Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia
Aeromonas infections are becoming a major risk factor in commercial aquaculture and it has been reported that a wide variety of fish and shellfish species are susceptible to this infection. In this study, 3 isolates of Aeromonas hydrophila were isolated from giant freshwater prawn (Macrobrachium ros...
| Hlavní autoři: | , , , , |
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| Médium: | Článek |
| Jazyk: | English |
| Vydáno: |
Iranian Fisheries Science Research Institute
2015
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| On-line přístup: | http://psasir.upm.edu.my/id/eprint/34838/1/Pathogenicity%20of%20Aeromonas%20hydrophila%20in%20giant%20freshwater%20prawn.pdf |
| _version_ | 1825948528393846784 |
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| author | Sabili, Abdolnabi Md Yasin, Ina Salwany Mohd. Daud, Hassan Shamsudin, Mariana Nor Abdelhadi, Yasser |
| author_facet | Sabili, Abdolnabi Md Yasin, Ina Salwany Mohd. Daud, Hassan Shamsudin, Mariana Nor Abdelhadi, Yasser |
| author_sort | Sabili, Abdolnabi |
| collection | UPM |
| description | Aeromonas infections are becoming a major risk factor in commercial aquaculture and it has been reported that a wide variety of fish and shellfish species are susceptible to this infection. In this study, 3 isolates of Aeromonas hydrophila were isolated from giant freshwater prawn (Macrobrachium rosenbergii) cultured in Kuala Pilah Simbilan Nigri in East Malaysia. Conventional and rapid identification systems (API 20E strips) were used for preliminary identification based on the biochemical characters of the isolated bacteria. On the other hand, polymerase chain reaction (PCR) using the universal primer; 16S rRNA, was done as an accurate and confirmatory identification. The virulence of A. hydrophila was determined using a pathogenicity test via I/M injection. The results revealed that the isolated bacteria were identified as A. hydrophila that revealed a high degree of similarity (98%) to the NCBI or Genbank database. Based on pathogenicity test results, LD50 was determined as 1×106 CFU/50µl, while 1×107 CFU/50µl induced 100% mortality in the experimentally injected prawns. Histopathological changes were found in several organs including gill, hepatopancreas and heart. Those changes were mainly, melanisation, tissue erosion and necrosis, infiltration and hyperplasia of gill lamellae and mild or massive haemocyte reaction in the infected organs. |
| first_indexed | 2024-03-06T08:30:15Z |
| format | Article |
| id | upm.eprints-34838 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:30:15Z |
| publishDate | 2015 |
| publisher | Iranian Fisheries Science Research Institute |
| record_format | dspace |
| spelling | upm.eprints-348382016-01-22T02:59:12Z http://psasir.upm.edu.my/id/eprint/34838/ Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia Sabili, Abdolnabi Md Yasin, Ina Salwany Mohd. Daud, Hassan Shamsudin, Mariana Nor Abdelhadi, Yasser Aeromonas infections are becoming a major risk factor in commercial aquaculture and it has been reported that a wide variety of fish and shellfish species are susceptible to this infection. In this study, 3 isolates of Aeromonas hydrophila were isolated from giant freshwater prawn (Macrobrachium rosenbergii) cultured in Kuala Pilah Simbilan Nigri in East Malaysia. Conventional and rapid identification systems (API 20E strips) were used for preliminary identification based on the biochemical characters of the isolated bacteria. On the other hand, polymerase chain reaction (PCR) using the universal primer; 16S rRNA, was done as an accurate and confirmatory identification. The virulence of A. hydrophila was determined using a pathogenicity test via I/M injection. The results revealed that the isolated bacteria were identified as A. hydrophila that revealed a high degree of similarity (98%) to the NCBI or Genbank database. Based on pathogenicity test results, LD50 was determined as 1×106 CFU/50µl, while 1×107 CFU/50µl induced 100% mortality in the experimentally injected prawns. Histopathological changes were found in several organs including gill, hepatopancreas and heart. Those changes were mainly, melanisation, tissue erosion and necrosis, infiltration and hyperplasia of gill lamellae and mild or massive haemocyte reaction in the infected organs. Iranian Fisheries Science Research Institute 2015 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34838/1/Pathogenicity%20of%20Aeromonas%20hydrophila%20in%20giant%20freshwater%20prawn.pdf Sabili, Abdolnabi and Md Yasin, Ina Salwany and Mohd. Daud, Hassan and Shamsudin, Mariana Nor and Abdelhadi, Yasser (2015) Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia. Iranian Journal of Fisheries Sciences , 14 (1). pp. 232-245. ISSN 1562-2916 http://www.jifro.ir/browse.php?a_id=1857&sid=1&slc_lang=en |
| spellingShingle | Sabili, Abdolnabi Md Yasin, Ina Salwany Mohd. Daud, Hassan Shamsudin, Mariana Nor Abdelhadi, Yasser Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia |
| title | Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia |
| title_full | Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia |
| title_fullStr | Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia |
| title_full_unstemmed | Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia |
| title_short | Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia |
| title_sort | pathogenicity of aeromonas hydrophila in giant freshwater prawn macrobrachium rosenbergii cultured in east malaysia |
| url | http://psasir.upm.edu.my/id/eprint/34838/1/Pathogenicity%20of%20Aeromonas%20hydrophila%20in%20giant%20freshwater%20prawn.pdf |
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