Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia
Foodborne diseases are mainly caused by bacterial contamination which can lead to severe diarrhea. This study aimed to detect the presence of Shiga toxin-Producing Escherichia coli O157, Escherichia coli non-O157 and virulence gene in raw vegetables. The samples were purchased from wet market...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30525/1/30525.pdf |
| _version_ | 1796971758548418560 |
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| author | Loo, Yuet Ying Soopna, Puspanadan Goh, Sur Guat Kuan, Chee Hao Chang, Wei San Lye, Ying Ling Tang, John Yew Huat Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_facet | Loo, Yuet Ying Soopna, Puspanadan Goh, Sur Guat Kuan, Chee Hao Chang, Wei San Lye, Ying Ling Tang, John Yew Huat Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_sort | Loo, Yuet Ying |
| collection | UPM |
| description | Foodborne diseases are mainly caused by bacterial contamination which can lead to severe
diarrhea. This study aimed to detect the presence of Shiga toxin-Producing
Escherichia coli
O157,
Escherichia coli
non-O157 and virulence gene in raw vegetables. The samples were
purchased from wet market and hypermarket in Selangor. The detections were carried out by
using the combination methods of Most Probable Number-Polymerase Chain Reaction (MPN-
PCR). A total of 37(18.5%) samples were found to be contaminated by STEC. Out of these
37 isolates, four (10.8%) of the isolates were E. coli O157 while 33(89.2%) were
E. coli
non-
O157. However, there was no
E. coli
O157:H7 detected in all the samples. The occurrence of
Shiga toxin-Producing
E. coli
in edible raw vegetables samples suggests the importance of this
pathogen in vegetables. Therefore, more studies are required to remove this pathogen from
vegetables. |
| first_indexed | 2024-03-06T08:17:49Z |
| format | Article |
| id | upm.eprints-30525 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:17:49Z |
| publishDate | 2013 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-305252015-06-02T03:45:35Z http://psasir.upm.edu.my/id/eprint/30525/ Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia Loo, Yuet Ying Soopna, Puspanadan Goh, Sur Guat Kuan, Chee Hao Chang, Wei San Lye, Ying Ling Tang, John Yew Huat Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Foodborne diseases are mainly caused by bacterial contamination which can lead to severe diarrhea. This study aimed to detect the presence of Shiga toxin-Producing Escherichia coli O157, Escherichia coli non-O157 and virulence gene in raw vegetables. The samples were purchased from wet market and hypermarket in Selangor. The detections were carried out by using the combination methods of Most Probable Number-Polymerase Chain Reaction (MPN- PCR). A total of 37(18.5%) samples were found to be contaminated by STEC. Out of these 37 isolates, four (10.8%) of the isolates were E. coli O157 while 33(89.2%) were E. coli non- O157. However, there was no E. coli O157:H7 detected in all the samples. The occurrence of Shiga toxin-Producing E. coli in edible raw vegetables samples suggests the importance of this pathogen in vegetables. Therefore, more studies are required to remove this pathogen from vegetables. Faculty of Food Science and Technology, Universiti Putra Malaysia 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30525/1/30525.pdf Loo, Yuet Ying and Soopna, Puspanadan and Goh, Sur Guat and Kuan, Chee Hao and Chang, Wei San and Lye, Ying Ling and Tang, John Yew Huat and Rukayadi, Yaya and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2013) Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia. International Food Research Journal, 20 (6). 3313 - 3317. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-20-2013.html |
| spellingShingle | Loo, Yuet Ying Soopna, Puspanadan Goh, Sur Guat Kuan, Chee Hao Chang, Wei San Lye, Ying Ling Tang, John Yew Huat Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia |
| title | Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia |
| title_full | Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia |
| title_fullStr | Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia |
| title_full_unstemmed | Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia |
| title_short | Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia |
| title_sort | quantitative detection and characterization of shiga toxin producing escherichia coli o157 and non o157 in raw vegetables by mpn pcr in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/30525/1/30525.pdf |
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