Effect of Dissolved Oxygen on the Growth, Sporulation and Larvicidal Activity of Bacillus sphaericus 2362
Bacillus sphaericus produces bacterial protein toxin capable of killing larvae of mosquitoes which spread diseases such as filariasis, encephalitis and malaria. In batch culture, a maximum spore count of 2.9 x 109/ml media was obtained after 48 h cultivation. In continuous culture, a dilution rate...
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| Format: | Article |
| Language: | English English |
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Universiti Putra Malaysia Press
1996
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| Online Access: | http://psasir.upm.edu.my/id/eprint/3217/1/Effect_of_Dissolved_Oxygen_on_the_Growth%2C_Sporulation.pdf |
| Summary: | Bacillus sphaericus produces bacterial protein toxin capable of killing larvae of mosquitoes which spread diseases such as filariasis, encephalitis and
malaria. In batch culture, a maximum spore count of 2.9 x 109/ml media was obtained after 48 h cultivation. In continuous culture, a dilution rate
of 0.10 hr- I was found to give a higher spore count than 0.15 h- 1 and 0.2 h- I dilution rates. Kinetic studies on dissolved oxygen (DO) levels showed that
20,50 and 100% gave rapid formation of free spores, whilst 5% DO gave
poor results. Cells grown at 5, 20 and 50% DO levels gave comparable
values of mosquito-larvicidal activity while those grown at 100% DO were
significantly less toxic. |
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