A comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses
3H-labelled complementary DNA (cDNA) was reverse transcribed from the RNA of three biologically distinct isolates of bean yellow mosaic virus (BYMV-G, Q, and S) by the random primer method of Taylor et al. [Biochim. Biophys. Acta 442, 324-330 (1976)]. Excess virus RNA was hybridized with each of the...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1981
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| Online Access: | http://psasir.upm.edu.my/id/eprint/33958/1/26.%2033958%20a%20comparison%20of%20the.pdf |
| _version_ | 1796972412874522624 |
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| author | Abu Samah, Norani Randles, J. W. |
| author_facet | Abu Samah, Norani Randles, J. W. |
| author_sort | Abu Samah, Norani |
| collection | UPM |
| description | 3H-labelled complementary DNA (cDNA) was reverse transcribed from the RNA of three biologically distinct isolates of bean yellow mosaic virus (BYMV-G, Q, and S) by the random primer method of Taylor et al. [Biochim. Biophys. Acta 442, 324-330 (1976)]. Excess virus RNA was hybridized with each of the cDNAs and percentage hybrid formation was assayed using the single-strand-specific nuclease S1, The R0t (1/2) values obtained for the homologous hybridization reactions (1.2 x 10(-2) mol sec liter(-1)) showed that all three BYMV-RNAs were unique species, without detectable zones of reiteration. Reciprocal hybridizations between isolates G, Q, and S have shown that each was significantly different from the other. A comparison of these three isolates with a selection of other potyviruses showed that the relationship between them was closer than that observed between any of them and the other potyviruses tested. |
| first_indexed | 2024-03-06T08:27:38Z |
| format | Article |
| id | upm.eprints-33958 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:27:38Z |
| publishDate | 1981 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-339582015-04-21T08:51:09Z http://psasir.upm.edu.my/id/eprint/33958/ A comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses Abu Samah, Norani Randles, J. W. 3H-labelled complementary DNA (cDNA) was reverse transcribed from the RNA of three biologically distinct isolates of bean yellow mosaic virus (BYMV-G, Q, and S) by the random primer method of Taylor et al. [Biochim. Biophys. Acta 442, 324-330 (1976)]. Excess virus RNA was hybridized with each of the cDNAs and percentage hybrid formation was assayed using the single-strand-specific nuclease S1, The R0t (1/2) values obtained for the homologous hybridization reactions (1.2 x 10(-2) mol sec liter(-1)) showed that all three BYMV-RNAs were unique species, without detectable zones of reiteration. Reciprocal hybridizations between isolates G, Q, and S have shown that each was significantly different from the other. A comparison of these three isolates with a selection of other potyviruses showed that the relationship between them was closer than that observed between any of them and the other potyviruses tested. Elsevier 1981 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/33958/1/26.%2033958%20a%20comparison%20of%20the.pdf Abu Samah, Norani and Randles, J. W. (1981) A comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses. Virology, 110 (2). pp. 436-444. ISSN 0042-6822 10.1016/0042-6822(81)90074-X |
| spellingShingle | Abu Samah, Norani Randles, J. W. A comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses |
| title | A comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses |
| title_full | A comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses |
| title_fullStr | A comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses |
| title_full_unstemmed | A comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses |
| title_short | A comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses |
| title_sort | comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses |
| url | http://psasir.upm.edu.my/id/eprint/33958/1/26.%2033958%20a%20comparison%20of%20the.pdf |
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