MALDI-target integrated platform for affinity-captured protein digestion
To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, diges...
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| Format: | Article |
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Elsevier
2014
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| _version_ | 1825948448131645440 |
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| author | Ahmad Tajudin, Asilah Adler, Belinda Ekstrom, Simon Marko-Varga, Gyorgy Malm, Johan Lilja, Hans Laurell, Thomas |
| author_facet | Ahmad Tajudin, Asilah Adler, Belinda Ekstrom, Simon Marko-Varga, Gyorgy Malm, Johan Lilja, Hans Laurell, Thomas |
| author_sort | Ahmad Tajudin, Asilah |
| collection | UPM |
| description | To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40 fmol to 1 pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented. |
| first_indexed | 2024-03-06T08:28:59Z |
| format | Article |
| id | upm.eprints-34429 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:28:59Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-344292015-12-15T03:02:55Z http://psasir.upm.edu.my/id/eprint/34429/ MALDI-target integrated platform for affinity-captured protein digestion Ahmad Tajudin, Asilah Adler, Belinda Ekstrom, Simon Marko-Varga, Gyorgy Malm, Johan Lilja, Hans Laurell, Thomas To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40 fmol to 1 pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented. Elsevier 2014-01-07 Article PeerReviewed Ahmad Tajudin, Asilah and Adler, Belinda and Ekstrom, Simon and Marko-Varga, Gyorgy and Malm, Johan and Lilja, Hans and Laurell, Thomas (2014) MALDI-target integrated platform for affinity-captured protein digestion. Analytica Chimica Acta, 807. pp. 1-8. ISSN 0003-2670; ESSN: 1873-4324 http://www.sciencedirect.com/science/article/pii/S0003267013011689 10.1016/j.aca.2013.08.051 |
| spellingShingle | Ahmad Tajudin, Asilah Adler, Belinda Ekstrom, Simon Marko-Varga, Gyorgy Malm, Johan Lilja, Hans Laurell, Thomas MALDI-target integrated platform for affinity-captured protein digestion |
| title | MALDI-target integrated platform for affinity-captured protein digestion |
| title_full | MALDI-target integrated platform for affinity-captured protein digestion |
| title_fullStr | MALDI-target integrated platform for affinity-captured protein digestion |
| title_full_unstemmed | MALDI-target integrated platform for affinity-captured protein digestion |
| title_short | MALDI-target integrated platform for affinity-captured protein digestion |
| title_sort | maldi target integrated platform for affinity captured protein digestion |
| work_keys_str_mv | AT ahmadtajudinasilah malditargetintegratedplatformforaffinitycapturedproteindigestion AT adlerbelinda malditargetintegratedplatformforaffinitycapturedproteindigestion AT ekstromsimon malditargetintegratedplatformforaffinitycapturedproteindigestion AT markovargagyorgy malditargetintegratedplatformforaffinitycapturedproteindigestion AT malmjohan malditargetintegratedplatformforaffinitycapturedproteindigestion AT liljahans malditargetintegratedplatformforaffinitycapturedproteindigestion AT laurellthomas malditargetintegratedplatformforaffinitycapturedproteindigestion |