Organic solvent tolerant lipases
The nature of the enzymes will be denatured in the presence of the organic solvents. Solvents not only affect enzyme stability but also change enzyme specificity. Most of the reports have been published concerning solvent stable enzymes. However, these enzymes are not naturally solvent stable enzyme...
| Main Authors: | , , , , |
|---|---|
| Format: | Book Section |
| Language: | English |
| Published: |
Nova Science Publishers
2006
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/32975/1/Abstract-Organic%20solvent%20tolerant%20lipases.pdf |
| _version_ | 1796972228199317504 |
|---|---|
| author | Baharum, Syarul Nataqain Sulong, Mohamad Ropaning Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_facet | Baharum, Syarul Nataqain Sulong, Mohamad Ropaning Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_sort | Baharum, Syarul Nataqain |
| collection | UPM |
| description | The nature of the enzymes will be denatured in the presence of the organic solvents. Solvents not only affect enzyme stability but also change enzyme specificity. Most of the reports have been published concerning solvent stable enzymes. However, these enzymes are not naturally solvent stable enzymes that direct attempts from screening of organic solvent tolerant bacteria. In this chapter, we review the production and characterization of organic solvent tolerant lipases on the first attemps to screen organic solvent tolerant lipases from Bacillus sphaericus 205y, Bacillus sp. stain 42 and Pseudomonas sp. S5. All of these bacteria were proven to be organic solvent tolerant lipase producers. These lipases were stable in the organic solvents with log P value between 2.0 to 3.6. Organic solvent tolerant lipases from Pseudomonas sp. S5, B. sphaericus 205y, Bacillus sp. strain 42 were successfully purified to homogeneity. The optimum temperatures of these lipases were 45◦C, 55◦C and 70◦C, respectively. These purified lipases exhibited great stability and high activity in most of the organic solvents tested, therefore, these valuable criteria should not be neglected as important products in different fields could be developed from these lipases. |
| first_indexed | 2024-03-06T08:24:45Z |
| format | Book Section |
| id | upm.eprints-32975 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:24:45Z |
| publishDate | 2006 |
| publisher | Nova Science Publishers |
| record_format | dspace |
| spelling | upm.eprints-329752016-09-26T04:05:36Z http://psasir.upm.edu.my/id/eprint/32975/ Organic solvent tolerant lipases Baharum, Syarul Nataqain Sulong, Mohamad Ropaning Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran The nature of the enzymes will be denatured in the presence of the organic solvents. Solvents not only affect enzyme stability but also change enzyme specificity. Most of the reports have been published concerning solvent stable enzymes. However, these enzymes are not naturally solvent stable enzymes that direct attempts from screening of organic solvent tolerant bacteria. In this chapter, we review the production and characterization of organic solvent tolerant lipases on the first attemps to screen organic solvent tolerant lipases from Bacillus sphaericus 205y, Bacillus sp. stain 42 and Pseudomonas sp. S5. All of these bacteria were proven to be organic solvent tolerant lipase producers. These lipases were stable in the organic solvents with log P value between 2.0 to 3.6. Organic solvent tolerant lipases from Pseudomonas sp. S5, B. sphaericus 205y, Bacillus sp. strain 42 were successfully purified to homogeneity. The optimum temperatures of these lipases were 45◦C, 55◦C and 70◦C, respectively. These purified lipases exhibited great stability and high activity in most of the organic solvents tested, therefore, these valuable criteria should not be neglected as important products in different fields could be developed from these lipases. Nova Science Publishers 2006 Book Section PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/32975/1/Abstract-Organic%20solvent%20tolerant%20lipases.pdf Baharum, Syarul Nataqain and Sulong, Mohamad Ropaning and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2006) Organic solvent tolerant lipases. In: New Lipases and Proteases. Nova Science Publishers, New York, pp. 63-76. ISBN 9781600210686 |
| spellingShingle | Baharum, Syarul Nataqain Sulong, Mohamad Ropaning Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Organic solvent tolerant lipases |
| title | Organic solvent tolerant lipases |
| title_full | Organic solvent tolerant lipases |
| title_fullStr | Organic solvent tolerant lipases |
| title_full_unstemmed | Organic solvent tolerant lipases |
| title_short | Organic solvent tolerant lipases |
| title_sort | organic solvent tolerant lipases |
| url | http://psasir.upm.edu.my/id/eprint/32975/1/Abstract-Organic%20solvent%20tolerant%20lipases.pdf |
| work_keys_str_mv | AT baharumsyarulnataqain organicsolventtolerantlipases AT sulongmohamadropaning organicsolventtolerantlipases AT rajaabdulrahmanrajanoorzaliha organicsolventtolerantlipases AT sallehabubakar organicsolventtolerantlipases AT basrimahiran organicsolventtolerantlipases |