Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel
Plant peels could be a potential source of novel pectinases for use in various industrial applications due to their broad substrate specificity with high stability under extreme conditions. Therefore, the extraction conditions of a novel pectinase enzyme from pitaya peel was optimized in this study....
| Główni autorzy: | , |
|---|---|
| Format: | Artykuł |
| Język: | English |
| Wydane: |
MDPI
2013
|
| Dostęp online: | http://psasir.upm.edu.my/id/eprint/30827/1/Optimization%20of%20extraction%20of%20novel%20pectinase%20enzyme%20discovered%20in%20red%20pitaya.pdf |
| _version_ | 1825947784398766080 |
|---|---|
| author | Ahmad Zohdi, Nor Khanani Amid, Mehrnoush |
| author_facet | Ahmad Zohdi, Nor Khanani Amid, Mehrnoush |
| author_sort | Ahmad Zohdi, Nor Khanani |
| collection | UPM |
| description | Plant peels could be a potential source of novel pectinases for use in various industrial applications due to their broad substrate specificity with high stability under extreme conditions. Therefore, the extraction conditions of a novel pectinase enzyme from pitaya peel was optimized in this study. The effect of extraction variables, namely buffer to sample ratio (2:1 to 8:1, X1), extraction temperature (−15 to +25 °C, X2) and buffer pH (4.0 to 12.0, X3) on specific activity, temperature stability, storage stability and surfactant agent stability of pectinase from pitaya peel was investigated. The study demonstrated that the optimum conditions for the extraction of pectinase from pitaya sources could improve the enzymatic characteristics of the enzyme and protect its activity and stability during the extraction procedure. The optimum extraction conditions cause the pectinase to achieve high specific activity (15.31 U/mg), temperature stability (78%), storage stability (88%) and surfactant agent stability (83%). The most desirable conditions to achieve the highest activity and stability of pectinase enzyme from pitaya peel were the use of 5:1 buffer to sample ratio at 5 °C and pH 8.0. |
| first_indexed | 2024-03-06T08:18:44Z |
| format | Article |
| id | upm.eprints-30827 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:18:44Z |
| publishDate | 2013 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-308272016-12-23T08:09:14Z http://psasir.upm.edu.my/id/eprint/30827/ Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel Ahmad Zohdi, Nor Khanani Amid, Mehrnoush Plant peels could be a potential source of novel pectinases for use in various industrial applications due to their broad substrate specificity with high stability under extreme conditions. Therefore, the extraction conditions of a novel pectinase enzyme from pitaya peel was optimized in this study. The effect of extraction variables, namely buffer to sample ratio (2:1 to 8:1, X1), extraction temperature (−15 to +25 °C, X2) and buffer pH (4.0 to 12.0, X3) on specific activity, temperature stability, storage stability and surfactant agent stability of pectinase from pitaya peel was investigated. The study demonstrated that the optimum conditions for the extraction of pectinase from pitaya sources could improve the enzymatic characteristics of the enzyme and protect its activity and stability during the extraction procedure. The optimum extraction conditions cause the pectinase to achieve high specific activity (15.31 U/mg), temperature stability (78%), storage stability (88%) and surfactant agent stability (83%). The most desirable conditions to achieve the highest activity and stability of pectinase enzyme from pitaya peel were the use of 5:1 buffer to sample ratio at 5 °C and pH 8.0. MDPI 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30827/1/Optimization%20of%20extraction%20of%20novel%20pectinase%20enzyme%20discovered%20in%20red%20pitaya.pdf Ahmad Zohdi, Nor Khanani and Amid, Mehrnoush (2013) Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel. Molecules, 18 (11). pp. 14366-14380. ISSN 1420-3049 http://www.mdpi.com/1420-3049/18/11/14366 10.3390/molecules181114366 |
| spellingShingle | Ahmad Zohdi, Nor Khanani Amid, Mehrnoush Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel |
| title | Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel |
| title_full | Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel |
| title_fullStr | Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel |
| title_full_unstemmed | Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel |
| title_short | Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel |
| title_sort | optimization of extraction of novel pectinase enzyme discovered in red pitaya hylocereus polyrhizus peel |
| url | http://psasir.upm.edu.my/id/eprint/30827/1/Optimization%20of%20extraction%20of%20novel%20pectinase%20enzyme%20discovered%20in%20red%20pitaya.pdf |
| work_keys_str_mv | AT ahmadzohdinorkhanani optimizationofextractionofnovelpectinaseenzymediscoveredinredpitayahylocereuspolyrhizuspeel AT amidmehrnoush optimizationofextractionofnovelpectinaseenzymediscoveredinredpitayahylocereuspolyrhizuspeel |