Distribution of serotypes, penicillin susceptibilities and virulence genes in Streptococcus pneumoniae isolates from Malaysian healthy children
This study was carried out to analyze the distribution of serotypes, penicillin susceptibilities, and virulence genes among nasal carriage of Streptococcus pneumoniaefrom Malaysian healthy children. Fifty isolates were obtained from nasal swabs of children aged 5 years and younger who attended 3-da...
| Main Authors: | , , , , , |
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| 格式: | Conference or Workshop Item |
| 语言: | English |
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2012
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| 在线阅读: | http://psasir.upm.edu.my/id/eprint/31768/1/31768.pdf |
| _version_ | 1825947964691972096 |
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| author | Mohamed Shakrin, Nik Noorul Shakira Mohd Yatim, Masura Masri, Siti Norbaya Mohd Taib, Niazlin Jamal, Farida Fatema @ Farida Mohd Desa, Mohd Nasir |
| author_facet | Mohamed Shakrin, Nik Noorul Shakira Mohd Yatim, Masura Masri, Siti Norbaya Mohd Taib, Niazlin Jamal, Farida Fatema @ Farida Mohd Desa, Mohd Nasir |
| author_sort | Mohamed Shakrin, Nik Noorul Shakira |
| collection | UPM |
| description | This study was carried out to analyze the distribution of serotypes, penicillin susceptibilities, and virulence genes
among nasal carriage of Streptococcus pneumoniaefrom Malaysian healthy children. Fifty isolates were obtained from nasal swabs of children aged 5 years and younger who attended 3-day care centers in Kuala Lumpur, Malaysia from
June to December 2010. The isolates were serotyped using commercial antisera. Minimum inhibitory concentrations
(MICs) for penicillin of the isolates were then determined by E test followed by polymerase chain reaction (PCR)-
screening for 8 virulence (LytA, Ply, PsaA, PspA, cbp A, cbp G, PavA & rlr) and 3 penicillin-binding protein (pbp La,
2b and 2x) genes. The later were further subjected to restriction-fragment-Iength-polymorphism (RFLP)analysis and
compared against the penicillin susceptibility of the isolates. Among the 50 isolates, 11 serotypes were identified with serotype 6A been the most common, 2 isolates were non-typeable. 28 (56%) were penicillin susceptible (PSSP), 6 (12%) were intermediate (PISP) and 16 (32%) were resistant (PRSP).PRSPwas high among serotypes 6A (68.75%) followed by 23F (25%) and non-typeable (6.25%). The 8 virulence genes were detected in all isolates except for rlr gene; 8 (16%). PCR-RFLPanalvsis showed different pbp-gene patterns among the PRSPs which is consistent with
the altered pbp-genes. Therefore, this study indicates that, with such phenotypic and genotypic pattern, the nasal
isolates have potential to institute infection among this high group of the population.
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| first_indexed | 2024-03-06T08:21:28Z |
| format | Conference or Workshop Item |
| id | upm.eprints-31768 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:21:28Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | upm.eprints-317682015-01-22T01:33:37Z http://psasir.upm.edu.my/id/eprint/31768/ Distribution of serotypes, penicillin susceptibilities and virulence genes in Streptococcus pneumoniae isolates from Malaysian healthy children Mohamed Shakrin, Nik Noorul Shakira Mohd Yatim, Masura Masri, Siti Norbaya Mohd Taib, Niazlin Jamal, Farida Fatema @ Farida Mohd Desa, Mohd Nasir This study was carried out to analyze the distribution of serotypes, penicillin susceptibilities, and virulence genes among nasal carriage of Streptococcus pneumoniaefrom Malaysian healthy children. Fifty isolates were obtained from nasal swabs of children aged 5 years and younger who attended 3-day care centers in Kuala Lumpur, Malaysia from June to December 2010. The isolates were serotyped using commercial antisera. Minimum inhibitory concentrations (MICs) for penicillin of the isolates were then determined by E test followed by polymerase chain reaction (PCR)- screening for 8 virulence (LytA, Ply, PsaA, PspA, cbp A, cbp G, PavA & rlr) and 3 penicillin-binding protein (pbp La, 2b and 2x) genes. The later were further subjected to restriction-fragment-Iength-polymorphism (RFLP)analysis and compared against the penicillin susceptibility of the isolates. Among the 50 isolates, 11 serotypes were identified with serotype 6A been the most common, 2 isolates were non-typeable. 28 (56%) were penicillin susceptible (PSSP), 6 (12%) were intermediate (PISP) and 16 (32%) were resistant (PRSP).PRSPwas high among serotypes 6A (68.75%) followed by 23F (25%) and non-typeable (6.25%). The 8 virulence genes were detected in all isolates except for rlr gene; 8 (16%). PCR-RFLPanalvsis showed different pbp-gene patterns among the PRSPs which is consistent with the altered pbp-genes. Therefore, this study indicates that, with such phenotypic and genotypic pattern, the nasal isolates have potential to institute infection among this high group of the population. 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/31768/1/31768.pdf Mohamed Shakrin, Nik Noorul Shakira and Mohd Yatim, Masura and Masri, Siti Norbaya and Mohd Taib, Niazlin and Jamal, Farida Fatema @ Farida and Mohd Desa, Mohd Nasir (2012) Distribution of serotypes, penicillin susceptibilities and virulence genes in Streptococcus pneumoniae isolates from Malaysian healthy children. In: International Conference On One Health & 24th VAM Congress 2012 , 21-23 Sep. 2012, Marriott Putrajaya, Malaysia. (pp. 40-42). |
| spellingShingle | Mohamed Shakrin, Nik Noorul Shakira Mohd Yatim, Masura Masri, Siti Norbaya Mohd Taib, Niazlin Jamal, Farida Fatema @ Farida Mohd Desa, Mohd Nasir Distribution of serotypes, penicillin susceptibilities and virulence genes in Streptococcus pneumoniae isolates from Malaysian healthy children |
| title | Distribution of serotypes, penicillin susceptibilities and virulence genes in Streptococcus pneumoniae isolates from Malaysian healthy children |
| title_full | Distribution of serotypes, penicillin susceptibilities and virulence genes in Streptococcus pneumoniae isolates from Malaysian healthy children |
| title_fullStr | Distribution of serotypes, penicillin susceptibilities and virulence genes in Streptococcus pneumoniae isolates from Malaysian healthy children |
| title_full_unstemmed | Distribution of serotypes, penicillin susceptibilities and virulence genes in Streptococcus pneumoniae isolates from Malaysian healthy children |
| title_short | Distribution of serotypes, penicillin susceptibilities and virulence genes in Streptococcus pneumoniae isolates from Malaysian healthy children |
| title_sort | distribution of serotypes penicillin susceptibilities and virulence genes in streptococcus pneumoniae isolates from malaysian healthy children |
| url | http://psasir.upm.edu.my/id/eprint/31768/1/31768.pdf |
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