| Summary: | An awareness of
Escherichia coli
as a foodborne pathogen and illness causing bacterium has
been increased among consumers. Moreover, there is demand for natural product in order to
reduce synthetic product that can cause toxic to the human. In this study, antibacterial activity,
in term of MIC, MBC and killing-time curve of methanolic extract of
Boesenbergia rotunda
have been tested against a standard
E. coli
ATCC 25922 and two
E. coli
isolated from milk
products using Clinical and Laboratory Standard Institute (CLSI) methods. The results show
that
B. rotunda
extract was susceptible to all
E. coli
strains. The MIC and MBC values of
B.
rotunda
extract against
E. coli
ranged 0.019 mg/mL
2.5 mg/mL and 0.039 mg/mL – 5.0 μg/mL,
respectively. Killing-time curves were constructed at concentrations of 0x MIC, 1/2x MIC, 1x
MIC, and 2x MIC. All
E. coli
strains can be killed with concentration of 2x MIC after 2 hours.
The results show that
B. rotunda
extract has potential antibacterial activity against
E. coli.
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