Purified T1 lipase, a novel construct of T1 lipase and methods thereoF
The present invention relates to method to substitute second step affinity chromatography with ion exchange chromatography by site-directed mutagenesis at downstream region of GST sequences (H215R and G213R). This is to enable to diferentiate the isoelectric point (pI) value of GST from the pI value...
Main Authors: | Raja Abdul Rahman, Raja Noor Zaliha, Saleh, Abu Bakar, Basri, Mahiran, Leow, Adam Thean Chor, Che Hussi, Che Haznie Ayu |
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Format: | Patent |
Published: |
2011
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