Optimization of processing parameters for extraction of amylase enzyme from dragon (Hylocereus polyrhizus) peel using response surface methodology
The main goal of this study was to investigate the effect of extraction conditions on the enzymatic properties of thermoacidic amylase enzyme derived from dragon peel. The studied extraction variables were the buffer-to-sample (B/S) ratio (1: 2 to 1: 6, w/w), temperature (-18°C to 25°), mixing time...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34784/1/Optimization%20of%20Processing%20Parameters%20for%20Extraction%20of.pdf |
| _version_ | 1796972574913069056 |
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| author | Amid, Mehrnoush Abd Manap, Mohd Yazid Ahmad Zohdi, Nor Khanani |
| author_facet | Amid, Mehrnoush Abd Manap, Mohd Yazid Ahmad Zohdi, Nor Khanani |
| author_sort | Amid, Mehrnoush |
| collection | UPM |
| description | The main goal of this study was to investigate the effect of extraction conditions on the enzymatic properties of thermoacidic amylase enzyme derived from dragon peel. The studied extraction variables were the buffer-to-sample (B/S) ratio (1: 2 to 1: 6, w/w), temperature (-18°C to 25°), mixing time (60 to 180 seconds), and the pH of the buffer (2.0 to 8.0). The results indicate that the enzyme extraction conditions exhibited the least significant (P < 0.05) effect on temperature stability. Conversely, the extraction conditions had the most significant (P < 0.05) effect on the specific activity and pH stability. The results also reveal that the main effect of the B/S ratio, followed by its interaction with the pH of the buffer, was significant (P < 0.05) among most of the response variables studied. The optimum extraction condition caused the amylase to achieve high enzyme activity (648.4 U), specific activity (14.2 U/mg), temperature stability (88.4%), pH stability (85.2%), surfactant agent stability (87.2%), and storage stability (90.3%). |
| first_indexed | 2024-03-06T08:30:02Z |
| format | Article |
| id | upm.eprints-34784 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:30:02Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-347842016-12-23T08:00:32Z http://psasir.upm.edu.my/id/eprint/34784/ Optimization of processing parameters for extraction of amylase enzyme from dragon (Hylocereus polyrhizus) peel using response surface methodology Amid, Mehrnoush Abd Manap, Mohd Yazid Ahmad Zohdi, Nor Khanani The main goal of this study was to investigate the effect of extraction conditions on the enzymatic properties of thermoacidic amylase enzyme derived from dragon peel. The studied extraction variables were the buffer-to-sample (B/S) ratio (1: 2 to 1: 6, w/w), temperature (-18°C to 25°), mixing time (60 to 180 seconds), and the pH of the buffer (2.0 to 8.0). The results indicate that the enzyme extraction conditions exhibited the least significant (P < 0.05) effect on temperature stability. Conversely, the extraction conditions had the most significant (P < 0.05) effect on the specific activity and pH stability. The results also reveal that the main effect of the B/S ratio, followed by its interaction with the pH of the buffer, was significant (P < 0.05) among most of the response variables studied. The optimum extraction condition caused the amylase to achieve high enzyme activity (648.4 U), specific activity (14.2 U/mg), temperature stability (88.4%), pH stability (85.2%), surfactant agent stability (87.2%), and storage stability (90.3%). Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34784/1/Optimization%20of%20Processing%20Parameters%20for%20Extraction%20of.pdf Amid, Mehrnoush and Abd Manap, Mohd Yazid and Ahmad Zohdi, Nor Khanani (2014) Optimization of processing parameters for extraction of amylase enzyme from dragon (Hylocereus polyrhizus) peel using response surface methodology. The Scientific World Journal, 2014. art. no. 640949. pp. 1-12. ISSN 2356-6140; ESSN: 1537-744X http://www.hindawi.com/journals/tswj/2014/640949/abs/ 10.1155/2014/640949 |
| spellingShingle | Amid, Mehrnoush Abd Manap, Mohd Yazid Ahmad Zohdi, Nor Khanani Optimization of processing parameters for extraction of amylase enzyme from dragon (Hylocereus polyrhizus) peel using response surface methodology |
| title | Optimization of processing parameters for extraction of amylase enzyme from dragon (Hylocereus polyrhizus) peel using response surface methodology |
| title_full | Optimization of processing parameters for extraction of amylase enzyme from dragon (Hylocereus polyrhizus) peel using response surface methodology |
| title_fullStr | Optimization of processing parameters for extraction of amylase enzyme from dragon (Hylocereus polyrhizus) peel using response surface methodology |
| title_full_unstemmed | Optimization of processing parameters for extraction of amylase enzyme from dragon (Hylocereus polyrhizus) peel using response surface methodology |
| title_short | Optimization of processing parameters for extraction of amylase enzyme from dragon (Hylocereus polyrhizus) peel using response surface methodology |
| title_sort | optimization of processing parameters for extraction of amylase enzyme from dragon hylocereus polyrhizus peel using response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/34784/1/Optimization%20of%20Processing%20Parameters%20for%20Extraction%20of.pdf |
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