Intracellular production of IFN-alpha 2b in Lactococcus lactis

Human interferon alpha (IFN-α) was expressed in two strains of Lactococcus lactis by aid of two promoters (P32 and Pnis) giving rise to two recombinant strains: MG:IFN and NZ:IFN, respectively. The expression of IFN was confirmed by ELISA and western blotting. Highest production was achieved using g...

Full description

Bibliographic Details
Main Authors: Bayat, Omid, Baradaran, Ali, Ariff, Arbakariya, Mohamad, Rosfarizan, Abdul Rahim, Raha
Format: Article
Published: Springer Netherlands 2014