Antagonistic activities of endophytic bacteria against Fusarium wilt of black pepper (Piper nigrum).
Fusarium wilt is a fungal disease, which affects a broad range of plants including black pepper (Piper nigrum). Fusarium solani f. sp. piperis is a common causal agent of root rots and stem blight in black pepper. F. oxysporum Schl. f. sp. piperis, a less common but an important pathogen of black pe...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Friends Science Publishers
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/30714/1/Antagonistic%20activities%20of%20endophytic%20bacteria%20against%20Fusarium%20wilt%20of%20black%20pepper.pdf |
| _version_ | 1796971796559298560 |
|---|---|
| author | Edward, Edkona Jenang Wong, Sing King Leong, Stephen Chan Teck Jiwan, Make Ab. Aziz, Zakry Fitri Kundat, Franklin Ragai Ahmed, Osumanu Haruna Nik Ab. Majid, Nik Muhamad |
| author_facet | Edward, Edkona Jenang Wong, Sing King Leong, Stephen Chan Teck Jiwan, Make Ab. Aziz, Zakry Fitri Kundat, Franklin Ragai Ahmed, Osumanu Haruna Nik Ab. Majid, Nik Muhamad |
| author_sort | Edward, Edkona Jenang |
| collection | UPM |
| description | Fusarium wilt is a fungal disease, which affects a broad range of plants including black pepper (Piper nigrum). Fusarium solani f. sp. piperis is a common causal agent of root rots and stem blight in black pepper. F. oxysporum Schl. f. sp. piperis, a less common but an important pathogen of black pepper. The biological approach to control F. oxysporum is becoming popular in many crop plants however there is lack of scientific record in black pepper. Endophytic bacteria were isolated from black pepper roots and cultured on nutrient agar. The bacterial isolates were screened for in vitro antagonistic activity against F. oxysporum through dual culture, mycelial growth, spore germination and double plate tests. Five isolates with promising antifungal activity were further identified through 16S rDNA sequencing. Isolates EB1 and EB2 showed highest antagonism against F. oxysporum mycelia with the percentage of inhibition up to 43% and 41%, respectively. Isolated EB3, EB4 and EB5 produced clearing zones in spore germination test with radii measurements at 12.5-15.0 mm. The antifungal activities apparently involved the secretion of volatile and diffusible bioactive compounds. Analysis of the 16S rDNA sequences suggested the closest identities of the bacterial isolates as Bacillus megaterium, Bacillus cereus, Enterobacter sp. and Bacillus sp. Five endophytic bacteria isolates demonstrated significant control over both mycelia growth and spore germination of F. oxysporum. Some of these bacteria might possess additional beneficial plant growth promoting and insecticidal properties for the development of multi-function products in black pepper farming. |
| first_indexed | 2024-03-06T08:18:24Z |
| format | Article |
| id | upm.eprints-30714 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:18:24Z |
| publishDate | 2013 |
| publisher | Friends Science Publishers |
| record_format | dspace |
| spelling | upm.eprints-307142015-09-09T01:02:49Z http://psasir.upm.edu.my/id/eprint/30714/ Antagonistic activities of endophytic bacteria against Fusarium wilt of black pepper (Piper nigrum). Edward, Edkona Jenang Wong, Sing King Leong, Stephen Chan Teck Jiwan, Make Ab. Aziz, Zakry Fitri Kundat, Franklin Ragai Ahmed, Osumanu Haruna Nik Ab. Majid, Nik Muhamad Fusarium wilt is a fungal disease, which affects a broad range of plants including black pepper (Piper nigrum). Fusarium solani f. sp. piperis is a common causal agent of root rots and stem blight in black pepper. F. oxysporum Schl. f. sp. piperis, a less common but an important pathogen of black pepper. The biological approach to control F. oxysporum is becoming popular in many crop plants however there is lack of scientific record in black pepper. Endophytic bacteria were isolated from black pepper roots and cultured on nutrient agar. The bacterial isolates were screened for in vitro antagonistic activity against F. oxysporum through dual culture, mycelial growth, spore germination and double plate tests. Five isolates with promising antifungal activity were further identified through 16S rDNA sequencing. Isolates EB1 and EB2 showed highest antagonism against F. oxysporum mycelia with the percentage of inhibition up to 43% and 41%, respectively. Isolated EB3, EB4 and EB5 produced clearing zones in spore germination test with radii measurements at 12.5-15.0 mm. The antifungal activities apparently involved the secretion of volatile and diffusible bioactive compounds. Analysis of the 16S rDNA sequences suggested the closest identities of the bacterial isolates as Bacillus megaterium, Bacillus cereus, Enterobacter sp. and Bacillus sp. Five endophytic bacteria isolates demonstrated significant control over both mycelia growth and spore germination of F. oxysporum. Some of these bacteria might possess additional beneficial plant growth promoting and insecticidal properties for the development of multi-function products in black pepper farming. Friends Science Publishers 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30714/1/Antagonistic%20activities%20of%20endophytic%20bacteria%20against%20Fusarium%20wilt%20of%20black%20pepper.pdf Edward, Edkona Jenang and Wong, Sing King and Leong, Stephen Chan Teck and Jiwan, Make and Ab. Aziz, Zakry Fitri and Kundat, Franklin Ragai and Ahmed, Osumanu Haruna and Nik Ab. Majid, Nik Muhamad (2013) Antagonistic activities of endophytic bacteria against Fusarium wilt of black pepper (Piper nigrum). International Journal of Agriculture and Biology, 15 (2). pp. 291-296. ISSN 1560-8530; ESSN: 1814-9596 |
| spellingShingle | Edward, Edkona Jenang Wong, Sing King Leong, Stephen Chan Teck Jiwan, Make Ab. Aziz, Zakry Fitri Kundat, Franklin Ragai Ahmed, Osumanu Haruna Nik Ab. Majid, Nik Muhamad Antagonistic activities of endophytic bacteria against Fusarium wilt of black pepper (Piper nigrum). |
| title | Antagonistic activities of endophytic bacteria against Fusarium wilt of black pepper (Piper nigrum). |
| title_full | Antagonistic activities of endophytic bacteria against Fusarium wilt of black pepper (Piper nigrum). |
| title_fullStr | Antagonistic activities of endophytic bacteria against Fusarium wilt of black pepper (Piper nigrum). |
| title_full_unstemmed | Antagonistic activities of endophytic bacteria against Fusarium wilt of black pepper (Piper nigrum). |
| title_short | Antagonistic activities of endophytic bacteria against Fusarium wilt of black pepper (Piper nigrum). |
| title_sort | antagonistic activities of endophytic bacteria against fusarium wilt of black pepper piper nigrum |
| url | http://psasir.upm.edu.my/id/eprint/30714/1/Antagonistic%20activities%20of%20endophytic%20bacteria%20against%20Fusarium%20wilt%20of%20black%20pepper.pdf |
| work_keys_str_mv | AT edwardedkonajenang antagonisticactivitiesofendophyticbacteriaagainstfusariumwiltofblackpepperpipernigrum AT wongsingking antagonisticactivitiesofendophyticbacteriaagainstfusariumwiltofblackpepperpipernigrum AT leongstephenchanteck antagonisticactivitiesofendophyticbacteriaagainstfusariumwiltofblackpepperpipernigrum AT jiwanmake antagonisticactivitiesofendophyticbacteriaagainstfusariumwiltofblackpepperpipernigrum AT abazizzakryfitri antagonisticactivitiesofendophyticbacteriaagainstfusariumwiltofblackpepperpipernigrum AT kundatfranklinragai antagonisticactivitiesofendophyticbacteriaagainstfusariumwiltofblackpepperpipernigrum AT ahmedosumanuharuna antagonisticactivitiesofendophyticbacteriaagainstfusariumwiltofblackpepperpipernigrum AT nikabmajidnikmuhamad antagonisticactivitiesofendophyticbacteriaagainstfusariumwiltofblackpepperpipernigrum |