Incidence of fern contamination in nodal segment cultures of Shorea parvifolia dyer
The greatest drawback in large scale micropropagation of tropical woody forest species is high contamination of cultures. In developing a sterilization protocol for micropropagation of Shorea parvifolia Dyer utilizing nodal segments excised from nursery-grown seedlings, it was found that washing 20%...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/31354/1/6.%20Incidence%20of%20Fern%20Contamination%20in%20Nodal%20Segment%20Cultures.pdf |
| _version_ | 1825947887609053184 |
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| author | Mohd Yusof, Aziah Ab Shukor, Nor Aini Awang, Kamis Abdul Kadir, Mihdzar |
| author_facet | Mohd Yusof, Aziah Ab Shukor, Nor Aini Awang, Kamis Abdul Kadir, Mihdzar |
| author_sort | Mohd Yusof, Aziah |
| collection | UPM |
| description | The greatest drawback in large scale micropropagation of tropical woody forest species is high contamination of cultures. In developing a sterilization protocol for micropropagation of Shorea parvifolia Dyer utilizing nodal segments excised from nursery-grown seedlings, it was found that washing 20% (v/v) with Clorox solution for 18 minutes was the best. After six weeks of culture in WPM media supplemented with 10-5 M BAP (apart from fungal and bacterial contamination), the nodal segments developed hair-like structures which were amenable to subculture. Upon subculture, green leafy structures developed from the mass of hairy structure after six weeks. These later developed into ferns which are normally found as epiphytes on older forest trees, known as Asplenium nidus. |
| first_indexed | 2024-03-06T08:20:18Z |
| format | Article |
| id | upm.eprints-31354 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:20:18Z |
| publishDate | 2013 |
| publisher | Universiti Putra Malaysia Press |
| record_format | dspace |
| spelling | upm.eprints-313542015-11-25T03:41:37Z http://psasir.upm.edu.my/id/eprint/31354/ Incidence of fern contamination in nodal segment cultures of Shorea parvifolia dyer Mohd Yusof, Aziah Ab Shukor, Nor Aini Awang, Kamis Abdul Kadir, Mihdzar The greatest drawback in large scale micropropagation of tropical woody forest species is high contamination of cultures. In developing a sterilization protocol for micropropagation of Shorea parvifolia Dyer utilizing nodal segments excised from nursery-grown seedlings, it was found that washing 20% (v/v) with Clorox solution for 18 minutes was the best. After six weeks of culture in WPM media supplemented with 10-5 M BAP (apart from fungal and bacterial contamination), the nodal segments developed hair-like structures which were amenable to subculture. Upon subculture, green leafy structures developed from the mass of hairy structure after six weeks. These later developed into ferns which are normally found as epiphytes on older forest trees, known as Asplenium nidus. Universiti Putra Malaysia Press 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/31354/1/6.%20Incidence%20of%20Fern%20Contamination%20in%20Nodal%20Segment%20Cultures.pdf Mohd Yusof, Aziah and Ab Shukor, Nor Aini and Awang, Kamis and Abdul Kadir, Mihdzar (2013) Incidence of fern contamination in nodal segment cultures of Shorea parvifolia dyer. Pertanika Journal of Tropical Agricultural Science, 36 (S). pp. 67-78. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2036%20(S)%20Dec.%202013/07%20Page%2067-78%20(JTAS%200462-2012).pdf |
| spellingShingle | Mohd Yusof, Aziah Ab Shukor, Nor Aini Awang, Kamis Abdul Kadir, Mihdzar Incidence of fern contamination in nodal segment cultures of Shorea parvifolia dyer |
| title | Incidence of fern contamination in nodal segment cultures of Shorea parvifolia dyer |
| title_full | Incidence of fern contamination in nodal segment cultures of Shorea parvifolia dyer |
| title_fullStr | Incidence of fern contamination in nodal segment cultures of Shorea parvifolia dyer |
| title_full_unstemmed | Incidence of fern contamination in nodal segment cultures of Shorea parvifolia dyer |
| title_short | Incidence of fern contamination in nodal segment cultures of Shorea parvifolia dyer |
| title_sort | incidence of fern contamination in nodal segment cultures of shorea parvifolia dyer |
| url | http://psasir.upm.edu.my/id/eprint/31354/1/6.%20Incidence%20of%20Fern%20Contamination%20in%20Nodal%20Segment%20Cultures.pdf |
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