06:45:40Z http://psasir.upm.edu.my/id/eprint/30456/ Identification polypeptide biomarkers of porcine skin gelatin by two-dimensional electrophoresis Mohd Amin, Aina Ismail, Amin Raja Nhari, Raja Mohd Hafidz Che Man, Yaakob The peptide composition of gelatin is known to vary very common that the electrophoretic pattern of gelatin from one source differs from another source even for the same raw material. Therefore, the present study aimed to use proteomics field to identify gelatin polypeptides biomarker for depending on the condition under which collagen is hydrolyzed. Hence, it is porcine skins. The polypeptides obtained for porcine skin gelatins can be used as reference in future to detect the origins of gelatin added in the processed food. We compared porcine skin gelatin samples obtained from three producers. Total average numbers of polypeptides of porcine skin gelatins from company A, B and C were 303 ± 2.8, 285.5 ± 3.5 and 270.5 ± 4.9 spots respectively. 10 biomarkers were identified and presented in all different companies. We also did a mixture of porcine and bovine skin gelatin to detect the presence of these 10 biomarkers. The level of adulteration that could be detected was as low as 1.0% w/w. Faculty of Food Science and Technology, Universiti Putra Malaysia 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30456/1/30456.pdf Mohd Amin, Aina and Ismail, Amin and Raja Nhari, Raja Mohd Hafidz and Che Man, Yaakob (2013) Identification polypeptide biomarkers of porcine skin gelatin by two-dimensional electrophoresis. International Food Research Journal, 20 (3). pp. 1395-1399. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/ifrj-2013-20-issue-3.html English |
| spellingShingle | Mohd Amin, Aina Ismail, Amin Raja Nhari, Raja Mohd Hafidz Che Man, Yaakob Identification polypeptide biomarkers of porcine skin gelatin by two-dimensional electrophoresis |
| title | Identification polypeptide biomarkers of porcine skin gelatin by two-dimensional electrophoresis |
| title_full | Identification polypeptide biomarkers of porcine skin gelatin by two-dimensional electrophoresis |
| title_fullStr | Identification polypeptide biomarkers of porcine skin gelatin by two-dimensional electrophoresis |
| title_full_unstemmed | Identification polypeptide biomarkers of porcine skin gelatin by two-dimensional electrophoresis |
| title_short | Identification polypeptide biomarkers of porcine skin gelatin by two-dimensional electrophoresis |
| title_sort | identification polypeptide biomarkers of porcine skin gelatin by two dimensional electrophoresis |
| url | http://psasir.upm.edu.my/id/eprint/30456/1/30456.pdf |
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