Isolation and development of DNA microsatellite markers for the river catfish (Mystus nemurus)
The microsatellite markers for Mystus nemurus were isolated using the 5’ anchored PCR procedure. A degenerate primer was designed for the construction of the genomic library. Fourteen clones were sequenced and revealed a total of 45 microsatellite repeats. From these, 20 specific primer pairs wer...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Asian Fisheries Society
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/32536/1/56.%20Isolation%20and%20Development%20of%20DNA%20Microsatellite%20Markers%20for%20the%20River%20Catfish%20%28Mystus%20nemurus%29.pdf |
| Summary: | The microsatellite markers for Mystus nemurus were isolated using the 5’ anchored
PCR procedure. A degenerate primer was designed for the construction of the
genomic library. Fourteen clones were sequenced and revealed a total of 45 microsatellite
repeats. From these, 20 specific primer pairs were designed and employed to characterize
unrelated fishes from six populations of M. nemurus. Thirteen loci were found to be
polymorphic. The allelic frequencies ranged from 0.007 to 0.800, while the levels of
heterozygosity ranged from 0.0563 (MnBp5-1-115b) to 0.8714 (MnBp5-1-12a). |
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