Optimization and validation of a method for extraction and quantification of ochratoxin A in black pepper
The extraction method for the determination of ochratoxin A (OTA) in black pepper was optimized. The influence of three variables, i.e., type of solvent, solvent-volume-to-sample-size ratio (v/w) and amount of sodium chloride (NaCl) (g), on OTA recovery was evaluated. Analysis of variance was used t...
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30879/1/%289%29.pdf |
| _version_ | 1825947794647547904 |
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| author | Jalili, Maryam Selamat, Jinap |
| author_facet | Jalili, Maryam Selamat, Jinap |
| author_sort | Jalili, Maryam |
| collection | UPM |
| description | The extraction method for the determination of ochratoxin A (OTA) in black pepper was optimized. The influence of three variables, i.e., type of solvent, solvent-volume-to-sample-size ratio (v/w) and amount of sodium chloride (NaCl) (g), on OTA recovery was evaluated. Analysis of variance was used to compare recovery values obtained from different solvents, and response surface methodology (RSM) was used to determine the optimum amount of NaCl and the solvent-volume-to-sample-size ratio. The concentration of OTA was determined by high-performance liquid chromatography with fluorescence detection. The highest recovery (95.2 %) was obtained when methanol/water (80:20, v/v) was used as the solvent. The RSM results showed that the experimental data could be adequately fitted to a second-order polynomial model with multiple regression coefficients (R2) of 0.962. The optimum amount of NaCl was determined to be 3 g, whereas the optimum solvent-volume-to-sample-size ratio (v/w) was found to be 4. The proposed method was applied to 20 samples, and the presence of OTA was found in 8 (40%) samples ranging from 0.11 to 3.16 ng g-1. |
| first_indexed | 2024-03-06T08:18:53Z |
| format | Article |
| id | upm.eprints-30879 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:18:53Z |
| publishDate | 2015 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-308792016-01-27T01:09:41Z http://psasir.upm.edu.my/id/eprint/30879/ Optimization and validation of a method for extraction and quantification of ochratoxin A in black pepper Jalili, Maryam Selamat, Jinap The extraction method for the determination of ochratoxin A (OTA) in black pepper was optimized. The influence of three variables, i.e., type of solvent, solvent-volume-to-sample-size ratio (v/w) and amount of sodium chloride (NaCl) (g), on OTA recovery was evaluated. Analysis of variance was used to compare recovery values obtained from different solvents, and response surface methodology (RSM) was used to determine the optimum amount of NaCl and the solvent-volume-to-sample-size ratio. The concentration of OTA was determined by high-performance liquid chromatography with fluorescence detection. The highest recovery (95.2 %) was obtained when methanol/water (80:20, v/v) was used as the solvent. The RSM results showed that the experimental data could be adequately fitted to a second-order polynomial model with multiple regression coefficients (R2) of 0.962. The optimum amount of NaCl was determined to be 3 g, whereas the optimum solvent-volume-to-sample-size ratio (v/w) was found to be 4. The proposed method was applied to 20 samples, and the presence of OTA was found in 8 (40%) samples ranging from 0.11 to 3.16 ng g-1. Faculty of Food Science and Technology, Universiti Putra Malaysia 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30879/1/%289%29.pdf Jalili, Maryam and Selamat, Jinap (2015) Optimization and validation of a method for extraction and quantification of ochratoxin A in black pepper. International Food Research Journal, 22 (2). pp. 502-509. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/22%20%2802%29%202015/%289%29.pdf |
| spellingShingle | Jalili, Maryam Selamat, Jinap Optimization and validation of a method for extraction and quantification of ochratoxin A in black pepper |
| title | Optimization and validation of a method for extraction and quantification of ochratoxin A in black pepper |
| title_full | Optimization and validation of a method for extraction and quantification of ochratoxin A in black pepper |
| title_fullStr | Optimization and validation of a method for extraction and quantification of ochratoxin A in black pepper |
| title_full_unstemmed | Optimization and validation of a method for extraction and quantification of ochratoxin A in black pepper |
| title_short | Optimization and validation of a method for extraction and quantification of ochratoxin A in black pepper |
| title_sort | optimization and validation of a method for extraction and quantification of ochratoxin a in black pepper |
| url | http://psasir.upm.edu.my/id/eprint/30879/1/%289%29.pdf |
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