Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles
A label-free optical detection method based on PNA/DNA hybridization using unmodified gold nanoparticles (AuNPs) for dengue virus detection has been successfully developed. In this study, no immobilization method is involved and the hybridization of PNA/DNA occurs directly in solution. Unmodified Au...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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Hindawi Publishing Corporation
2014
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| _version_ | 1825948430832238592 |
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| author | Abdul Rahman, Samsulida Saadun, Rafidah Azmi, Nur Ellina Ariffin, Nurhayati Abdullah, Jaafar Yusof, Nor Azah Sidek, Hamidah Hajian, Reza |
| author_facet | Abdul Rahman, Samsulida Saadun, Rafidah Azmi, Nur Ellina Ariffin, Nurhayati Abdullah, Jaafar Yusof, Nor Azah Sidek, Hamidah Hajian, Reza |
| author_sort | Abdul Rahman, Samsulida |
| collection | UPM |
| description | A label-free optical detection method based on PNA/DNA hybridization using unmodified gold nanoparticles (AuNPs) for dengue virus detection has been successfully developed. In this study, no immobilization method is involved and the hybridization of PNA/DNA occurs directly in solution. Unmodified AuNPs undergo immediate aggregation in the presence of neutral charge peptide nucleic acid (PNA) due to the coating of PNA on AuNPs surface. However, in the presence of complementary targets DNA, the hybridization of PNA probe with target DNA forms negatively charged complexes due to the negatively charged phosphate backbone of the target DNA. The negatively charged complexes adsorbed onto the AuNPs surface ensure sufficient charge repulsion, need for AuNPs dispersion, and stability in solution. The detection procedure is a naked eye method based on immediate color changes and also through UV-vis adsorption spectra. The selectivity of the proposed method was studied successfully by single base mismatch and noncomplementary target DNA. |
| first_indexed | 2024-03-06T08:28:43Z |
| format | Article |
| id | upm.eprints-34341 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:28:43Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-343412016-02-15T08:29:18Z http://psasir.upm.edu.my/id/eprint/34341/ Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles Abdul Rahman, Samsulida Saadun, Rafidah Azmi, Nur Ellina Ariffin, Nurhayati Abdullah, Jaafar Yusof, Nor Azah Sidek, Hamidah Hajian, Reza A label-free optical detection method based on PNA/DNA hybridization using unmodified gold nanoparticles (AuNPs) for dengue virus detection has been successfully developed. In this study, no immobilization method is involved and the hybridization of PNA/DNA occurs directly in solution. Unmodified AuNPs undergo immediate aggregation in the presence of neutral charge peptide nucleic acid (PNA) due to the coating of PNA on AuNPs surface. However, in the presence of complementary targets DNA, the hybridization of PNA probe with target DNA forms negatively charged complexes due to the negatively charged phosphate backbone of the target DNA. The negatively charged complexes adsorbed onto the AuNPs surface ensure sufficient charge repulsion, need for AuNPs dispersion, and stability in solution. The detection procedure is a naked eye method based on immediate color changes and also through UV-vis adsorption spectra. The selectivity of the proposed method was studied successfully by single base mismatch and noncomplementary target DNA. Hindawi Publishing Corporation 2014 Article PeerReviewed Abdul Rahman, Samsulida and Saadun, Rafidah and Azmi, Nur Ellina and Ariffin, Nurhayati and Abdullah, Jaafar and Yusof, Nor Azah and Sidek, Hamidah and Hajian, Reza (2014) Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles. Journal of Nanomaterials, 2014. art. no. 839286. pp. 1-5. ISSN 1687-4110; ESSN: 1687-4129 http://dx.doi.org/10.1155/2014/839286 10.1155/2014/839286 |
| spellingShingle | Abdul Rahman, Samsulida Saadun, Rafidah Azmi, Nur Ellina Ariffin, Nurhayati Abdullah, Jaafar Yusof, Nor Azah Sidek, Hamidah Hajian, Reza Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles |
| title | Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles |
| title_full | Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles |
| title_fullStr | Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles |
| title_full_unstemmed | Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles |
| title_short | Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles |
| title_sort | label free dengue detection utilizing pna dna hybridization based on the aggregation process of unmodified gold nanoparticles |
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