The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids
Latex agglutination test (L) and a modification of the latex test, protein A-coated latex (PAL) were compared for sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested against purified CyMV. The minimum detectable virus concentration was sim...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
1993
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| Online Access: | http://psasir.upm.edu.my/id/eprint/3186/1/The_Use_of_Antibody-sensitized_Latex_to_Detect_Cymbidium_Mosaic.pdf |
| _version_ | 1825921609772302336 |
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| author | Abdul Samad, Norani Ari, Zainab |
| author_facet | Abdul Samad, Norani Ari, Zainab |
| author_sort | Abdul Samad, Norani |
| collection | UPM |
| description | Latex agglutination test (L) and a modification of the latex test, protein A-coated latex (PAL) were compared for
sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested
against purified CyMV. The minimum detectable virus concentration was similar for both L and PAL reactions
at 0.104 llg/ml. The detectable end points in infected Oncidium Gower Ramsey orchid sap were similar at a
dilution of 1/2560. PAL was found to be more sensitive than L at a higher globulin dilution of 1/512. The PAL
test showed higher sensitivity than L based on five orchid hybrids and compared favourably with electron
microscopy test. |
| first_indexed | 2024-03-06T07:00:24Z |
| format | Article |
| id | upm.eprints-3186 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:00:24Z |
| publishDate | 1993 |
| publisher | Universiti Putra Malaysia Press |
| record_format | dspace |
| spelling | upm.eprints-31862015-09-01T03:40:57Z http://psasir.upm.edu.my/id/eprint/3186/ The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids Abdul Samad, Norani Ari, Zainab Latex agglutination test (L) and a modification of the latex test, protein A-coated latex (PAL) were compared for sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested against purified CyMV. The minimum detectable virus concentration was similar for both L and PAL reactions at 0.104 llg/ml. The detectable end points in infected Oncidium Gower Ramsey orchid sap were similar at a dilution of 1/2560. PAL was found to be more sensitive than L at a higher globulin dilution of 1/512. The PAL test showed higher sensitivity than L based on five orchid hybrids and compared favourably with electron microscopy test. Universiti Putra Malaysia Press 1993 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3186/1/The_Use_of_Antibody-sensitized_Latex_to_Detect_Cymbidium_Mosaic.pdf Abdul Samad, Norani and Ari, Zainab (1993) The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids. Pertanika Journal of Tropical Agricultural Science, 16 (2). pp. 157-160. ISSN 0126-6128 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2016%20(2)%20Aug.%201993/13%20JTAS%20Vol.16%20(2)%201993%20(Pg%20157-160).pdf |
| spellingShingle | Abdul Samad, Norani Ari, Zainab The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids |
| title | The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids |
| title_full | The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids |
| title_fullStr | The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids |
| title_full_unstemmed | The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids |
| title_short | The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids |
| title_sort | use of antibody sensitized latex to detect cymbidium mosaic virus in orchids |
| url | http://psasir.upm.edu.my/id/eprint/3186/1/The_Use_of_Antibody-sensitized_Latex_to_Detect_Cymbidium_Mosaic.pdf |
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