Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology
Response surface methodology (RSM) was used to optimize the co-production of a mixture of crude cellulosic and hemicellulosic enzymes (endoglucanase, xylanase, and mannanase) by Aspergillus terreus K1 in solid-state fermentation (SSF) using palm kernel expeller (PKE) as the sole carbon source. These...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
North Carolina State University
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/30724/1/Optimization%20of%20multi.pdf |
| _version_ | 1796971798586195968 |
|---|---|
| author | Chen, Wei Li Liang, Juan Boo Jahromi, Mohammad Faseleh Ho, Yin Wan Abdullah, Norhani |
| author_facet | Chen, Wei Li Liang, Juan Boo Jahromi, Mohammad Faseleh Ho, Yin Wan Abdullah, Norhani |
| author_sort | Chen, Wei Li |
| collection | UPM |
| description | Response surface methodology (RSM) was used to optimize the co-production of a mixture of crude cellulosic and hemicellulosic enzymes (endoglucanase, xylanase, and mannanase) by Aspergillus terreus K1 in solid-state fermentation (SSF) using palm kernel expeller (PKE) as the sole carbon source. These enzymes have gained renewed interest due to their efficacy to improve the digestibility of PKE for use in diets of mono-gastric animals (poultry, pigs, and fish). The results showed that temperature, moisture, inoculum concentration, and initial pH had significant (P< 0.05) effects on the enzymes production. Using PKE as a solid substrate, maximum endoglucanase, mannanase, and xylanase (17.37, 41.24, and 265.57 U/g DM, respectively) were obtained at 30.5 °C, 62.7% moisture, 6% inoculum, and pH 5.8. The enzyme activities recorded were close to the predicted values (19.97, 44.12, and 262.01 U/g DM, respectively). |
| first_indexed | 2024-03-06T08:18:25Z |
| format | Article |
| id | upm.eprints-30724 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:18:25Z |
| publishDate | 2013 |
| publisher | North Carolina State University |
| record_format | dspace |
| spelling | upm.eprints-307242020-02-07T01:07:20Z http://psasir.upm.edu.my/id/eprint/30724/ Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology Chen, Wei Li Liang, Juan Boo Jahromi, Mohammad Faseleh Ho, Yin Wan Abdullah, Norhani Response surface methodology (RSM) was used to optimize the co-production of a mixture of crude cellulosic and hemicellulosic enzymes (endoglucanase, xylanase, and mannanase) by Aspergillus terreus K1 in solid-state fermentation (SSF) using palm kernel expeller (PKE) as the sole carbon source. These enzymes have gained renewed interest due to their efficacy to improve the digestibility of PKE for use in diets of mono-gastric animals (poultry, pigs, and fish). The results showed that temperature, moisture, inoculum concentration, and initial pH had significant (P< 0.05) effects on the enzymes production. Using PKE as a solid substrate, maximum endoglucanase, mannanase, and xylanase (17.37, 41.24, and 265.57 U/g DM, respectively) were obtained at 30.5 °C, 62.7% moisture, 6% inoculum, and pH 5.8. The enzyme activities recorded were close to the predicted values (19.97, 44.12, and 262.01 U/g DM, respectively). North Carolina State University 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30724/1/Optimization%20of%20multi.pdf Chen, Wei Li and Liang, Juan Boo and Jahromi, Mohammad Faseleh and Ho, Yin Wan and Abdullah, Norhani (2013) Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology. BioResources, 8 (3). pp. 3844-3857. ISSN 1930-2126 http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_08_3_3844_Chen_Optimization_Fungi_Isolated |
| spellingShingle | Chen, Wei Li Liang, Juan Boo Jahromi, Mohammad Faseleh Ho, Yin Wan Abdullah, Norhani Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology |
| title | Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology |
| title_full | Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology |
| title_fullStr | Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology |
| title_full_unstemmed | Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology |
| title_short | Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology |
| title_sort | optimization of multi enzyme production by fungi isolated from palm kernel expeller using response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/30724/1/Optimization%20of%20multi.pdf |
| work_keys_str_mv | AT chenweili optimizationofmultienzymeproductionbyfungiisolatedfrompalmkernelexpellerusingresponsesurfacemethodology AT liangjuanboo optimizationofmultienzymeproductionbyfungiisolatedfrompalmkernelexpellerusingresponsesurfacemethodology AT jahromimohammadfaseleh optimizationofmultienzymeproductionbyfungiisolatedfrompalmkernelexpellerusingresponsesurfacemethodology AT hoyinwan optimizationofmultienzymeproductionbyfungiisolatedfrompalmkernelexpellerusingresponsesurfacemethodology AT abdullahnorhani optimizationofmultienzymeproductionbyfungiisolatedfrompalmkernelexpellerusingresponsesurfacemethodology |