Identification of bioactive compounds produced by bacteria-based biopesticides, Bacillus thuringiensis ATCC 10792 produced in shrimp pond sludge
Biological controls of destructive pest in forest and agriculture sector using bacteria were proven to minimize the problems caused by the usage of chemical insecticides. However, their application is limited due to high production cost through fermentation. In this study, shrimp pond sludge and was...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Department of Biology, Faculty of Science, Universiti Putra Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/31190/1/BIO%202014-11.pdf |
| _version_ | 1825947856994828288 |
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| author | Ulul Azmi, Noormasshela Abdul Ghafar, Nurul Shaziera Cheong, Jee Yin Yakubu, Safiya Ahmad Adli, Azleen Abd Aziz, Nor Azwady Mustafa, Muskhazli |
| author_facet | Ulul Azmi, Noormasshela Abdul Ghafar, Nurul Shaziera Cheong, Jee Yin Yakubu, Safiya Ahmad Adli, Azleen Abd Aziz, Nor Azwady Mustafa, Muskhazli |
| author_sort | Ulul Azmi, Noormasshela |
| collection | UPM |
| description | Biological controls of destructive pest in forest and agriculture sector using bacteria were proven to minimize the problems caused by the usage of chemical insecticides. However, their application is limited due to high production cost through fermentation. In this study, shrimp pond sludge and wastewater were used as an alternate culture medium for bacteria-based biopesticides production. It has been determined by previous part of this study that Bacillus thuringiensis ATCC 10792 in hydrolysed shrimp pond has showed the highest potential to be used as bacteria-based biopestecides based on their high growth rate (7.44x106 ± 5.16 CFU/ml) and sporulation (5.90x106 ± 7.88 CFU/ml). Bioassay of entomotoxicity also showed high mortality rate, disruption on the target insect physically and affect the life cycle of the target insect. Hence, proteomic analysis using SDS-Page and MALDITOF-MS were conducted to identify the bioactive compound that contribute to the toxicity of the bacteria-based biopesticides produced. Separation and identification of the bioactive components produced by the bacteria during the fermentation were done and two types of protein were produced by the bacteria. Superoxide dismustase and spore coat protein were identified to be produced during the fermentation and respectively play an important roles to the toxicity of the bacteria. This study showed the potential of Bt ATCC10792 to be used as biopesticides. |
| first_indexed | 2024-03-06T08:19:50Z |
| format | Conference or Workshop Item |
| id | upm.eprints-31190 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:19:50Z |
| publishDate | 2014 |
| publisher | Department of Biology, Faculty of Science, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-311902020-04-14T14:45:56Z http://psasir.upm.edu.my/id/eprint/31190/ Identification of bioactive compounds produced by bacteria-based biopesticides, Bacillus thuringiensis ATCC 10792 produced in shrimp pond sludge Ulul Azmi, Noormasshela Abdul Ghafar, Nurul Shaziera Cheong, Jee Yin Yakubu, Safiya Ahmad Adli, Azleen Abd Aziz, Nor Azwady Mustafa, Muskhazli Biological controls of destructive pest in forest and agriculture sector using bacteria were proven to minimize the problems caused by the usage of chemical insecticides. However, their application is limited due to high production cost through fermentation. In this study, shrimp pond sludge and wastewater were used as an alternate culture medium for bacteria-based biopesticides production. It has been determined by previous part of this study that Bacillus thuringiensis ATCC 10792 in hydrolysed shrimp pond has showed the highest potential to be used as bacteria-based biopestecides based on their high growth rate (7.44x106 ± 5.16 CFU/ml) and sporulation (5.90x106 ± 7.88 CFU/ml). Bioassay of entomotoxicity also showed high mortality rate, disruption on the target insect physically and affect the life cycle of the target insect. Hence, proteomic analysis using SDS-Page and MALDITOF-MS were conducted to identify the bioactive compound that contribute to the toxicity of the bacteria-based biopesticides produced. Separation and identification of the bioactive components produced by the bacteria during the fermentation were done and two types of protein were produced by the bacteria. Superoxide dismustase and spore coat protein were identified to be produced during the fermentation and respectively play an important roles to the toxicity of the bacteria. This study showed the potential of Bt ATCC10792 to be used as biopesticides. Department of Biology, Faculty of Science, Universiti Putra Malaysia 2014 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/31190/1/BIO%202014-11.pdf Ulul Azmi, Noormasshela and Abdul Ghafar, Nurul Shaziera and Cheong, Jee Yin and Yakubu, Safiya and Ahmad Adli, Azleen and Abd Aziz, Nor Azwady and Mustafa, Muskhazli (2014) Identification of bioactive compounds produced by bacteria-based biopesticides, Bacillus thuringiensis ATCC 10792 produced in shrimp pond sludge. In: Malaysia International Biological Symposium 2014, 28-29 Oct. 2014, Putrajaya, Malaysia. (p. 88). |
| spellingShingle | Ulul Azmi, Noormasshela Abdul Ghafar, Nurul Shaziera Cheong, Jee Yin Yakubu, Safiya Ahmad Adli, Azleen Abd Aziz, Nor Azwady Mustafa, Muskhazli Identification of bioactive compounds produced by bacteria-based biopesticides, Bacillus thuringiensis ATCC 10792 produced in shrimp pond sludge |
| title | Identification of bioactive compounds produced by bacteria-based biopesticides, Bacillus thuringiensis ATCC 10792 produced in shrimp pond sludge |
| title_full | Identification of bioactive compounds produced by bacteria-based biopesticides, Bacillus thuringiensis ATCC 10792 produced in shrimp pond sludge |
| title_fullStr | Identification of bioactive compounds produced by bacteria-based biopesticides, Bacillus thuringiensis ATCC 10792 produced in shrimp pond sludge |
| title_full_unstemmed | Identification of bioactive compounds produced by bacteria-based biopesticides, Bacillus thuringiensis ATCC 10792 produced in shrimp pond sludge |
| title_short | Identification of bioactive compounds produced by bacteria-based biopesticides, Bacillus thuringiensis ATCC 10792 produced in shrimp pond sludge |
| title_sort | identification of bioactive compounds produced by bacteria based biopesticides bacillus thuringiensis atcc 10792 produced in shrimp pond sludge |
| url | http://psasir.upm.edu.my/id/eprint/31190/1/BIO%202014-11.pdf |
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