A method for detecting candida species in a specimen by polymerase chain reaction.
The present invention relates to a method for detecting Candida species in a specimen, comprising the steps of extracting nucleic acid from the specimen; performing polymerase chain reaction on the extracted nucleic acid using DNA primers for amplification of targeted nucleic acid sequence; detectin...
| Main Authors: | , , , |
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| Format: | Patent |
| Language: | English |
| Published: |
2011
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| Summary: | The present invention relates to a method for detecting Candida species in a specimen, comprising the steps of extracting nucleic acid from the specimen; performing polymerase chain reaction on the extracted nucleic acid using DNA primers for amplification of targeted nucleic acid sequence; detecting the amplified nucleic acid for each Candida species; characterized in that, in a single reaction, the DNA primers consist of: SEQ ID NO. 1 and SEQ ID NO. 2 or their complements for amplifying nucleic acid from Candida albicans; SEQ ID NO.3 and SEQ ID NO. 4 or their complements for amplifying nucleic acid from Candida glabrata; SEQ ID NO. 5 and SEQ ID NO. 6 or their complements for amplifying nucleic acid from Candida parapsilosis; SEQ ID NO. 7 and SEQ ID NO. 8 or their complements for amplifying nucleic acid from Candida tropicalis. |
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