The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids
Latex agglutination test (L) and a modification of the latex test, protein A-coated latex (PAL) were compared for sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested against purified CyMV. The minimum detectable virus concentration was sim...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Universiti Putra Malaysia Press
1993
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| Online Access: | http://psasir.upm.edu.my/id/eprint/3186/1/The_Use_of_Antibody-sensitized_Latex_to_Detect_Cymbidium_Mosaic.pdf |
| Summary: | Latex agglutination test (L) and a modification of the latex test, protein A-coated latex (PAL) were compared for
sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested
against purified CyMV. The minimum detectable virus concentration was similar for both L and PAL reactions
at 0.104 llg/ml. The detectable end points in infected Oncidium Gower Ramsey orchid sap were similar at a
dilution of 1/2560. PAL was found to be more sensitive than L at a higher globulin dilution of 1/512. The PAL
test showed higher sensitivity than L based on five orchid hybrids and compared favourably with electron
microscopy test. |
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