Molecular survey of Ehrlichia canis in blood and ticks collected from stray dogs in Kuala Lumpur, Malaysia

Ehrlichia canis is the etiological agent of canine monocytic ehrlichiosis, a potentially fatal disease of dogs and is transmitted by the brown dog tick, Rhipicephalus sanguineus. Recent studies have detected other possible tick vectors. Thus far, only one study has been carried out to determine th...

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Main Authors: Anurddin, Siti Hawa, Watanabe, Malaika
Format: Conference or Workshop Item
Language:English
Published: 2010
Online Access:http://psasir.upm.edu.my/id/eprint/31666/1/Proceedings-58.pdf
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author Anurddin, Siti Hawa
Watanabe, Malaika
author_facet Anurddin, Siti Hawa
Watanabe, Malaika
author_sort Anurddin, Siti Hawa
collection UPM
description Ehrlichia canis is the etiological agent of canine monocytic ehrlichiosis, a potentially fatal disease of dogs and is transmitted by the brown dog tick, Rhipicephalus sanguineus. Recent studies have detected other possible tick vectors. Thus far, only one study has been carried out to determine the prevalence of E.canis using molecular methods and there are as yet no study to that detects the organism in ticks in Malaysia. Polymerase chain reaction (PCR) is known to be a sensitive and specific method for the diagnosis of canine ehrlichiosis and currently is the diagnostic method of choice worldwide. Polymerase chain reaction was performed using a species-specific set of primers for the detection of E.canis in blood and ticks collected from 60 stray dogs from Dewan Bandaraya Kuala Lumpur. Out of a total of 122 ticks, 120 were identified as Rhipicephalus sanguineus and two were identified as Haemaphysalis sp. E.canis DNA could not be amplified from any of the 60 canine blood samples or the 60 tick samples.
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spelling upm.eprints-316662014-11-06T10:22:32Z http://psasir.upm.edu.my/id/eprint/31666/ Molecular survey of Ehrlichia canis in blood and ticks collected from stray dogs in Kuala Lumpur, Malaysia Anurddin, Siti Hawa Watanabe, Malaika Ehrlichia canis is the etiological agent of canine monocytic ehrlichiosis, a potentially fatal disease of dogs and is transmitted by the brown dog tick, Rhipicephalus sanguineus. Recent studies have detected other possible tick vectors. Thus far, only one study has been carried out to determine the prevalence of E.canis using molecular methods and there are as yet no study to that detects the organism in ticks in Malaysia. Polymerase chain reaction (PCR) is known to be a sensitive and specific method for the diagnosis of canine ehrlichiosis and currently is the diagnostic method of choice worldwide. Polymerase chain reaction was performed using a species-specific set of primers for the detection of E.canis in blood and ticks collected from 60 stray dogs from Dewan Bandaraya Kuala Lumpur. Out of a total of 122 ticks, 120 were identified as Rhipicephalus sanguineus and two were identified as Haemaphysalis sp. E.canis DNA could not be amplified from any of the 60 canine blood samples or the 60 tick samples. 2010-01-05 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/31666/1/Proceedings-58.pdf Anurddin, Siti Hawa and Watanabe, Malaika (2010) Molecular survey of Ehrlichia canis in blood and ticks collected from stray dogs in Kuala Lumpur, Malaysia. In: 5th Seminar on Veterinary Sciences, 5-8 Jan. 2010, Serdang, Selangor. .
spellingShingle Anurddin, Siti Hawa
Watanabe, Malaika
Molecular survey of Ehrlichia canis in blood and ticks collected from stray dogs in Kuala Lumpur, Malaysia
title Molecular survey of Ehrlichia canis in blood and ticks collected from stray dogs in Kuala Lumpur, Malaysia
title_full Molecular survey of Ehrlichia canis in blood and ticks collected from stray dogs in Kuala Lumpur, Malaysia
title_fullStr Molecular survey of Ehrlichia canis in blood and ticks collected from stray dogs in Kuala Lumpur, Malaysia
title_full_unstemmed Molecular survey of Ehrlichia canis in blood and ticks collected from stray dogs in Kuala Lumpur, Malaysia
title_short Molecular survey of Ehrlichia canis in blood and ticks collected from stray dogs in Kuala Lumpur, Malaysia
title_sort molecular survey of ehrlichia canis in blood and ticks collected from stray dogs in kuala lumpur malaysia
url http://psasir.upm.edu.my/id/eprint/31666/1/Proceedings-58.pdf
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