Screening of tropical medicinal plants for sporicidal activity

Bacterial spores have special significance in foods because they are much more resistant to physical and chemical antimicrobial treatment. Nowadays, there is interest in using natural products such as plant extract for food preservation. In this study, 26 of tropical medicinal plants and spices were...

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Main Authors: Lau, Kah Yan, Rukayadi, Yaya
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2015
Online Access:http://psasir.upm.edu.my/id/eprint/33877/1/%2861%29.pdf
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author Lau, Kah Yan
Rukayadi, Yaya
author_facet Lau, Kah Yan
Rukayadi, Yaya
author_sort Lau, Kah Yan
collection UPM
description Bacterial spores have special significance in foods because they are much more resistant to physical and chemical antimicrobial treatment. Nowadays, there is interest in using natural products such as plant extract for food preservation. In this study, 26 of tropical medicinal plants and spices were screened for their sporicidal activity against the spores of Bacillus cereus. The spores of B. cereus was harvested after incubation at 30°C for 1 week and treated with various plant extracts using the method of Standard Operating Procedure for the AOAC (Association of Official Analytical Chemists) Sporicidal Activity. Glutaraldehyde was used as a positive control. Among them, Indonesian bay leaf (Eugenia polyantha Wight) inactivated more than 3 log of spores/ml of B. cereus (99.99%) at the concentration of 1% and completely killed B. cereus spores at concentration of 2.5%. These results suggest that Indonesian bay leaf extract has strong sporicidal activity against spores of B. cereus.
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spelling upm.eprints-338772016-01-27T00:59:14Z http://psasir.upm.edu.my/id/eprint/33877/ Screening of tropical medicinal plants for sporicidal activity Lau, Kah Yan Rukayadi, Yaya Bacterial spores have special significance in foods because they are much more resistant to physical and chemical antimicrobial treatment. Nowadays, there is interest in using natural products such as plant extract for food preservation. In this study, 26 of tropical medicinal plants and spices were screened for their sporicidal activity against the spores of Bacillus cereus. The spores of B. cereus was harvested after incubation at 30°C for 1 week and treated with various plant extracts using the method of Standard Operating Procedure for the AOAC (Association of Official Analytical Chemists) Sporicidal Activity. Glutaraldehyde was used as a positive control. Among them, Indonesian bay leaf (Eugenia polyantha Wight) inactivated more than 3 log of spores/ml of B. cereus (99.99%) at the concentration of 1% and completely killed B. cereus spores at concentration of 2.5%. These results suggest that Indonesian bay leaf extract has strong sporicidal activity against spores of B. cereus. Faculty of Food Science and Technology, Universiti Putra Malaysia 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/33877/1/%2861%29.pdf Lau, Kah Yan and Rukayadi, Yaya (2015) Screening of tropical medicinal plants for sporicidal activity. International Food Research Journal, 22 (1). pp. 421-425. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/22%20%2801%29%202015/%2861%29.pdf
spellingShingle Lau, Kah Yan
Rukayadi, Yaya
Screening of tropical medicinal plants for sporicidal activity
title Screening of tropical medicinal plants for sporicidal activity
title_full Screening of tropical medicinal plants for sporicidal activity
title_fullStr Screening of tropical medicinal plants for sporicidal activity
title_full_unstemmed Screening of tropical medicinal plants for sporicidal activity
title_short Screening of tropical medicinal plants for sporicidal activity
title_sort screening of tropical medicinal plants for sporicidal activity
url http://psasir.upm.edu.my/id/eprint/33877/1/%2861%29.pdf
work_keys_str_mv AT laukahyan screeningoftropicalmedicinalplantsforsporicidalactivity
AT rukayadiyaya screeningoftropicalmedicinalplantsforsporicidalactivity