Identification and quantification of marine siderophores using high-performance liquid chromatography with electrospray ionization mass spectrometry
Siderophores are low-molecular-weight organic compounds (500 - 1500 Da) produced by heterotrophic bacteria and highly selective for Fe(III). These complexation of iron by siderophores has the potential to affect both the solubility and bioavailability of iron (III) in seawater. We have determined th...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Department of Biology, Faculty of Science, Universiti Putra Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/31200/1/BIO%202014-21.pdf |
| _version_ | 1796971891909459968 |
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| author | Mohamed @ Mohd Ramli, Khairul Nizam Gledhill, Martha |
| author_facet | Mohamed @ Mohd Ramli, Khairul Nizam Gledhill, Martha |
| author_sort | Mohamed @ Mohd Ramli, Khairul Nizam |
| collection | UPM |
| description | Siderophores are low-molecular-weight organic compounds (500 - 1500 Da) produced by heterotrophic bacteria and highly selective for Fe(III). These complexation of iron by siderophores has the potential to affect both the solubility and bioavailability of iron (III) in seawater. We have determined the ferrioxamine siderophores in the seawater samples from high latitude North Atlantic Ocean The identification and quantification of this siderophore type chelate was done by using recently developed high-performance liquid chromatography-mass spectrometry methods. Five different siderophore type chelates were detected and the compounds comprised of two groups; ferrioxamines and amphibactins group. In the dissolved phase, three types of hydroxamate siderophore have been identified; Ferioxamine B (FOB), Ferrioxamine G (FOG) and ferrioxamine E (FOE). Concentration of dissolved FOB, FOG and FOE are extremely low between 0–135 x 10-18 M. Our present data indicated the presence of low concentrations of dissolved siderophores in the high latitude of the Atlantic Ocean and suggest that siderophore distributions are both spatially and temporally variable. |
| first_indexed | 2024-03-06T08:19:52Z |
| format | Conference or Workshop Item |
| id | upm.eprints-31200 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:19:52Z |
| publishDate | 2014 |
| publisher | Department of Biology, Faculty of Science, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-312002020-04-14T14:44:23Z http://psasir.upm.edu.my/id/eprint/31200/ Identification and quantification of marine siderophores using high-performance liquid chromatography with electrospray ionization mass spectrometry Mohamed @ Mohd Ramli, Khairul Nizam Gledhill, Martha Siderophores are low-molecular-weight organic compounds (500 - 1500 Da) produced by heterotrophic bacteria and highly selective for Fe(III). These complexation of iron by siderophores has the potential to affect both the solubility and bioavailability of iron (III) in seawater. We have determined the ferrioxamine siderophores in the seawater samples from high latitude North Atlantic Ocean The identification and quantification of this siderophore type chelate was done by using recently developed high-performance liquid chromatography-mass spectrometry methods. Five different siderophore type chelates were detected and the compounds comprised of two groups; ferrioxamines and amphibactins group. In the dissolved phase, three types of hydroxamate siderophore have been identified; Ferioxamine B (FOB), Ferrioxamine G (FOG) and ferrioxamine E (FOE). Concentration of dissolved FOB, FOG and FOE are extremely low between 0–135 x 10-18 M. Our present data indicated the presence of low concentrations of dissolved siderophores in the high latitude of the Atlantic Ocean and suggest that siderophore distributions are both spatially and temporally variable. Department of Biology, Faculty of Science, Universiti Putra Malaysia 2014 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/31200/1/BIO%202014-21.pdf Mohamed @ Mohd Ramli, Khairul Nizam and Gledhill, Martha (2014) Identification and quantification of marine siderophores using high-performance liquid chromatography with electrospray ionization mass spectrometry. In: Malaysia International Biological Symposium 2014, 28-29 Oct. 2014, Putrajaya, Malaysia. (p. 119). |
| spellingShingle | Mohamed @ Mohd Ramli, Khairul Nizam Gledhill, Martha Identification and quantification of marine siderophores using high-performance liquid chromatography with electrospray ionization mass spectrometry |
| title | Identification and quantification of marine siderophores using high-performance liquid chromatography with electrospray ionization mass spectrometry |
| title_full | Identification and quantification of marine siderophores using high-performance liquid chromatography with electrospray ionization mass spectrometry |
| title_fullStr | Identification and quantification of marine siderophores using high-performance liquid chromatography with electrospray ionization mass spectrometry |
| title_full_unstemmed | Identification and quantification of marine siderophores using high-performance liquid chromatography with electrospray ionization mass spectrometry |
| title_short | Identification and quantification of marine siderophores using high-performance liquid chromatography with electrospray ionization mass spectrometry |
| title_sort | identification and quantification of marine siderophores using high performance liquid chromatography with electrospray ionization mass spectrometry |
| url | http://psasir.upm.edu.my/id/eprint/31200/1/BIO%202014-21.pdf |
| work_keys_str_mv | AT mohamedmohdramlikhairulnizam identificationandquantificationofmarinesiderophoresusinghighperformanceliquidchromatographywithelectrosprayionizationmassspectrometry AT gledhillmartha identificationandquantificationofmarinesiderophoresusinghighperformanceliquidchromatographywithelectrosprayionizationmassspectrometry |