Multiplex assay for detection of Bario rice samples

The present invention provides a four-tube multiplex assay for distinguishing Bario rice from non-Bario rice varieties, said assay comprising the steps of running multiplex PCR with sample using one or more loci of Table 1 and distinguishing the Bario rice from non-Bario rice varieties on the basis...

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Bibliographic Details
Main Authors: Yiu, Pang Hung, Lee, Huei Hong, Wong, Sie Chuong, Amartalingam, Rajan
Format: Patent
Published: 2011
Description
Summary:The present invention provides a four-tube multiplex assay for distinguishing Bario rice from non-Bario rice varieties, said assay comprising the steps of running multiplex PCR with sample using one or more loci of Table 1 and distinguishing the Bario rice from non-Bario rice varieties on the basis of varietal specific multiplex allele profile. The present invention also provides a kit for performing multiplex assay for distinguishing Bario rice from non-Bario rice varieties, which may comprise primers directed to SSR loci, appropriate reagents for PCR and a control DNA template of Bario rice.