In vitro ultrastructural changes of MCF-7 for metastasise bone cancer and induction of apoptosis via mitochondrial cytochrome C released by CaCO3/Dox nanocrystals
Bones are the most frequent site for breast cancer cells to settle and spread (metastasise); bone metastasis is considered to have a substantial impact on the quality of patients with common cancers. However, majority of breast cancers develop insensitivity to conventional chemotherapy which provide...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34153/1/In%20Vitro%20Ultrastructural%20Changes%20of%20MCF-7%20for%20Metastasise.pdf |
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| author | Kamba, Abdullahi Shafiu Ismail, Maznah Tengku Ibrahim, Tengku Azmi Abu Bakar @ Zakaria, Md Zuki Gusau, Lawal Hassan |
| author_facet | Kamba, Abdullahi Shafiu Ismail, Maznah Tengku Ibrahim, Tengku Azmi Abu Bakar @ Zakaria, Md Zuki Gusau, Lawal Hassan |
| author_sort | Kamba, Abdullahi Shafiu |
| collection | UPM |
| description | Bones are the most frequent site for breast cancer cells to settle and spread (metastasise); bone metastasis is considered to have a substantial impact on the quality of patients with common cancers. However, majority of breast cancers develop insensitivity to conventional chemotherapy which provides only palliation and can induce systemic side effects. In this study we evaluated the effect of free Dox and CaCO3/Dox nanocrystal on MCF-7 breast cancer using MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide), neural red, and lactate dehydrogenase colorimetric assays while DNA fragmentation and BrdU genotoxicity were also examined. Apoptogenic protein Bax, cytochrome C, and caspase-3 protein were analysed. Morphological changes of MCF-7 were determined using contrast light microscope and scanning and transmission electron microscope (SEM and TEM). The findings of the analysis revealed higher toxicity of CaCO3/Dox nanocrystal and effective cells killing compared to free Dox, morphological changes such as formation of apoptotic bodies, membrane blebbing, and absent of microvilli as indicated by the SEM analysis while TEM revealed the presence of chromatin condensation, chromosomal DNA fragmentation, cell shrinkage, and nuclear fragmentation. Results of TUNEL assay verified that most of the cells undergoes apoptosis by internucleosomal fragmentation of genomic DNA whereas the extent of apoptotic cells was calculated using the apoptotic index (AI). Therefore, the biobased calcium carbonate nanocrystals such as Dox carriers may serve as an alternative to conventional delivery system. |
| first_indexed | 2024-03-06T08:28:09Z |
| format | Article |
| id | upm.eprints-34153 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:28:09Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-341532016-11-18T08:24:54Z http://psasir.upm.edu.my/id/eprint/34153/ In vitro ultrastructural changes of MCF-7 for metastasise bone cancer and induction of apoptosis via mitochondrial cytochrome C released by CaCO3/Dox nanocrystals Kamba, Abdullahi Shafiu Ismail, Maznah Tengku Ibrahim, Tengku Azmi Abu Bakar @ Zakaria, Md Zuki Gusau, Lawal Hassan Bones are the most frequent site for breast cancer cells to settle and spread (metastasise); bone metastasis is considered to have a substantial impact on the quality of patients with common cancers. However, majority of breast cancers develop insensitivity to conventional chemotherapy which provides only palliation and can induce systemic side effects. In this study we evaluated the effect of free Dox and CaCO3/Dox nanocrystal on MCF-7 breast cancer using MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide), neural red, and lactate dehydrogenase colorimetric assays while DNA fragmentation and BrdU genotoxicity were also examined. Apoptogenic protein Bax, cytochrome C, and caspase-3 protein were analysed. Morphological changes of MCF-7 were determined using contrast light microscope and scanning and transmission electron microscope (SEM and TEM). The findings of the analysis revealed higher toxicity of CaCO3/Dox nanocrystal and effective cells killing compared to free Dox, morphological changes such as formation of apoptotic bodies, membrane blebbing, and absent of microvilli as indicated by the SEM analysis while TEM revealed the presence of chromatin condensation, chromosomal DNA fragmentation, cell shrinkage, and nuclear fragmentation. Results of TUNEL assay verified that most of the cells undergoes apoptosis by internucleosomal fragmentation of genomic DNA whereas the extent of apoptotic cells was calculated using the apoptotic index (AI). Therefore, the biobased calcium carbonate nanocrystals such as Dox carriers may serve as an alternative to conventional delivery system. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34153/1/In%20Vitro%20Ultrastructural%20Changes%20of%20MCF-7%20for%20Metastasise.pdf Kamba, Abdullahi Shafiu and Ismail, Maznah and Tengku Ibrahim, Tengku Azmi and Abu Bakar @ Zakaria, Md Zuki and Gusau, Lawal Hassan (2014) In vitro ultrastructural changes of MCF-7 for metastasise bone cancer and induction of apoptosis via mitochondrial cytochrome C released by CaCO3/Dox nanocrystals. BioMed Research International, 2014. art. no. 391869. pp. 1-14. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2014/391869/abs/ 10.1155/2014/391869 |
| spellingShingle | Kamba, Abdullahi Shafiu Ismail, Maznah Tengku Ibrahim, Tengku Azmi Abu Bakar @ Zakaria, Md Zuki Gusau, Lawal Hassan In vitro ultrastructural changes of MCF-7 for metastasise bone cancer and induction of apoptosis via mitochondrial cytochrome C released by CaCO3/Dox nanocrystals |
| title | In vitro ultrastructural changes of MCF-7 for metastasise bone cancer and induction of apoptosis via mitochondrial cytochrome C released by CaCO3/Dox nanocrystals |
| title_full | In vitro ultrastructural changes of MCF-7 for metastasise bone cancer and induction of apoptosis via mitochondrial cytochrome C released by CaCO3/Dox nanocrystals |
| title_fullStr | In vitro ultrastructural changes of MCF-7 for metastasise bone cancer and induction of apoptosis via mitochondrial cytochrome C released by CaCO3/Dox nanocrystals |
| title_full_unstemmed | In vitro ultrastructural changes of MCF-7 for metastasise bone cancer and induction of apoptosis via mitochondrial cytochrome C released by CaCO3/Dox nanocrystals |
| title_short | In vitro ultrastructural changes of MCF-7 for metastasise bone cancer and induction of apoptosis via mitochondrial cytochrome C released by CaCO3/Dox nanocrystals |
| title_sort | in vitro ultrastructural changes of mcf 7 for metastasise bone cancer and induction of apoptosis via mitochondrial cytochrome c released by caco3 dox nanocrystals |
| url | http://psasir.upm.edu.my/id/eprint/34153/1/In%20Vitro%20Ultrastructural%20Changes%20of%20MCF-7%20for%20Metastasise.pdf |
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