A method for detecting candida species in a specimen by polymerase chain reaction.
The present invention relates to a method for detecting Candida species in a specimen, comprising the steps of extracting nucleic acid from the specimen; performing polymerase chain reaction on the extracted nucleic acid using DNA primers for amplification of targeted nucleic acid sequence; detectin...
| Main Authors: | , , , |
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| Format: | Patent |
| Language: | English |
| Published: |
2011
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| _version_ | 1796972063590711296 |
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| author | Chong, Pei Pei Mohammed Harmal, Nabil Saad Jamal, Farida Sekawi, Zamberi |
| author_facet | Chong, Pei Pei Mohammed Harmal, Nabil Saad Jamal, Farida Sekawi, Zamberi |
| author_sort | Chong, Pei Pei |
| collection | UPM |
| description | The present invention relates to a method for detecting Candida species in a specimen, comprising the steps of extracting nucleic acid from the specimen; performing polymerase chain reaction on the extracted nucleic acid using DNA primers for amplification of targeted nucleic acid sequence; detecting the amplified nucleic acid for each Candida species; characterized in that, in a single reaction, the DNA primers consist of: SEQ ID NO. 1 and SEQ ID NO. 2 or their complements for amplifying nucleic acid from Candida albicans; SEQ ID NO.3 and SEQ ID NO. 4 or their complements for amplifying nucleic acid from Candida glabrata; SEQ ID NO. 5 and SEQ ID NO. 6 or their complements for amplifying nucleic acid from Candida parapsilosis; SEQ ID NO. 7 and SEQ ID NO. 8 or their complements for amplifying nucleic acid from Candida tropicalis. |
| first_indexed | 2024-03-06T08:22:20Z |
| format | Patent |
| id | upm.eprints-32082 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:22:20Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | upm.eprints-320822014-10-15T08:18:05Z http://psasir.upm.edu.my/id/eprint/32082/ A method for detecting candida species in a specimen by polymerase chain reaction. Chong, Pei Pei Mohammed Harmal, Nabil Saad Jamal, Farida Sekawi, Zamberi The present invention relates to a method for detecting Candida species in a specimen, comprising the steps of extracting nucleic acid from the specimen; performing polymerase chain reaction on the extracted nucleic acid using DNA primers for amplification of targeted nucleic acid sequence; detecting the amplified nucleic acid for each Candida species; characterized in that, in a single reaction, the DNA primers consist of: SEQ ID NO. 1 and SEQ ID NO. 2 or their complements for amplifying nucleic acid from Candida albicans; SEQ ID NO.3 and SEQ ID NO. 4 or their complements for amplifying nucleic acid from Candida glabrata; SEQ ID NO. 5 and SEQ ID NO. 6 or their complements for amplifying nucleic acid from Candida parapsilosis; SEQ ID NO. 7 and SEQ ID NO. 8 or their complements for amplifying nucleic acid from Candida tropicalis. 2011-02-11 Patent NonPeerReviewed Chong Pei Pei (2011) A method for detecting candida species in a specimen by polymerase chain reaction. PI2011000631. English |
| spellingShingle | Chong, Pei Pei Mohammed Harmal, Nabil Saad Jamal, Farida Sekawi, Zamberi A method for detecting candida species in a specimen by polymerase chain reaction. |
| title | A method for detecting candida species in a specimen by polymerase chain reaction.
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| title_full | A method for detecting candida species in a specimen by polymerase chain reaction.
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| title_fullStr | A method for detecting candida species in a specimen by polymerase chain reaction.
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| title_full_unstemmed | A method for detecting candida species in a specimen by polymerase chain reaction.
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| title_short | A method for detecting candida species in a specimen by polymerase chain reaction.
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| title_sort | method for detecting candida species in a specimen by polymerase chain reaction |
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