Enhancement of enzymatic properties of T1 lipase by saturation mutagenesis at glutamine 114
Rational design of the recombinant T1 lipase gene by saturation mutagenesis on the oxyanion Gln114 was successfully carried out to afford a library of twenty lipase variants. The objective of the study was to investigate the impact of a single point saturation mutagenesis at the oxyanion Gln114 on t...
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| Format: | Thesis |
| Language: | English |
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2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/31933/1/FS%202012%2035R.pdf |