A Spectrophotometric Method for the Determination of Glucose with Glucose Oxidase [E.C. 1.11.1.7] Using Titanium (IV)-4-(2'-pyridylazo)Resorcinol Reagent
A mixture of Ti(IV) and 4-(2-pyridylazo) resorcinol was found to be useful in the spectrophotometric determination of trace amounts of hydrogen peroxide. The absorbance of the complex formed at 508 nm was proportional to the concentration of hydrogen peroxide added. The reagent was applied to the as...
Main Authors: | , , |
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Format: | Article |
Language: | English English |
Published: |
Universiti Putra Malaysia Press
1994
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Online Access: | http://psasir.upm.edu.my/id/eprint/3107/1/A_Spectrophotometric_Method_for_the_Determination_of.pdf |
Summary: | A mixture of Ti(IV) and 4-(2-pyridylazo) resorcinol was found to be useful in the spectrophotometric determination of trace amounts of hydrogen peroxide. The absorbance of the complex formed at 508 nm was proportional to the concentration of hydrogen peroxide added. The reagent was applied to the assay of glucose through coupling with glucose oxidase which produces HP2' This method of glucose determination was rapid, convenient and showed minimal interference
from reducible substances. |
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