Membrane-mediated control of hepatic JJ-hydroxy-fJ-methylglutarylcoenzyme A reductase
Previously we [Sabine & James (1976) life Sci. 18, 1185-11921 proposed that the activity of hepatic β -hydroxy- β ·methylglutaryl-coenzyme A reductase is critically regulated by the fluidity of its supporting microsomal membrane'. In the present work we examined further this concept of memb...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Portland Press
1981
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| Online Access: | http://psasir.upm.edu.my/id/eprint/33818/1/33818%20membrane-mediated.pdf |
| _version_ | 1796972386960015360 |
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| author | Sipat, Abdullah Sabine, John R. |
| author_facet | Sipat, Abdullah Sabine, John R. |
| author_sort | Sipat, Abdullah |
| collection | UPM |
| description | Previously we [Sabine & James (1976) life Sci. 18, 1185-11921 proposed that the activity of hepatic β -hydroxy- β ·methylglutaryl-coenzyme A reductase is critically regulated by the fluidity of its supporting microsomal membrane'. In the present work we examined further this concept of membrane mediated control, with respect to the specific hypothesis that such control might Junction as a common mechanism both for the co-ordinated regulation of other enzymes affected by cholesterol feeding and also for the subcellular integration of the several physiological factors known to influence this enzyme's activity. Contrary to earlier expectations, this hypothesis now appears not to hold. We report here that, under those conditions of short-term cholesterol feeding that affected the reductase, a variety of other microsomal enzymes did not display membrane-function interactions, i.e. neither enzymes involved in cholesterol metabolism and also affected by cholesterol feeding (cholesterol 7α·hydroxylase), nor those involved in cholesterol metabolism and not affected by cholesterol feeding (hydroxymethylglutaryl-Co A hydrolase, acyl-CoA: cholesterol acyltransfernse). nor those not directly involved in cholesterol metabolism at all (glucose 6-phosohatase). Furthermore, we oberved no evidence for the operation of membrane-mediated control of the reductase in other situations known to influence its activity, i.e. starvation, diurnal rhythm, the very early stages of cholesterol feeding and various manipulations in vitro, |
| first_indexed | 2024-03-06T08:27:15Z |
| format | Article |
| id | upm.eprints-33818 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:27:15Z |
| publishDate | 1981 |
| publisher | Portland Press |
| record_format | dspace |
| spelling | upm.eprints-338182015-04-13T03:38:10Z http://psasir.upm.edu.my/id/eprint/33818/ Membrane-mediated control of hepatic JJ-hydroxy-fJ-methylglutarylcoenzyme A reductase Sipat, Abdullah Sabine, John R. Previously we [Sabine & James (1976) life Sci. 18, 1185-11921 proposed that the activity of hepatic β -hydroxy- β ·methylglutaryl-coenzyme A reductase is critically regulated by the fluidity of its supporting microsomal membrane'. In the present work we examined further this concept of membrane mediated control, with respect to the specific hypothesis that such control might Junction as a common mechanism both for the co-ordinated regulation of other enzymes affected by cholesterol feeding and also for the subcellular integration of the several physiological factors known to influence this enzyme's activity. Contrary to earlier expectations, this hypothesis now appears not to hold. We report here that, under those conditions of short-term cholesterol feeding that affected the reductase, a variety of other microsomal enzymes did not display membrane-function interactions, i.e. neither enzymes involved in cholesterol metabolism and also affected by cholesterol feeding (cholesterol 7α·hydroxylase), nor those involved in cholesterol metabolism and not affected by cholesterol feeding (hydroxymethylglutaryl-Co A hydrolase, acyl-CoA: cholesterol acyltransfernse). nor those not directly involved in cholesterol metabolism at all (glucose 6-phosohatase). Furthermore, we oberved no evidence for the operation of membrane-mediated control of the reductase in other situations known to influence its activity, i.e. starvation, diurnal rhythm, the very early stages of cholesterol feeding and various manipulations in vitro, Portland Press 1981 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/33818/1/33818%20membrane-mediated.pdf Sipat, Abdullah and Sabine, John R. (1981) Membrane-mediated control of hepatic JJ-hydroxy-fJ-methylglutarylcoenzyme A reductase. Biochemical Journal, 194 (3). pp. 889-893. ISSN 0264-6021, ESSN: 1470-8728 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1162825/ |
| spellingShingle | Sipat, Abdullah Sabine, John R. Membrane-mediated control of hepatic JJ-hydroxy-fJ-methylglutarylcoenzyme A reductase |
| title | Membrane-mediated control of hepatic JJ-hydroxy-fJ-methylglutarylcoenzyme A reductase |
| title_full | Membrane-mediated control of hepatic JJ-hydroxy-fJ-methylglutarylcoenzyme A reductase |
| title_fullStr | Membrane-mediated control of hepatic JJ-hydroxy-fJ-methylglutarylcoenzyme A reductase |
| title_full_unstemmed | Membrane-mediated control of hepatic JJ-hydroxy-fJ-methylglutarylcoenzyme A reductase |
| title_short | Membrane-mediated control of hepatic JJ-hydroxy-fJ-methylglutarylcoenzyme A reductase |
| title_sort | membrane mediated control of hepatic jj hydroxy fj methylglutarylcoenzyme a reductase |
| url | http://psasir.upm.edu.my/id/eprint/33818/1/33818%20membrane-mediated.pdf |
| work_keys_str_mv | AT sipatabdullah membranemediatedcontrolofhepaticjjhydroxyfjmethylglutarylcoenzymeareductase AT sabinejohnr membranemediatedcontrolofhepaticjjhydroxyfjmethylglutarylcoenzymeareductase |